42 results about "Field population" patented technology

Methods and apparatus provide for the production of metadata-based user interfaces (UIs) such as graphical user interfaces (GUIs). In one example, keypad descriptor metadata is obtained. The keypad descriptor metadata is data identifying a plurality of available keypad GUIs for a particular data field to control the change from a first keypad GUI to a different keypad GUI. The first keypad GUI is provided for the data field based on the obtained keypad descriptor metadata. A second and different keypad GUI is also provided for the same data field based on the keypad descriptor metadata during the same field population session. In another example, a user interface is provided for a device. The user interface is changed based on a current machine state of an input/output function of the device and based on user interface descriptor metadata associated with an element of the user interface.

The invention relates to a method for reproducing paphiopedilum armeniacum. The method comprises the steps of carrying out field planting on adult plants of the paphiopedilum armeniacum, carrying out artificial pollination when the paphiopedilum armeniacum flowers, marking the time of pollination and seeding by using capsule seeds of the paphiopedilum armeniacum of 90-110 days after the pollination. In recent years, field populations of the paphiopedilum armeniacum are excessively collected, seriously damaged and endangered and problems of protection and commercial development are needed to solve through artificial breeding method. According to the invention, aiming to solve the problem that the paphiopedilum armeniacum seed is more difficult to sprout after maturing, methods of seed morphological characteristic observing and aseptic germination are utilized to confirm that the seed germination rate is highest when the seed blastocyte morphological characteristic is shown as ellipsoidal embryogenesis and the suspensor presents a character of 2-3 cellular components in linear arrangement after 90 days after the pollination of the paphiopedilum armeniacum, therefore, the method disclosed by the invention has the advantages that optimal germination time of the seeds of the paphiopedilum armeniacum can be confirmed and technological basis is established for the building of a high-efficiency artificial reproduction technical system of the paphiopedilum armeniacum.

The invention provides a method for detecting the drug resistance of adelphocoris suturalis. A diagnosis glass tube is manufactured under the diagnostic doses of medicaments so as to rapidly detect the resistance level to pesticides by the adelphocoris suturalis field population, wherein the medicaments are imidacloprid, endosulfan, cyfluthrin or chlorpyrifos; the diagnostic doses of the medicaments are 824ng/cm<2> of imidacloprid, 20ng/cm<2> of endosulfan, 861ng/cm<2> of cyfluthrin and 7ng/cm<2> of chlorpyrifos. The method provided by the invention is simple and convenient to use; the result is intuitive and obvious; the method is suitable for rapidly detecting the resistance level of adelphocoris suturalis in fields, can be used for overcoming the defects of high sample using quantity, complicity in operation, time consumption and the like by a traditional bioassay method, and can be applied to rapidly detecting the resistance level of the adelphocoris suturalis field population.

The invention discloses a method and a special primer of the method for fast identifying the plutella xylostella ryanodine receptor gene mutation. The invention provides a specific primer combination, which consists of a single chain DNA (deoxyribonucleic acid) molecule shown as a sequence 1 of a sequence table, a single chain DNA molecule shown as a sequence 2 of the sequence table and a single-chain DNA molecule shown as a sequence 3 of the sequence table. The specific primer combination can be used for the following (a) or (b): (a) the specific primer combination can be used for identifying whether plutella xylostella generates the G4899E mutation of the ryanodine receptor gene or not, and the G4899E mutation of the ryanodine receptor gene refers to that an open reading frame of the ryanodine receptor gene is mutated into A from G in the 14696 locus nucleotide of the 5' tail end; and (b) the specific primer combination is used for the auxiliary identification of the resistance of plutella xylostella on bisamide pesticides. The specific primer combination and the application of the specific primer combination have the important significance in the aspects of monitoring the resistance gene frequency of plutella xylostella field populations on the bisamide pesticides and the bisamide pesticide resistance generation and development trend of the plutella xylostella field populations, timely regulating the plutella xylostella preventing and control strategy, guiding the reasonable pesticide use and delaying the resistance development.

The invention provides a method for improving chilo suppressalis control effect by coordinated usage of trap crops and a sex attractant. The method comprises: planting trap crops in lines on ridges of a rice field or a zizania aquatica field, clump distance of the trap crops being 3-4 m, trapping chilo suppressalis female moths to lay eggs on the trap crops; putting the sex attractant in the ice field or the zizania aquatica field, the sex attractant being put in a trap device to trap male insects; parallel distance between the chilo suppressalis sex attractant and the trap crop being 20-25 m. The trap crops and the sex attractant are used in a combined manner in a specific method. The method substantially improves trapping effect of vetiver grass on chilo suppressalis female moths and trapping effect of the sex attractant on the chilo suppressalis male insects, reduces population quantity of chilo suppressalis adults in the rice field (zizania aquatica field) and field population quantity of next generation larva, and improves seedling protection effect on rice (zizania aquatica).

The invention relates to a bradysia odoriphaga collection cage and a collection method of bradysia odoriphaga. The bradysia odoriphaga collection cage comprises a cage body, a screen covering body, a bottom plate light source, a plate, a support, a cloak cover and a power supply. According to the bradysia odoriphaga collection cage and the collection method of the bradysia odoriphaga, by the track of insects climb upwards, the bradysia odoriphaga are induced through different colors of LED lamps, and the bradysia odoriphaga enters the cage body from the 40 mesh screen covering body; other parts of the cage body are covered with a 80 mesh screen, and the included angle between the screen covering body and the support is 90 degrees, so that the bradysia odoriphaga entering the cage body is difficult to escape, and the bradysia odoriphaga attracted to the cage body by a light source falls on the bottom part of the cage body when the light source goes out or physical strength is consumed. According to the invention, a culture dish is arranged on the bottom part of the cage body, so that the bradysia odoriphaga lays eggs conveniently, and an optimum condition is screened to capture the adult; and meanwhile, the field population occurrence rules are researched.

The invention discloses a DNA sequence used for encoding apolygus lucorum vitellogenin. The invention further discloses a specific peptide sequence capable of detecting an AlVg protein expression level by using Western-blot, and the expression difference of AlVg in apolygus lucorum on the protein level is analyzed by utilizing the specificity of the polypeptide. The invention further constructs an atlas of age in days after female adult eclosion and AlVg expression trend. The correlation of AlVg genes and protein expression levels in female adults with single female fecundity is investigated after the female adults are continuously bred on a variety of host plants, in order to construct a related prediction model, which has significant importance on prediction of the spawning potential of the apolygus lucorum. a positive correlation relation between the AlVg expression levels of female apolygus lucorum adults and the single female fecundity after eclosion for 7 days is defined. The specific peptide sequence disclosed by the invention can be used for observing and predicting the population dynamics of the apolygus lucorum in fields, and providing a foundation for research and development of new technology for monitoring field populations of the apolygus lucorum.

The invention belongs to the technical field of pest biological control equipment, and particularly relates to a device and method for stabilizing the number of Trichogramma ostriniae in a field. Theinvention aims to reduce the release cost of Trichogramma, stably raise the population level of Trichogramma in a corn field, continuously reduce the population number of Trichogramma ostriniae in thefield, and finally improve the control effect of Trichogramma. The device comprises a wasp storage device, a connecting ring, a top cover and a control device. The device disclosed by the invention has the advantages that the structure is simple, the releasing cost of Trichogramma can be reduced, the population level of the Trichogramma ostriniae is stably raised, the field population quantity ofthe Trichogramma ostriniae is continuously reduced, and the control effect of the Trichogramma ostriniae is finally improved; the device can maintain the basic quantity of Trichogramma ostriniae in the field for long time, and reduce the hazard level of Trichogramma ostriniae, thereby reducing the number of releases of Trichogramma ostriniae, reducing the prevention and treatment cost and greatlyimproving the efficiency of biological control.

The invention provides a method for improving sesamia inferen control effect by coordinative application of trap crops and a sex attractant. The method comprises: planting trap crops in lines on ridges of a rice field or a zizania aquatica field, clump distance of the trap crops being 3-4 m, trapping sesamia inferen female moths to lay eggs on the trap crops; putting the sex attractant in the ice field (zizania aquatica field), the sex attractant being put in a trap device to trap sesamia inferen male insects; parallel distance between the sesamia inferen sex attractant and the trap crop being 10-15 m. The trap crops and the sex attractant are used in a combined manner in a specific method. The method substantially improves trapping effect of vetiver grass on sesamia inferen female insects and trapping effect of the sex attractant on the sesamia inferen male insects, reduces population quantity of sesamia inferen adults in the rice field (zizania aquatica field) and field population quantity of next generation larva, and improves seedling protection effect on rice (zizania aquatica).

The invention relates to the field of plant protection and particularly provides an indoor biological measuring method of wheat aphids. In the invention, a plastic dropper is employed as a biological measuring tool and is sealed by cotton. The method is simple in operation, can save space and is reduced in damage and error on to-be-test aphids due to operations in a biological measuring process. In addition, wheat seedlings are arranged in the plastic dropper so that the moisture of the wheat seedlings can be maintained well, thereby ensuring accuracy of a biological measuring result. The novel indoor medicine film biological measuring method of the wheat aphids can be used for finding resistance level and distribution thereof of field population of the wheat aphids on medicines in different action mechanisms, thereby guiding field application of the medicines more scientifically.

The present invention discloses a molecular detection method of resistance of diamond back moth against an avermectin target. According to the method, a specific primer designed according a diamond back moth glutamate chloride ion channel gene sequence is used, extracted diamond back moth single-head genomic DNA is used as a template for PCR amplification of glutamate chloride ion channel gene fragments, diamond back moth individual genotypes (sensitive homozygote SS, resistant heterozygote RS and resistant homozygote RR) can be distinguished by analysis of a target fragment purified product direct sequencing chromatogram, and population sample resistance allele mutation frequency can be detected. A detection method of diamond back moth glutamate chloride ion channel gene A309V mutation and a special primer sequence are disclosed, and the method has the advantages of being simple, rapid and high in accuracy, can be used to monitor the occurrence and development trend of allele frequency of the resistance of a diamond back moth field population against avermectin and drug resistance, and provides an important basis for adjustment of diamond back moth chemical control strategies and formulation of measures to delay the development of resistance.

The invention discloses a CAPS marking method for detecting avermectin resistance related gene mutation of spider mites. Spider mite population GluCl3 gene to be detected which contains a gene fragment with G326E mutation is amplified, and enzyme sites are analyzed, wherein a Hinf I enzyme site exists in the amplified fragments of the sensitive population, and the site does not exist in the resistance homozygosis population, amplified fragments containing the Hinf I enzyme site exist in the resistance heterozygosis population, and amplified fragments without the site exist in the resistance heterozygosis population. The method can be used for replacing traditional biological assay methods, and has the advantages of little manpower and few sample amounts, large detection quantity, high sensitivity, good specificity, rapid and simplicity, and accuracy and reliability; the method is suitable for rapid diagnosis of avermectin resistance of Tetranychus urticae field population, and is usedfor carrying out real time monitoring and early warning forecast of avermectin resistance of field spider mites at the same time; the method has important meaning for avermectin resistance monitoringof field spider mites, and application prospect is wide.

The invention provides a method for detecting drug resistance of chilo suppressalis (Walker). According to the method, a drug-carried artificial feed piece is prepared on the basis of diagnosis dosages of an artificial feed drug soaking method, and the drug resistance of field populations of the chilo suppressalis (Walker) is rapidly detected. The drug comprises the following components in diagnosis dosages: 1.502mg/L of abamectin, 38.8334mg/L of monosultap, 20.9033mg/L of triazophos and 20.2148mg/L of chlorantraniliprole. The method provided by the invention is simple and convenient to use, the toxicity and the activity of the drug can be sufficiently reflected, a small amount of insects is needed, the defects that a conventional bioassay method is large in insect amount, tedious to operate, great in time and labor and the like are overcome, and the drug resistance of the field populations of the chilo suppressalis (Walker) can be rapidly detected.

The invention discloses a Pea seed selection method, relates to a plant heterosis utilization method, and belongs to the technical field of the agriculture. The method is characterized in that the composition is {[(spotted colored pea*white jade tendril pea)F2+radiation]F2*American needle-leaf pea}F1*{[(Beijing No.5*Donough)F2+radiation]F2*Zhongwan No.5}F1. According to the invention, Guwan No.1 is half-leafless pea, and a strong lodging-resistant population can be formed through the mutual winding and drawing of the developed tendrils in plants; the concentrated florescence and the fast maturation yellowing create conditions for the irrigated land crop rotation, so it is benefit for drug spray for disease and pest control; pinnately compound leaves protrude to form the tendrils, so the ventilation and light transmission condition in the field populations are good, thereby it is benefit for the drug spray in the growth stage for the disease and pest control, and the mechanical operation is suitable; and good grain quality, good disease resistance, good comprehensive agronomic properties, large yield increase potential, strong lodging resistance, and suitableness for irrigated land plantation are reached.

The invention discloses a molecular detection method and special primer for resistance of sesamia inferens to chlorantraniliprole, and provides a special primer composition which is composed of single-chain DNA molecules shown in the sequence 1, the sequence 2 and the sequence 3 in the sequence table. The special primer composition can be used for detecting whether gene G4924E mutation happens to an acting site, namely, a ryanodine receptor, of chlorantraniliprole or not. The gene G4924E mutation of the ryanodine receptor of sesamia inferens means that the 14771th nucleotide at the 5' end of an open reading frame of the ryanodine receptor gene mutates into A from G, and therefore resistance of sesamia inferens to chlorantraniliprole is detected. The special primer composition and application of the special primer composition have important significance in monitoring development trends of resistance of sesamia inferens field populations to chlorantraniliprole, guiding reasonable pesticide utilization, delaying resistance development, formulating resistance government policies and regulating prevention and control strategies in time.

The invention provides a method for detecting the insecticide resistance of apolygus lucorum. Under the diagnostic dose of an insecticide, a diagnostic glass tube is manufactured for rapidly detecting the resistance level of an apolygus lucorum field population to the insecticide, wherein the insecticide is malathion, chlorpyrifos, methomyl, cyhalothrin, imidacloprid or endosulfan; the diagnostic dose of the insecticide as follows: 67 ng/cm<2> of malathion, 560 ng/cm<2> of chlorpyrifos, 620 ng/cm<2> of methomyl, 3,046 ng/cm<2> of cyhalothrin, 886 ng/cm<2> of imidacloprid and 1,092 ng/cm<2> of endosulfan. The method disclosed by the invention is convenient to use and is suitable for rapidly detecting the resistance level of the apolygus lucorum in the field; results are intuitive and clear; the disadvantages that the conventional bioassay method has a large number of samples, tedious operation, time waste and the like are overcome; the method can be used for rapidly detecting the resistance level of the apolygus lucorum field population.

The invention relates to the technical field of crop cultivation and pest control, in particular to a crop ecological cultivation method, including a cultivation mode and pest control; the cultivationmode refers to planting susceptible variety of crop at the edge of a field block and planting the susceptible variety and conventional variety alternatively in the field; the pest control refers to taking prevention measures to trap and kill pests intensively in the area planted with the susceptible variety according to the prevention threshold of crop pests, while taking no prevention measures in the area planted with conventional variety. The cultivation mode realizes local concentration of pests in the field, and further realizes trapping and killing the pests by applying insecticide in the pest concentrated area, thereby effectively suppressing field population density of the pests, achieving the purpose of controlling pests with less insecticide, relieving the problem of pest resistance to insecticide and reducing Bt-resistant ostrinia nubilalis. The crop ecological cultivation method is an effective ecological prevention and control mode integrated with culture and plant protection.

An automated method for calibrating in real time a diagnostic analyzer includes the steps of receiving diagnostic results of patient samples calculated by the analyzer using pre-set parameters of the analyzer, and calculating the centroid of the analyzer's diagnostic results. The centroid of the analyzer's diagnostic results is subtracted from a centroid of diagnostic results of a field population of comparable analyzers to obtain a bias in the centroid of the analyzer's diagnostic results. The bias is compared to predetermined limits Should the bias fall outside the predetermined limits, an optimization factor is derived and applied to the analyzer's diagnostic results to obtain optimized results from the analyzer without changing the pre-set parameters of the analyzer.

The invention provides a method for measuring the pesticide resistance of macrosiphum avenae. The method comprises the following steps of manufacturing a diagnosis glass pipe under the diagnosis dosage of a pesticide, rapidly detecting the resistance level to the pesticide from macrosiphum avenae field population, wherein the pesticide is imidacloprid, pirimor, omethoate or beta-cypermethrin, and the diagnosis dosage of the pesticide comprises 4289ng/cm<2> of the imidacloprid, 103ng/cm<2> of the pirimor, 224ng/cm<2> of the omethoate, and the 4695ng/cm<2> of the beta-cypermethrin. According to the method provided by the invention, the use is simple and convenient, the result is intuitive, the method is suitable for rapidly detecting macrosiphum avenae resistance level in the field, the disadvantages that the number of samples in the traditional detecting method is large, the operation is tedious, the time is wasted and the like are overcome, and the method is used for rapidly detecting the macrosiphum avenae field resistance level.

The invention discloses a molecular detection method for CrylAc toxin non-recessive resistance cadherin cytomere deletion mutation of cotton bollworm. The detection method comprises the following three steps: 1, extracting a genome DNA of a cotton bollworm sample respectively; 2, performing polymerase chain reaction (PCR) amplification on a specific cadherin cytomere segment; and 3, performing agarose gel electrophoresis on the PCR product, and precisely identifying the genotype according to an electrophoretogram to determine the resistance gene frequency of different population. According to the method, no test bollworm needs to raise, so that labor force and resources are saved; and the genotype of the cotton bollworm can be detected quickly and precisely, a single bollworm test only needs several hours, the collection and identification for the frequency of resistant individuals of field population only need several weeks, and the identification result can timely provide correct guide for the effective resistance management of the cotton bollworm.

The invention provides a method for detecting insecticide resistance of green peach aphids. The method includes under a diagnostic dose, adopting a microplate reader kinetic method to detect resistance of field population individuals of the green peach aphids to organophosphorus or carbamate insecticide, wherein the organophosphorus insecticide is omethoate whose diagnostic dose is 180.43 muM, and the carbamate insecticide is pirimicarb whose diagnostic dose is 18.85 muM.