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Surface cleaning detection reagent for sanitation monitoring

A technology for detection reagents and surfactants, applied in the field of health and epidemic prevention, can solve the problems of poor thermal stability, uneconomical, and large dosage of phosphate-based compounds

Active Publication Date: 2008-02-20
INST OF ELECTRONICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, there has been a domestic patent CN1338003 involving the application of ATP regeneration enzymes to the detection of sample pollutants, but the phosphate-based compounds used have poor thermal stability and a large amount, which may have the problem of low ATP regeneration efficiency and uneconomical

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A set of detection reagents is preferred, and its composition and concentration are as follows.

[0024] The ATP releaser consists of:

[0025] Dodecyltrimethylammonium Bromide 0.2mg / ml

[0026] Fatty alcohol polyoxyethylene (15) ether 1.2mg / ml

[0027] Tris-HCl (20mM) PH7.4

[0028] The composition of the fluorescence inducer without ATP regeneration enzyme is:

[0029] Fluorescein 0.3mg / mL

[0030] Luciferase 0.05mg / mL

[0031] Magnesium chloride 10mM

[0032] Bovine Serum Albumin 0.11mM

[0033] EDTA 2mM

[0034] Tetrasodium pyrophosphate 0.02mM

[0035]Dithiothreitol 1mM

[0036] Cyclodextrin 6mM

[0037] Tris-HCl (50mM) PH7.8

[0038] The above ATP releasing agent and the fluorescence inducer without ATP regenerating enzyme are prepared according to the indicated concentration to prepare a set of detection reagents to quickly detect the surface cleanliness of weighing tools in the laboratory. When testing, first smear the entire surface of the object to...

Embodiment 2

[0040] A set of detection reagents is preferred, and its composition and concentration are as follows.

[0041] The ATP releaser consists of:

[0042] Sodium dodecyl sulfate 0.1mg / ml

[0043] Triton X-100 1mg / ml

[0044] Tris-HCl (20mM) PH7.8

[0045] The composition of the fluorescent inducer containing ATP regenerating enzyme is:

[0046] Fluorescein 0.25mg / mL

[0047] Luciferase 0.04mg / mL

[0048] Magnesium chloride 10mM

[0049] Bovine Serum Albumin 0.11mM

[0050] EDTA 2mM

[0051] Coenzyme A 0.05mM

[0052] Dithiothreitol 1mM

[0053] Polyphosphate compound 0.05mM

[0054] Polyphosphate synthase 0.1U / mL

[0055] Adenylate kinase 0.5U / mL

[0056] Tris-HCl (50mM) PH7.8

[0057] The above ATP releasing agent and the fluorescent inducer containing ATP regenerating enzyme were prepared according to the indicated concentrations to obtain a set of detection reagents to quickly detect the surface cleanliness of the laboratory working environment; at the same time, a...

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Abstract

The utility model discloses a surface cleanliness detection reagent used in health supervision, which is based on the combination of ATP biological fluorescence method and enzymatic cycling amplification technology to realize rapid detection and field assessment of health surface cleanliness. The utility model comprises ATP releaser and a fluorescent inducer with ATP regeneration enzyme; wherein, the ATP releaser can release microorganisms, somatic cells and ATP in product residues; the fluorescent inducer can react with the ATP to produce quantitative fluorescence, and then combined with the ATP regeneration enzyme to amplify the produced fluorescent signals further. The utility model, which has the advantages of convenient use of reagent, simple operation, fast response, high sensitivity and low detection cost, is widely used in health monitoring and health field supervision in food industry, cosmetic industry, medical industry and other industries.

Description

technical field [0001] The invention belongs to the technical field of sanitation and epidemic prevention, relates to sanitation detection and monitoring, in particular to a reagent for rapid detection of sanitation surface cleanliness based on the combination of ATP biofluorescence method and enzyme cycle amplification technology. Background technique [0002] In the normal operation process of food, cosmetics, electronics, medical and other industries, the control and detection of environmental sanitation such as operators' hands, utensils and work surfaces are very important. At present, most enterprises and health supervision agencies mainly evaluate the environmental health status by the number of microorganisms on the surface of the object, and rely on the traditional or improved agar plate culture method for detection, which is slow and inefficient. From collecting samples to obtaining results It takes at least 24 to 48 hours, which cannot meet the requirements of ent...

Claims

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Application Information

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IPC IPC(8): G01N21/76C12Q1/25
Inventor 罗金平蔡新霞岳伟伟何保山周爱玉刘春秀
Owner INST OF ELECTRONICS CHINESE ACAD OF SCI
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