Glutathion production method

Abstract

The invention relates to a method for producing glutathione, which comprises the following steps: doing large scale culture and separating purify technology to two types of expressing recombining gene's gene project bacillus coli with glutathione enzyme A and enzyme B to obtain enzyme A and enzyme B and doing solid to them by suit materials (1); mixing the two solid enzymes with the ratio EA: EB=1:10-10:1 into the GSH synthesis reactor, which is used in the synthesis of glutathione (2); forming the total producing system into a large fluid cycle type reaction system because GSH synthesis reactor and ATP re-generating reactor are fluid cycle(3); the aminothiopropionic acid availability ratio is above 40%; the glutathione recovery ratio s above 50%; the main synthesis reacting stage can hold the density of glutathione above 0.8 g / L.

Description

technical field [0001] The invention relates to a production method of glutathione. Background technique [0002] Glutathione (GSH) widely exists in animal, plant and microbial cells. It and SOD are the two major reduction systems of the human body. It has important physiological functions such as participating in the transmembrane transport of amino acids and maintaining the reduction state of cells. GSH acts as a sulfhydryl buffer in erythrocytes, maintaining the cysteine ​​residues of hemoglobin and other erythrocyte proteins in a reduced state. GSH is widely used in the food field: GSH content in food industry flour products is usually low, adding GSH to it can greatly enhance its nutritional value; adding it to dairy products can effectively prevent enzymatic and non-enzymatic browning, which is equivalent to vitamin C, play a stabilizing role; adding GSH to fish and poultry food processing can inhibit the decomposition of nucleic acid, strengthen food flavor and great...

AI Technical Summary

Problems solved by technology

[0024] However, in these studies, there is a problem of feedback inhibition in the process of glutathione synthesis, which is difficult to solve at present.