Sperm cryopreservation method of scatophagus argus

A cryopreservation and money fish technology, which is applied in the preservation, application, and animal husbandry of human or animal bodies, can solve the problems of rare long-term preservation of money fish sperm, increase the difficulty of artificial reproduction, and asynchronous development of male and female gonads. The reproductive system is fully developed, time-saving, and the effect of a sound mechanism

Inactive Publication Date: 2020-02-14
GUANGXI ACADEMY OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because the breeding of money fish is seasonal, and there is a phenomenon that the development of male and female gonads is not synchronized, and males generally mature before females, these all increase the difficulty of artificial reproduction.
If the sperm of money fish can be collected and stored for a long time during the breeding season, these difficulties can be overcome, thereby meeting the breeding and production needs of money fish throughout the year, and improving the artificial insemination efficiency and seedling survival rate of money fish in non-breeding seasons, but at present There are few ways to preserve money fish sperm for a long time

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A method for cryopreservation of money fish sperm, characterized in that: comprising the steps of:

[0023] (1) Semen collection: select 2-year-old money fish with a weight of 200g, inject 150IU / kg of human chorionic gonadotropin into the back muscle, induce labor at a water temperature of 27-28°C for 20 hours, and observe the changes in the semen volume of the male fish during this period , and 0.5ml of semen can be sampled at intervals of 2 to 3 hours, and the parameters such as sperm motility and lifespan can be measured according to conventional methods. Gently squeeze the abdomen until the milky white semen flows out from the genital opening, draw the semen with a syringe to prevent the mixing of urine, blood and water, and inject the collected semen into the cryopreservation tube;

[0024] (2) Semen dilution: first mix and dilute the semen collected in step (1) with the antifreeze solution at a volume ratio of 1:1 to obtain a preservation solution, and the antifre...

Embodiment 2

[0029] A method for cryopreservation of money fish sperm, characterized in that: comprising the steps of:

[0030] (1) Semen collection: select 2-year-old money fish weighing 210g, inject 150IU / kg of human chorionic gonadotropin into the back muscle, induce labor at a water temperature of 27-28°C for 24 hours, and observe the changes in the semen volume of the male fish during this period , and 0.5ml of semen can be sampled at intervals of 2 to 3 hours, and the parameters such as sperm motility and longevity can be measured according to conventional methods. Squeeze the abdomen gently until milky white semen flows out from the genital opening, draw the semen with a syringe to prevent the mixing of urine, blood and water, and inject the collected semen into the cryopreservation tube;

[0031] (2) Semen dilution: first mix and dilute the semen collected in step (1) with the antifreeze solution at a volume ratio of 1:1 to obtain a preservation solution, and the antifreeze solutio...

Embodiment 3

[0036] A method for cryopreservation of money fish sperm, characterized in that: comprising the steps of:

[0037] (1) Semen collection: select 2-year-old money fish weighing 220g, inject 150IU / kg human chorionic gonadotropin into the back muscle, induce labor at a water temperature of 27-28°C for 36 hours, and observe the changes in the semen volume of the male fish during this period , and 0.5ml of semen can be sampled at intervals of 2 to 3 hours, and the parameters such as sperm motility and longevity can be measured according to conventional methods. Squeeze the abdomen gently until milky white semen flows out from the genital opening, draw the semen with a syringe to prevent the mixing of urine, blood and water, and inject the collected semen into the cryopreservation tube;

[0038] (2) Semen dilution: first mix and dilute the semen collected in step (1) with the antifreeze solution at a volume ratio of 1:1 to obtain a preservation solution, and the antifreeze solution i...

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PUM

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Abstract

The invention discloses a sperm cryopreservation method of scatophagus argus. The sperm cryopreservation method comprises four steps of sperm collection, sperm dilution, sperm cryopreservation and sperm thawing, firstly male scatophagus argus is selected to inject human chorionic gonadotropin into dorsal muscle, then the sperm of the scatophagus argus is collected by an artificial abdominal extrusion method, then the sperm and antifreezing liquid are mixed to obtain preservation liquid of the scatophagus argus sperm, a diluent is prepared by mixing any one of a TS-2 diluent, a TS-19 diluent, aHank's solution, a Cortland diluent and sterilized seawater with dimethylsulfoxide; the sperm preservation liquid is injected into a cryopreservation tube, then freezing is conducted by adopting a step-by-step cooling method, and then the cryopreservation tube is immersed in liquid nitrogen for long-term preservation; when taking for use, the cryopreservation tube containing the sperm preservation liquid is taken out from the liquid nitrogen, and then is placed in a water bath with the temperature of 42 DEG C. According to the sperm cryopreservation method, the sperm of the scatophagus arguscan be preserved for a long time, and the sperm survival rate and insemination rate after thawing and resuscitation are high.

Description

technical field [0001] The invention belongs to the technical field of biological low-temperature cryopreservation, and in particular relates to a method for cryopreservation of money fish sperm. Background technique [0002] The South China Sea is the third continental marginal sea in the world, with an area of ​​about 3.5 million km 2 , of which the sea area under the jurisdiction of our country is about 2.01 million km 2 . Due to its vast sea area, excellent water quality, and rich marine resources, there are many species and varieties of marine organisms such as fish, shrimp, shellfish, and algae. There are a total of about 4,168 species, accounting for about 52.2% of the corresponding species in the country. With the acceleration of the global economy and the competition among countries around the South China Sea to develop the marine economy, my country has also accelerated the pace of development of marine resources in the South China Sea. However, with the acceler...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0221A01N1/0226
Inventor 彭敏陈秀荔杨春玲曾地刚
Owner GUANGXI ACADEMY OF FISHERY SCI
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