13 results about "Riboswitch" patented technology

The present disclosure relates to oligonucleotide aptamers that bind to certain small molecules including thiamine analogs and derivatives and methods of generating aptamers that bind to the small molecules. Also contemplated are riboswitches and polynucleotide cassettes comprising the aptamers disclosed herein. Further provided are methods of using said aptamers, riboswitches, and / or polynucleotide for the regulation of target genes, including therapeutic genes. Also provided herein are small molecules that are modulators of target gene expression where the target gene contains a riboswitch comprising an aptamer described herein.

Disclosed are a method for screening a riboswitch capable of specifically recognizing doxycycline as well as rational engineering and application of the riboswitch, and belongs to the field of gene expression regulation. The present disclosure provides a doxycycline specific riboswitch, and establishes a high-throughput screening method based on flow cytometry, and obtains the doxycycline riboswitch with further improved activation fold by way of computer-aided prediction of binding sites of the riboswitch and calculation of mutation sites. According to a dose-response curve, the riboswitch shows linear correlation at 40-100 μg / L, and meanwhile, a whole-cell sensor constructed is low in price, high in stability and simple to operate, and can be used for on-site rapid detection.

The invention discloses a riboflavin biosensor construction method based on an RNA element, and belongs to the field of synthetic biology. On the basis of an FMN riboswitch and a foothold switch, an expression platform of a wild type FMN riboswitch is artificially modified, a sensitive riboswitch biosensor is constructed, and efficient detection of the riboflavin concentration is achieved; the fluorescence value of a riboswitch of the detection strain constructed by the method under the riboflavin concentration of 1 mmol / L is 4.3 times of the fluorescence value under the riboflavin concentration of 0 mmol / L.

The present disclosure relates to riboswitches and polynucleotide cassettes that modulate the expression of chimeric antigen receptors (CARs) in response to small molecule ligands, wherein the polynucleotide cassettes comprise the riboswitches and aptamers disclosed herein. Also provided are methods for generating a population of T cells wherein the T cells comprise a CAR transgene comprising an expression construct that inductively expresses a CAR in response to a small molecule inducer that binds to a riboswitch aptamer, which is part of the expression construct. Also provided are methods for treating cancer by administering to a patient in need thereof: (i) a population of T cells comprising an induced CAR comprising the riboswitch described herein; and (ii) a small molecule inducer (aptamer ligand) disclosed herein.

The invention discloses a bacillus amyloliquefaciens riboswitch regulation and control system and application thereof. The regulation and control system is composed of a P43 promoter, a theophylline aptamer TC, RBS, RepA-tag and a required regulation and control gene. A theophylline ribose switch regulation element is integrated on the upstream of a bacillus amyloliquefaciens regulation gene, and the expression quantity of the gene is precisely regulated through theophylline induced expression. Furthermore, a theophylline ribose switch regulation element is integrated to the upstream of the genes hemX and mtnN to obtain the dual-regulation engineering bacterium HZ-B. Under induction of 2mM theophylline, the heme yield and the 5-adenosylmethionine (SAM) yield of the dual-regulation engineering bacterium HZ-B are remarkably increased and are respectively increased by 33.1 times and 0.5 times compared with those of an original strain HZ-12.

This disclosure provides a small molecule of formula (I) that binds to an aptamer. Also intended are riboswitches and polynucleotide cassettes for regulating the expression of a target gene in response to the small molecule, the polynucleotide cassette comprising the aptamer disclosed herein. The small molecule disclosed herein, bound to the aptamer disclosed herein, is a modulator of target gene expression, and the target gene comprises a riboswitch containing the aptamer described herein. JPEG2026524820000284.jpg40164

The present disclosure provides small molecules of formula (I) that bind to aptamers. Also contemplated are riboswitches and polynucleotide cassettes that modulate the expression of a target gene in response to the small molecules, wherein the polynucleotide cassettes comprise the aptamers disclosed herein. The small molecules disclosed herein bound to the aptamers disclosed herein are modulators of expression of a target gene, wherein the target gene contains a riboswitch comprising the aptamers described herein.

The invention provides polynucleotide constructs for the regulation of gene expression by aptamer-based modulation of U1 snRNP-mediated suppression of polyadenylation and methods of using the constructs to regulate gene expression in response to the presence or absence of a ligand that binds the aptamer. The polynucleotide construct contains a U1 binding site in the context of a riboswitch comprising an effector region and an aptamer such that when the aptamer binds a ligand, target gene expression occurs.

The invention provides eukaryotic cell-based screening methods to identify an aptamer that specifically binds a ligand, or a ligand that specifically binds an aptamer, using a polynucleotide cassette for the regulation of the expression of a reporter gene where the polynucleotide cassette contains a riboswitch in the context of a 5′ intron-alternative exon-3′ intron. The riboswitch comprises an effector region and an aptamer such that when the aptamer binds a ligand, reporter gene expression occurs.

The application discloses a riboswitch element responding to vitamin B12 and construction and application of a vitamin B12 biosensor based on the riboswitch element, and belongs to the fields of genetic engineering technology, molecular detection and biosensing. The biosensor comprises a riboswitch element responding to vitamin B12 and a selectable marker gene. An effective relationship between vitamin B12 concentration in a bacterium and expression of the marker gene is established by using the sensor. The biosensor can be used for selecting a high-yield vitamin B12 strain in a relatively fast and easy way, and has an important application prospect in detection and screening of vitamin B12-producing microorganisms.

The invention discloses a microbial whole-cell sensor based on a riboswitch as well as a construction method and application thereof, and belongs to the technical field of biosensors. The invention provides a microbial whole-cell sensor based on a riboswitch, escherichia coli is modified by synthetic biology, so that the escherichia coli responds to mercury ions and expresses red fluorescent protein through the riboswitch, protein signals with different intensities are further generated, and the effect of detecting the mercury ions is achieved; according to the microbial whole-cell sensor based on the riboswitch, mercury ion signals can be quickly responded through the designed artificial riboswitch; through combination with transcription factor MerR, chaperonin ProQ and BL21 Star (DE3) strains, the probability of false positive results can be effectively reduced, the anti-interference capability is improved, and the detection specificity, sensitivity and accuracy of the whole-cell sensor are greatly improved.

The invention discloses a protein loop for targeted killing of CMS2 colon cancer cells as well as a related biological material and application thereof. The invention relates to the technical field of biology, and provides a protein complex for targeted killing of CMS2 type colon cancer cells, which comprises three chimeric proteins of an activation element, a killing element and a regulating element, and is characterized in that: (1) the activation element activates the killing element only in MYC and WNT signal pathway activated cells through a cutting effect; (2) while the regulating element and the activating element are mutually inhibited to form a positive feedback cycle, the cutting effect of the activating element on the killing element is feedforward inhibited, so that the selectivity of a gene loop is increased. The protein loop constructed by the invention can be introduced in a single transcript form, and has excellent integration. Compared with the prior art, the protein loop constructed by the invention can specifically kill CMS2 type colon cancer cells in a targeting manner compared with a gene loop depending on a riboswitch, and has important application value in treatment of CMS2 type colon cancer.

The invention relates to the technical field of fluorine content detection, and discloses a fluorine content detection method for fluoride riboswitch regulation and transcription and application. According to the present invention, with the biological element of the fluoride ribose switch, the fluorine ions in the sample to be detected can be captured and combined with high sensitivity and high specificity, and the fluorine ion concentration can be converted into the fluorescence signal based on the CRISPR technology so as to amplify the fluorine ion concentration signal based on the fluorescence signal. Due to the fact that the combination of the fluoride riboswitch and the transcription reaction are high in efficiency and stability, the detection method can achieve rapid and accurate detection of the fluorine content of the to-be-detected sample. The fluorine content detection method provided by the invention is a method integrating the advantages of low cost, high sensitivity, high specificity, simplicity and convenience in operation, reliable result and the like, and has application prospects in industrial detection and temporary detection scenes.