The invention relates to a separation process for magnetic red mixed ore based on iron material disseminated particle size treatment. The process comprises the operation of feeding the mixed ore withthe grade of 27%-29% and with magnetite as a main raw material into first-section closed circuit ore grinding operation to obtain a primary grading overflow product, and is characterized by further comprising first-section weak magnetic and strong magnetic operation, fine screening operation, first-section magnetic separation and first-section washing magnetic separation operation, second-sectionclosed circuits ore grinding operation, second-section weak magnetic strong magnetic operation, second-section magnetic separation and washing magnetic separation operation, third-section closed circuit ore grinding, third-section weak magnetic strong magnetic operation and reverse flotation operation. The final concentrate with the grade of 66%-66.5% is obtained through the operation. Compared with the prior art, the separation process has the advantages that 1) an efficient separation process which is characterized in that the concentrate can be obtained once dissociation is achieved and segmented ore grinding, segmented concentrate taking and segmented tailings discharging are achieved is provided for the magnetic red mixed ore with the non-uniform iron material disseminated particle sizes, so that overgrinding is reduced, and energy conservation and consumption reduction are achieved; 2) magnetic separation equipment is adopted for upgrading, and three sections of ore grinding is carried out by adopting vertical mills; and 3) the process is simple in structure, free of middling circulation, easy to implement on site and easy to produce and manage.