Method for promoting microbial enzyme process to synthesizing glutathione

Abstract

A method for promoting the synthesis of glutathione by microbial enzymatic method relates to the technical field of synthesizing high-value useful compounds by using microbial cell enzymatic method. Taking recombinant Escherichia coli enzymatic synthesis of glutathione as an example, the present invention uses cultured recombinant Escherichia coli WSH-KE1 cells as the enzyme source, and directly adds low-concentration organic solvents or surfactants to the reaction system, To reduce the permeability barrier of the outer cell membrane, in the presence of adenosine triphosphate (ATP), catalyze L-Glu, L-Cys and Gly to synthesize glutathione, and the glutathione synthesis amount can reach 4.8g / L after 2 hours of reaction. The invention simplifies the procedure of microbial cell permeability treatment, avoids the possibility of loss of intracellular substances during centrifugation caused by the damage of the permeabilizer to the cell membrane or the lysis of the cells, and ultimately increases the glutathione production.

Description

technical field [0001] A method for promoting the synthesis of glutathione by microbial enzymatic method, by directly adding a penetrating agent to the reaction system, reducing the permeability barrier of the outer membrane of cells, promoting the synthesis of glutathione by microbial cell enzymatic method, and increasing the concentration of glutathione. The peptide yield relates to the technical field of synthesizing high-value useful compounds by using microbial cell enzymes. Background technique [0002] The use of microbial whole-cell biocatalysis to synthesize high-value useful compounds has attracted more and more attention because of its potential for large-scale industrial applications. Such as the enzymatic synthesis of glutathione by genetically engineered bacteria. By cloning the genes gsh I and gsh II encoding γ-glutamylcysteine ​​synthetase and glutathione synthase, and constructing a recombinant Escherichia coli with high glutathione synthesis activity, and ...

AI Technical Summary

Problems solved by technology

These methods are mainly permeabilization pretreatment. The treatment procedure is mainly permeabilization treatment-centrifugation-washing and other operations. The treatment process is relatively cumbersome. Phenomena such as cracking, resulting in the loss of microbial intracellular substances during centrifugation, reducing the efficiency of microbial cell biocatalysis