7 results about "Electron transport chain" patented technology

The invention relates to the technical field of biological denitrification, in particular to a nitrogen-doped carbon dot-based microbial electronic control denitrification system and application thereof. The system comprises electron donor microorganisms, electron acceptor microorganisms and nitrogen-doped carbon dots, the nitrogen-doped carbon dots serve as electron mediators outside cells, an efficient extracellular electron transfer network is constructed, and interspecific electron transfer resistance is reduced; meanwhile, the nitrogen-doped carbon dots can regulate and control intracellular electron transport chains and the energy metabolism process, the improvement of the reducing power and the energy level is promoted, and the metabolism mode optimization is realized. By cooperatively regulating and controlling generation, transmission and utilization of electrons under an extreme electron donor limited oligotrophic condition, the system disclosed by the invention can realize efficient denitrification, avoid nitrite accumulation, remarkably inhibit generation of nitrous oxide (N2O), reduce dependence on an external organic carbon source and an electron source, and improve the denitrification efficiency. And a new technical approach is provided for improving the deep denitrification efficiency under the low-carbon condition.

The application discloses an engineering bacterium, a biosensor and a detection method thereof. The biosensor takes the modified Acinetobacter baylyi ADP1 as a carrier, utilizes the natural nucleic acid uptake capacity thereof, and can autonomously absorb exogenous nucleic acid without external induction; after the nucleic acid is internalized, the hairpin structure restriction of the blocked gRNA is removed, the CRISPRa system is activated, the dCas9-SoxS protein is targeted and combined with the target promoter, the CymA-MtrCAB gene expression is started, and the specific recognition of the nucleic acid sequence is realized. The system integrates the CymA-MtrCAB transmembrane electron conduction module, converts the biological recognition signal into an electrochemical signal output in real time through the membrane protein-mediated electron transfer chain, and establishes a detection path of 'nucleic acid recognition-gene activation-electric response'. The biosensor has the advantages of modular structure, high response specificity, high detection sensitivity, wide environmental adaptability and the like, is suitable for tumor marker detection, wearable health monitoring and other biological molecule recognition scenes, and provides a novel programmable biological perception solution with high universality.

The invention provides a synthetic microbial community and application thereof in degradation of trehalose and biofuel cells, and belongs to the technical field of synthetic microbiology and biological energy. A synthetic microbial community is constructed by means of synthetic biology, the synthetic microbial community comprises genetic engineering escherichia coli and shewanella, and the synthetic microbial community can realize a complete metabolic pathway from trehalose to acetic acid and an efficient electron transport chain; the method can be used for treating trehalose-rich wastewater or wastes and the like; the synthetic microbial community can be used for a microbial fuel cell, can stably generate current density and power density which are higher than those of a single strain or a natural mixed flora in the microbial fuel cell, and has good practicability.

The application relates to the technical field of wastewater treatment, in particular to an integrated catalytic biofilm composite and a wastewater denitrification treatment method, which are prepared by the following method: a photocatalytic carrier is immersed in a heterotrophic nitrification and aerobic denitrification bacteria liquid, adsorption is carried out at 25 DEG C to 30 DEG C for 6 hours to 12 hours, and a bacteria-carrier composite is obtained; then the bacteria-carrier composite is immersed in a crosslinking liquid containing sodium alginate and calcium chloride to carry out crosslinking, and a gel-embedded integrated catalytic biofilm composite is formed; the volume ratio of the bacteria-carrier composite to the crosslinking liquid is 1:5-10; under the driving of visible light, photo-generated electrons are efficiently transferred to the bacteria electron transfer chain, the denitrification enzyme activity is significantly enhanced, and the material surface defect sites selectively degrade antibiotics. The application realizes efficient simultaneous denitrification and antibiotic removal under low-carbon source conditions, has strong system stability and low energy consumption, and is suitable for aquaculture, industrial and urban wastewater treatment.

The invention discloses construction of Escherichia coli with enhanced extracellular electron transfer capability and application of the Escherichia coli in MFC (Microbial Fuel Cell), belongs to the field of gene engineering, and aims to perform targeted knockout on dld genes by utilizing a pEcCas / pEcgRNA mediated gene editing system, block a lactic acid synthesis pathway and promote intracellular accumulation of reducing power NAD (P) H, so as to provide more sufficient driving force for an electron transfer chain. Then, the electricity generation performance of the gene editing strain is evaluated by constructing an MFC platform, the effectiveness of the metabolic engineering strategy is verified, and a new thought and technical basis are provided for the rational design of an MFC microbial electrode.

The invention provides a medicine for targeting early-stage precursor T cell acute lymphocytic leukemia (ETP-ALL) and application of the medicine. Dichloroacetic acid (DCA) is used for regulating mitochondrial oxidative phosphorylation, so that electron transport chain flux can be enhanced, ROS (reactive oxygen species) generation can be promoted, endoplasmic reticulum stress related pathways can be activated, the proliferation states of an ETP-ALL leukemia cell line and a primary patient sample can be influenced, and cell apoptosis can be induced. In cell line-derived and patient-derived xenograft models, DCA treatment can reduce leukemia load in bone marrow, spleen and peripheral blood and affect tumor formation characteristics. DCA shows good tolerance in normal hematopoietic system and other leukemia models. The method provides a treatment strategy based on metabolism targeted regulation, and provides a potential technical route and application reference for ETP-ALL research and drug development.

The invention belongs to the technical field of biological energy and organic solid waste resourceful treatment, and provides a strengthening method for anaerobic methanogenesis. According to the method, magnetotactic bacteria Solidesulfovibrio magneticus RS-1 are introduced into an anaerobic methanogenesis system containing methanogenesis archaea, and a magnetic conductive material is added, so that the magnetotactic bacteria are directionally enriched on the surface of a magnetic material under the magnetic action of the magnetic conductive material and a local magnetic environment formed by the magnetic conductive material; and forming a microbial aggregate with a magnetic material as a core with methanogenic archaea, so that an efficient electron transfer link is constructed in a micro-domain scale, and the methane generation efficiency is improved. The embodiment shows that compared with a control system without magnetotactic bacteria and magnetic materials, the method has the advantages that the methane accumulation yield can be remarkably increased by 30% or above, enrichment of methanogens in magnetic aggregates is promoted, the mass transfer resistance of intermediate metabolites is reduced, and the stability of the system is enhanced.