The invention discloses a preparation method of pig
thymosin, which comprises the following steps: 1) mixing water for injection or water for injection of which the pH value is adjusted to 1.5-6.5 by
hydrochloric acid with minced pig thymus, and homogenizing by a
colloid mill to obtain homogenate; 2) carrying out
cell disruption and water insulation and heat preservation on the homogenate, and centrifuging and collecting a centrifugal supernatant; (3) carrying out tangential flow
filtration on the harvested centrifugal supernate by adopting a filter membrane, and collecting permeate; 4) carrying out tangential flow
filtration on the permeate step by step by using filter membranes with different interception pore diameters and molecular weights, and collecting the permeate to obtain a pig
thymosin crude product in a required molecular
weight range; 5) mixing the
thymosin crude products in at least one molecular
weight range in the step 4) to obtain a thymosin primary product; and 6) removing viruses from the pig thymosin primary product, adjusting the pH value and
osmotic pressure, filtering and sterilizing to obtain the pig thymosin. According to the method, step-by-step tangential flow
filtration is adopted, an accurate and effective pig thymosin separation and concentration technology and a
small molecule purification technology are established, and the pig thymosin in the required molecular
weight range can be prepared by selecting filter membranes with different
cut-off pore diameters and molecular weights according to different technological processes.