40 results about "Glutathione synthesis" patented technology

Nutritional or therapeutic compositions containing glutamic acid, cystine, glycine and a selenium precursor and methods for their utilization to increase glutathione synthesis and thereby enhance the immune system are described.

The present invention provides uses of multi-nutrient supplements to rescue aberrant biochemical pathways and reduce birth defect caused by maternal diabetes. Choice of supplements is based on the ability of each supplement to correct the following hyperglycemia-associated abnormalities: increased reactive oxygen species generation, abnormal membrane phospholipid metabolism, and decreased glutathione synthesis.

There is provided a method of reducing insulin resistance in a mammalian patient comprising selecting a patient suffering from insulin resistance and administering a compound which increases hepatic glutathione and a compound which increases hepatic nitric oxide.

Nutritional or therapeutic compositions containing glutamic acid, cystine, glycine and a selenium precursor and methods for their utilization to increase glutathione synthesis and thereby enhance the immune system are described.

Topical personal care compositions containing amino acids and nicotinamide for potentiating glutathione synthesis within skin cells. The compositions can be used to improve skin appearance of chronological aging or photoaging, resulting from exposure to UV light / sunlight, or environmental pollutants. The compositions are also useful for attaining even skin color and reducing pigmentation, age spots and discoloration.

A composition for application to the skin can stimulate the in vivo synthesis of collagen and proteoglycans and improve the appearance of the skin, increasing its elasticity and fullness. In general, a composition according to the present invention comprises: (1) an antioxidant compound in a quantity sufficient to enhance collagen synthesis in the skin; (2) an organic penetrant in which the antioxidant compound is soluble in a sufficient quantity that a concentration of the antioxidant compound sufficient to enhance collagen synthesis can be applied topically and penetrate the skin; (3) a mixture of essential amino acids or hydrolyzed whey protein; (4) a supplemental source of sulfur; and (5) a topical pharmaceutically acceptable carrier. The antioxidant compound can be lipoic acid or a lipoic acid analogue or derivative. The organic penetrant is preferably benzyl alcohol. Other ingredients, such as esters of tocopherol and ascorbic acid, can be included.

Specific plant extracts are applied topically to upregulate genes which code for proteins to prevent the build-up of and mitigate the activity of various harmful materials within the skin. These upregulated proteins include glutathione transferases, peroxiredoxins, oxidoreductases, glutaredoxins and glutathione syntheses. These proteins produce additional glutathione, glyoxalase I, maintain cellular redox homeostasis, reduce hydrogen peroxide and alkylhydroperoxides, and conjugate glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Keratinocytes have been induced to significantly upregulate glutathione and glyoxalase I protein expression. Additionally, keratinocytes pre-treated with diallyl trisulfide have increased viability when exposed to ozone, UV radiation or cigarette smoke extract. The active material can be extracted from plants such as garlic, onions, shallots, leeks, chives, scallions, brussel sprouts, broccoli, cabbage, cauliflower, bok choy, kale, mustard, turnip or radish, may include any compound of the allyl sulfide family, and may be of synthetic origin.

The invention discloses a catalytic system coupled with an ATP regeneration system and application of the catalytic system in glutathione production. The application includes: building heterogenous expression / genome integrated Escherichia coli containing glutathione synthesis difunctional enzyme and ATP regeneration enzyme, performing glutathione synthesis reaction condition and catalytic system optimization, and using the mixed culture of the two kinds of Escherichia coli and strain proportion and catalytic reaction process optimization to achieve glutathione production, and raw material, equipment and synthesize reaction costs are saved to the maximum extent.

Glutathione (GSH) is a tripeptide of extreme importance as a catalyst, reductan, and reactant. It can be depleted intra-cellularly either by forming a direct complex with an electrophilic agent (accomplished investigationally by agents such as bromobenzene or diethyl maleate), by way of inhibition of synthesis, or by subjecting cells to oxidant stress. Most cells, except for epithelia cells, do not have a direct transport capacity for intact GSH. Non-epithelial cells must either transport precursor substrates for GSH synthesis or salvage amino acids from circulating GSH for reuse in intracellular resynthesis. Dietary cysteine is a rate limiting substrate for the synthesis of glutathione and also inhibits GSH efflux. Although GSH is synthesized from precursors in virtually all cells, the liver is the main source of plasma GSH. Protection and support of liver function is paramount to elevating GSH levels. The disclosure is also of a unique combination of nutritional supplements including n-acetyl cysteine, vitamin C, 1-glucosamine, n-acetyl d-glucosamine, quercitin, sylimarin, Alpha lipoic acid and high protein, low fat whey that are combined to support various bodily systems involved in glutathione synthesis, reutilization and storage; all intended to elevate glutathione concentration in the mammalian cell.

A method for promoting the synthesis of glutathione by microbial enzymatic method relates to the technical field of synthesizing high-value useful compounds by using microbial cell enzymatic method. Taking recombinant Escherichia coli enzymatic synthesis of glutathione as an example, the present invention uses cultured recombinant Escherichia coli WSH-KE1 cells as the enzyme source, and directly adds low-concentration organic solvents or surfactants to the reaction system, To reduce the permeability barrier of the outer cell membrane, in the presence of adenosine triphosphate (ATP), catalyze L-Glu, L-Cys and Gly to synthesize glutathione, and the glutathione synthesis amount can reach 4.8g / L after 2 hours of reaction. The invention simplifies the procedure of microbial cell permeability treatment, avoids the possibility of loss of intracellular substances during centrifugation caused by the damage of the permeabilizer to the cell membrane or the lysis of the cells, and ultimately increases the glutathione production.

The invention belongs to the technical field of nutritional products, and particularly provides functional food for improving concentration of glutathione in a human body and a preparation method of the functional food. The functional food comprises a component A and a gain agent, wherein the component A consists of cystine, cystine-contained polypeptide and / or protein, glycine-contained polypeptide and / or protein as well as glutamic-acid-contained polypeptide and / or protein. With the adoption of the functional food for improving the concentration of glutathione in the human body and the preparation method of the functional food, glutathione synthesis in the human body can be promoted, and the concentration level of glutathione in the human body is improved, so that immune capacity of the human body is improved, and further, antioxidation and detoxication are achieved. The component A and the gain agent are matched for use, so as to more effectively improve the concentration of glutathione and the antioxidant effect. Protein and polypeptide are directly obtained from natural food, the taste is better, accordingly, the functional food is more suitable for being eaten by a human, and moreover, other essential amino acids for the human body are contained, so that the functional food is more beneficial to health of the human body; and glutathione is an important antioxidant in the human body, cellular oxidation is the cause of senility and various diseases, therefore, the functional food can be applied to prevention and adjuvant therapy of various diseases.

The invention relates to a recombinant plasmid for promoting glutathione synthesis by dynamically regulating ATP, an engineering bacterium and an application thereof, and belongs to the technical field of fermentation. In the invention, a gene recombination technology is utilized; firstly, a riboswitch ydaO module, a ppk gene, a gshf gene segment and a plasmid vector are recombined to construct arecombinant plasmid; and then, the recombinant plasmid is transferred into an escherichia coli competent state, and an ATP regulation and control method based on the riboswitch ydaO module and polyphosphokinase (ppk) is constructed in escherichia coli so that an ATP concentration can be controlled at a certain level, and synthesis of intracellular ATP dependent products is facilitated.

The present invention provides uses of multi-nutrient supplements to rescue aberrant biochemical pathways and reduce birth defect caused by maternal diabetes. Choice of supplements is based on the ability of each supplement to correct the following hyperglycemia-associated abnormalities: increased reactive oxygen species generation, abnormal membrane phospholipid metabolism, and decreased glutathione synthesis.

There is provided a method of reducing insulin resistance in a mammalian patient comprising selecting a patient suffering from insulin resistance and administering a compound which increases hepatic glutathione and a compound which increases hepatic nitric oxide.

Topical personal care compositions containing amino acids and 4-substituted resorcinols for potentiating glutathione synthesis within skin cells. The compositions can be used to improve skin appearance of chronological aging or photoaging, resulting from exposure to UV light / sunlight, or environmental pollutants. The compositions are also useful for attaining even skin color and reducing pigmentation, age spots and discoloration.

The invention discloses a construction method of recombinant plasmids for glutathione biosynthesis, and relates to the technical field of bioengineering, the recombinant plasmids constructed by the method are saccharomyces cerevisiae double-gene co-expression plasmids, an OAS anabolism pathway of cysteine is introduced into saccharomyces cerevisiae cells through overexpression of serine O-acetyltransferase (SATase), synthesis of endogenous cysteine of saccharomyces cerevisiae can be promoted, the glutathione synthesis capacity of the saccharomyces cerevisiae in combination with overexpression of gamma-glutamylcysteine synthetase (GSH I) can be improved, and the growth speed of the saccharomyces cerevisiae in the logarithmic phase can be accelerated while the yield of the saccharomyces cerevisiae glutathione can be effectively improved.

The invention relates to a method for culturing selenium-enrichment and glutathione-enrichment cucumbers. The method includes the steps that 1, when cucumber seedlings are cultured, organic compound fertilizers special for the selenium-enrichment and glutathione-enrichment cucumbers are adopted, the seedling culturing density is 150 to 200 per square meter, and the applied fertilizer quantity ranges from 8 kg per mu to 10 kg per mu; 2, the seedlings which are obtained in the first step of seedling culturing and each have three to four main leaves are planted, and before the seedlings are planted, organic compound fertilizers special for the selenium-enrichment and glutathione-enrichment cucumbers are applied to culturing land, and after the fertilizers are applied, the land is turned over and leveled, wherein the applying quantity ranges from 150 kg per mu to 200 kg per mu, when planting is carried out, the planting distance ranges from 50 cm to 60 cm, and double-line culturing is carried out; 3, plants are adjusted; 4, additional fertilizing is carried out, wherein when fruits begin to be borne, organic compound fertilizers special for the selenium-enrichment and glutathione-enrichment cucumbers are additionally applied, and the additionally-applying quantity ranges from 100 kg per mu to 150 kg per mu; 5, harvesting is carried out. According to the method, sufficient glutathione synthesis precursors and sufficient selenium sources are provided for the cucumbers from the seedling culturing stage, the cucumbers can absorb nutrition from the land in the whole growing period and convert the nutrition into glutathione and organic selenium through photosynthesis, and the content of the glutathione and the content of the organic selenium in the cucumbers are high.

The invention discloses a series of mutants of bifunctional glutathione synthetase and an application of the mutants in glutathione synthesis. The series of mutants are obtained by single-point mutation or combined mutation on amino acids at 29th, 440th, 442nd, 483rd, 488th, 573rd and 576th sites of bacillus cereus bifunctional glutathione synthetase, wherein activity of the M4 mutant with the highest activity is 26.6 times higher than that of wild glutathione synthetase, glutathione synthesized by the M4 mutant under enzymatic synthesis has the highest concentration reaching 24.8 g / L, whichis 5.76 times higher than that of the wild glutathione synthetase, the production cost is greatly reduced, and the mutants have great application value.

Disclosed are methods of treating hyperproliferative disorders such as cancer, methods of arresting the cell cycle in cancer cells, methods of inhibiting glutathione synthesis in cancer cells, and associated compounds for use and uses in medicaments. In certain embodiments, the methods, uses and compounds are provided with reference to compounds of the structural formula (I), in which X1, X2, Z1, Z2, the ring system denoted by “a”, R1, L1, L2, Q, L3, R3, L4, R4, L5, and R5 are as described herein. In certain embodiments, compounds disclosed herein are especially active against cancers having a mutant KRAS gene.

The invention relates to the field of polypeptide synthesis, in particular to an immobilized fusion enzyme and a method for preparing glutathione with it. In the present invention, the glutathione synthetase gene, the adenosine triphosphate regenerating enzyme gene and the chitin protein domain gene are respectively connected and expressed to prepare a fusion enzyme, and the chitin carrier can be used to specifically and firmly bind the chitin protein domain , realize one-step purification of immobilized glutathione synthesis fusion enzyme and adenosine triphosphate regeneration fusion enzyme, the process is simple, and the space density of immobilized enzyme is high. The immobilized fusion enzyme prepared by the present invention has high enzyme activity and can realize continuous production of glutathione. The present invention adopts the batch and continuous process route of the immobilized fusion enzyme to generate glutathione up to 31g / L. The target The product concentration is high, and there is no enzyme residue in the product solution, which greatly simplifies the later purification process.

Disclosed are compounds useful, for example, in methods of treating hyperproliferative disorders such as cancer, methods of arresting the cell cycle in cancer cells, methods of inhibiting glutathione synthesis in cancer cells, and associated compounds for use and uses in medicaments. In certain embodiments, the methods, uses and compounds are provided with reference to compounds of the structural formulae (Ia), (Ib), (Ic), (Id) and (Ie), in which R1, L1, L2, Q, L3, R3, L4, R4, L5, and R5 are as described herein. In certain embodiments, compounds disclosed herein are especially active against cancers having a mutant KRAS gene.

Topical personal care compositions containing amino acids and 4-substituted resorcinols for potentiating glutathione synthesis within skin cells. The compositions can be used to improve skin appearance of chronological aging or photoaging, resulting from exposure to UV light / sunlight, or environmental pollutants. The compositions are also useful for attaining even skin color and reducing pigmentation, age spots and discoloration.

The invention relates to the field of polypeptide synthesis, and in particular relates to immobilized fusion enzyme and a method for preparing glutathione by using the immobilized fusion enzyme. According to the invention, a glutathione synthetase gene and a triphosadenine regenerative enzyme gene are respectively connected with a chitin protein domain gene; fusion enzyme is prepared by expression; by utilization of the fact that a chitin carrier can be bound to a chitin protein domain specifically and firmly, glutathione synthesis fusion enzyme and triphosadenine regenerative fusion enzyme are purified and immobilized through one step; the process is simple; and the space density of the immobilized enzyme is high. The immobilized fusion enzyme prepared by the invention is high in enzyme activity; continuous generation of glutathione can be realized; the batch and continuous process route of the immobilized fusion enzyme is adopted in the invention; glutathione up to 31 g / L can be generated; the concentration of a target product is high; no enzyme residue exists in product solution; and the later purification process is greatly simplified.

The invention relates to a method for culturing selenium-enrichment and glutathione-enrichment cucumbers. The method includes the steps that 1, when cucumber seedlings are cultured, organic compound fertilizers special for the selenium-enrichment and glutathione-enrichment cucumbers are adopted, the seedling culturing density is 150 to 200 per square meter, and the applied fertilizer quantity ranges from 8 kg per mu to 10 kg per mu; 2, the seedlings which are obtained in the first step of seedling culturing and each have three to four main leaves are planted, and before the seedlings are planted, organic compound fertilizers special for the selenium-enrichment and glutathione-enrichment cucumbers are applied to culturing land, and after the fertilizers are applied, the land is turned over and leveled, wherein the applying quantity ranges from 150 kg per mu to 200 kg per mu, when planting is carried out, the planting distance ranges from 50 cm to 60 cm, and double-line culturing is carried out; 3, plants are adjusted; 4, additional fertilizing is carried out, wherein when fruits begin to be borne, organic compound fertilizers special for the selenium-enrichment and glutathione-enrichment cucumbers are additionally applied, and the additionally-applying quantity ranges from 100 kg per mu to 150 kg per mu; 5, harvesting is carried out. According to the method, sufficient glutathione synthesis precursors and sufficient selenium sources are provided for the cucumbers from the seedling culturing stage, the cucumbers can absorb nutrition from the land in the whole growing period and convert the nutrition into glutathione and organic selenium through photosynthesis, and the content of the glutathione and the content of the organic selenium in the cucumbers are high.

The invention relates to a recombinant plasmid used for synthesizing glutathione and a preparation method thereof. The recombinant plasmid comprises a GshF fragment and a carrier fragment, and the carrier fragment is pET-22b. The recombinant plasmid can not only express the glutathione efficiently, but also reduce the degradation of the glutathione, the efficiency of glutathione synthesis is thusimproved, and the production cost and the energy consumption are reduced.