52 results about "HCG - Human chorionic gonadotropin" patented technology

The invention discloses a preparation method of a multifunctional test paper for early pregnancy. The preparation method comprises the steps: preparing a nitrocellulose membrane; labeling and curing a polyester fiber strip; and assembling and cutting. A human chorionic gonadotropin beta core segment and a human chorionic gonadotropin regular molecule in urine are detected by applying a principle of a double-antibody sandwich method and an immunochromatographic method, detection steps are simple, a result can be observed within 5 min, and a common user can detect the result, and the detection result is more accurate. Through comparing color depth of a first detection line and a second detection line, whether to be pregnant and whether to be normally pregnant are determined, and ectopic gestation and threatened abortion are subjected to risk assessment.

The invention discloses hyperglycosylated human growth hormone fusion protein. The human growth hormone fusion protein sequentially contains a human growth hormone (hGH), a flexible peptide joint (L), human chorionic gonadotropin beta-carboxyl terminal rigid peptide (CTP) and a human immunoglobulin Fc fragment from the N terminal to the C terminal. The invention further discloses a method for efficiently preparing the fusion protein. Compared with a recombinant hGH, the built fusion protein has more excellent in-vivo drug efficacy, the in-vivo circulation half-life period is prolonged, the administration frequency is greatly decreased, and the bioavailability is improved; meanwhile, the production process is simpler and more efficient.

The invention relates to a detection kit for hyperglycosylated modification of an hCG (human chorionic gonadotropin) tumor marker. According to the detection kit, magnetic beads are combined with an antibody (MCA-A) to serve as a capture antibody to capture total hCG in a to-be-detected sample, wherein the affinity of the antibody (MCA-A) is least influenced by glycosylation change; an antibody (MCA-B) most influenced by glycosylation change and another antibody (MCA-C) less influenced by glycosylation are used for detecting the total protein content and the N-glycosylation modification degree of hCG in the sample simultaneously, so that a tumor biomarker is identified. The detection kit improves the accuracy, the sensitivity and the specificity of an immunodiagnosis method greatly and has great practical value for detection of hyperglycosylated modification of the tumor marker in the actual sample.

The invention provides a hormone kit for scatophagus argus spawning induction and an artificial spawning induction and insemination method for obtaining scatophagus argus fertilized eggs in batches, which belong to the technical field of aquaculture. The hormone kit for scatophagus argus spawning induction comprises gonadotropin releasing element A4, doxolone maleate and human chorionic gonadotropin which are independently subpackaged. Based on the hormone kit, scatophagus argus is subjected to artificial spawning induction by adopting a three-needle method, so that the problems of long effecttime, female scatophagus argus stagnation, high difficulty in artificial spawn extrusion after spawning induction, egg mass and gonad degeneration are effectively solved. The artificial inseminationmethod is adopted, and the problems that natural spawning eggs of scatophagus argus are over-mature or collapse in vivo, the influence of the environment is large, the spawning induction success rate,the fertilization rate and the hatching rate are extremely low, and fertilized eggs are difficult to obtain in batches are effectively solved.

The invention discloses a micro-fluidic chemiluminescence detecting system for magnetic particles for detecting gonad series. The system comprises a bottom plate and an upper chip, and the upper chipcomprises a sample adding area in the center of the upper chip and seven micro-fluidic reaction detecting channels communicated with the sample adding area, wherein the seven micro-fluidic reaction detecting channels include a testosterone micro-fluidic reaction detecting channel, a progesterone micro-fluidic reaction detecting channel, an estradiol micro-fluidic reaction detecting channel, a follicle-stimulating hormone micro-fluidic reaction detecting channel, a luteinizing hormone micro-fluidic reaction detecting channel, a prolactin micro-fluidic reaction detecting channel and a beta-humanchorionic gonadotropin micro-fluidic reaction detecting channel. In application of the system, the bottom plate is arranged under the upper chip, and magnets which are permanent magnets or electromagnets are arranged at positions, corresponding to magnetic particle coating areas of the micro-fluidic reaction detecting channels, of the bottom plate.

The invention refers to human chorion gonadotropic hormone (hCG) chimera peptide CP1, ramification CP10, and the making method by gene engineering. They have 6 amino (N) ends or 5 continuous broad-spectrum or single strong T-cell table, and 3 B-cell tables of hCG beta-antigen at carboxylic end: beta5 45-52, beta9 113-116 and beta8 137-144. The genes coding CP1 and CP10 can all be expressed in colibacillus, and the expressed product can be obtained by the SDS-polyacrylamide gelatin electrophoresis (SDS-PAGE).

The invention discloses a human chorionic gonadotropin detection kit, a preparation and an application method thereof. The kit comprises a calibrator, a quality control serum, an anti-reagent A, an anti-reagent B, a magnetic particle reagent and a luminescent substrate. The calibrator and the quality control serum are prepared by first dissolving a Total-[beta]hCG antigen with a protein-containingbuffer solution, and then diluting the protein-containing buffer solution to a fresh bovine serum-containing buffer solution; the anti-reagent A is a Total-[beta]hCG coating antibody labeled by a fluorescein isothiocyanate, and the anti-reagent B is a Total-[beta]hCG labeled antibody labeled by a alkaline phosphatase; the magnetic particle reagent is prepared by coupling the fluorescein isothiocyanate antibody with magnetic particles. The invention also relates to a preparation method of the kit and a method for quantitatively detecting the content of human chorionic gonadotropin (Total-[beta]hCG) in human serum by using the kit. According to the human chorionic gonadotropin detection kit, the preparation and the application method thereof, the kit is reliable in performance, high in sensitivity, wide in linear range, and can be used in conjunction with semi-automatic and full-automatic instruments.

The invention discloses a novel method for determining human chorionic gonadotropin. The method comprises the following steps of: by taking waste biomass papaya peel as a raw material, synthesizing blue high-fluorescence carbon quantum dots in one step by adopting a hydrothermal method, and combining the blue high-fluorescence carbon quantum dots with silver ions to form AgCQDs; and synthesizing PS through photolysis and coordination polymerization of ferrocene dicarboxylic acid molecules in methyl alcohol, and combining AgCQDs with negative electricity and PS modified by PEI to be changed into PS with positive electricity through electrostatic attraction, wherein the obtained composite material and an rGO@Ag composite material jointly prepare a sandwich type immunosensor for HCG detection. The method is high in sensitivity and good in stability.

The embodiment of the invention provides a drying device for extracting human chorionic gonadotropin from human urine and an extraction method, and relates to the technical field of biological pharmacy. The drying device for extracting human chorionic gonadotropin from human urine comprises a heating storage mechanism and a centrifugal drying mechanism. The heating storage mechanism comprises an outer tank body, a bottom plate, a top cover, a first partition plate, a second partition plate and an electric heater, the top cover is hinged to the top of the outer tank body, the bottom plate is fixedly arranged at the bottom of the outer tank body, and the first partition plate and the second partition plate are fixedly connected to the lower portion of the interior of the outer tank body; and the first partition plate is positioned above the second partition plate. When the electric heater heats and dries the interior of the outer tank body, the driving assembly drives the shaft rod to rotate, and the rotating shaft rod drives the supporting frame piece and the filter element cylinder on the rotating shaft rod to quickly rotate, so that residual water in precipitates is quickly thrown out; and the device is matched with an electric heater to quickly dry the precipitate.

The invention discloses a method for inducing estrus and ovulation of a female cat, and belongs to the technical field of animal artificial propagation. The invention provides the method for inducingestrus synchronization and superovulation of the female cat in order to solve the problem that estrus and ovulation are difficult to synchronize in artificial propagation of the female cat. The methodspecifically comprises the following steps: continuously feeding adult healthy female cats with altrenogest with a dosage of 0.01-20mg/cat/day for 10-20 days, stopping feeding the altrenogest for 2 to 3 days, injecting 10-1000IU of pregnant mare serum hormone into the muscle of the female cat, and intramuscularly injecting 10-1000 IU of human chorionic gonadotropin after 72-96 hours, so that thefemale cat can perform estrus synchronization and superovulation within a preset time. The key technical difficulty of artificial breeding of the domestic cat or the feline is solved.

The invention discloses a sperm cryopreservation method of scatophagus argus. The sperm cryopreservation method comprises four steps of sperm collection, sperm dilution, sperm cryopreservation and sperm thawing, firstly male scatophagus argus is selected to inject human chorionic gonadotropin into dorsal muscle, then the sperm of the scatophagus argus is collected by an artificial abdominal extrusion method, then the sperm and antifreezing liquid are mixed to obtain preservation liquid of the scatophagus argus sperm, a diluent is prepared by mixing any one of a TS-2 diluent, a TS-19 diluent, aHank's solution, a Cortland diluent and sterilized seawater with dimethylsulfoxide; the sperm preservation liquid is injected into a cryopreservation tube, then freezing is conducted by adopting a step-by-step cooling method, and then the cryopreservation tube is immersed in liquid nitrogen for long-term preservation; when taking for use, the cryopreservation tube containing the sperm preservation liquid is taken out from the liquid nitrogen, and then is placed in a water bath with the temperature of 42 DEG C. According to the sperm cryopreservation method, the sperm of the scatophagus arguscan be preserved for a long time, and the sperm survival rate and insemination rate after thawing and resuscitation are high.

The invention relates to the field of field of fluorescence immunochromatography, and concretely relates to a beta-human chorionic gonadotrophin near-infrared fluorescence detection reagent card, a kit and a purpose thereof. The beta-human chorionic gonadotrophin near-infrared fluorescence detection reagent card comprises a sampling gasket, a glass cellulose membrane, a cellulose nitrate membrane,and a water-absorption gasket arranged in order; wherein, the glass cellulose membrane contains a fluorescence probe-first anti-beta human chorionic gonadotrophin antibody and a fluorescence probe-test antigen; and a first test line, a second test line and a control line are arranged on the cellulose nitrate membrane in order; the first test line is coated with a second anti-beta human chorionicgonadotrophin antibody; the second test line is coated with the beta-human chorionic gonadotrophin antigen; and the control line is coated with the antibody of the test antigen.

There is disclosed a device for pregnancy detection is provided, the device comprising: a central main body comprising a first end and a second end; a pregnancy detection element that is mounted at an edge of the second opening, and an upper longitudinal crease and a lower longitudinal crease, wherein the device is opened by applying simultaneous pressure to the upper longitudinal crease and the lower longitudinal crease, wherein, when in operation, the first opening of the first end is configured to receive urine from a female user while the female user is in a standing position and enables the urine to pass through the pregnancy detection element, and wherein, when the urine comes in contact with the pregnancy detection element, the pregnancy detection element immunologically detects human chorionic gonadotropin (hCG) present in the urine of the female user and indicates positive pregnancy result if the female user is pregnant.

The present invention provides novel antibody vaccine conjugates and methods of using them to induce cytotoxic T cell (CTL) responses. In a specific embodiment, the vaccine conjugate comprises human chorionic gonadotropin beta subunit (beta hCG) antigen linked to an anti-mannose receptor (MR) antibody.

The invention discloses a using and preparation methods of a human chorionic gonadotropin test strip for a full-automatic analyzer. The preparation method comprises the following steps: adhering and fixing a coating film to a PVC rubber plate; sequentially assembling a sample pad, a colloidal gold combination pad and a water absorption material which are configured according to the requirements onto the PVC rubber plate with the coating film; adhering a protective film adhesive tape containing a warning line on the sample pad according to the requirements; adhering a protective film adhesive tape containing a warning line to the water absorption paper strip; carrying out cutting to obtain a test strip; testing the lowest detection limit of the test strip; and carrying out positive/negativespecificity analysis with high-concentration hLH, hFSH, hLH and hTSH samples. According to the using method, about 60[mu]L of a sample urine, serum or plasma sample is added to a sample pad area of the test strip by using a full-automatic analyzer sample adding needle or manually using a dropper or a dropper/pipette. Positions of the detection line and the quality control line are relatively fixed, so that full-automatic instrument result interpretation is facilitated, the clinical application value is achieved, and the inspection efficiency can be greatly improved.

The invention discloses an anti-interference photoelectrochemical biosensor as well as a preparation method and application thereof, and belongs to the technical field of biosensors. The photoelectrochemical biosensor is used for detecting human chorionic gonadotropin hCG, and the prepared photoelectrochemical biosensor has excellent performance of resisting reducing small molecules and biomacromolecule interference detection signals at the same time. The anti-interference photoelectrochemical biosensor is constructed by sequentially modifying hCG recognition polypeptide and antifouling polypeptide on a photocathode, and photocurrent signal detection is realized by utilizing the hindering effect of the obvious steric hindrance effect of the hCG on charge transfer of the sensor. The anti-interference photoelectrochemical biosensor constructed on the basis of the polypeptide is simple and convenient in preparation process, has the application potential of accurately and sensitively detecting the hCG in an actual biological sample, and is suitable for market popularization and application.

The invention discloses a magnetic particle direct chemiluminiscence kit for detecting the content of free beta-hCG (human chorionic gonadotropin) and a use method of the magnetic particle direct chemiluminiscence kit. The kit comprises magnetic microspheres coated with streptavidin, a biotin-labeled beta-hCG antibody, a beta-hCG standard substance, an acridinium ester-labeled antibody with different beta hCG recognition sites, a washing buffer solution, an acridinium ester labeled antibody diluent, a standard substance diluent, an excitation solution A and an excitation solution B containing1%-2% of Triton X100 and ethanol. According to the kit, the magnetic microsphere and streptavidin protein coupling technology, the biotin antibody labeling technology and the acridinium ester antibodylabeling technology are combined, and particularly components and proportions of the excitation solutions are debugged and improved, and therefore, the kit has various characteristics of high sensitivity, strong specificity, high accuracy and high repeatability. The kit can be used for detecting the direct chemiluminiscence performance of magnetic particles and better detecting the content of free beta-hCG (human chorionic gonadotropin).

The invention discloses a kit as well as a preparation method and application thereof, and belongs to the technical field of drug detection. The kit is used for detecting the content of beta human chorionic gonadotropin. The kit comprises an R1 reagent, an R2 reagent and an R3 reagent; the R1 reagent comprises streptavidin-coated magnetic beads and a first buffer solution with a pH value of 7.2-7.6; the R2 reagent comprises an enzyme labeled beta human chorionic gonadotropin monoclonal antibody and a second buffer solution with the pH value of 6.3-6.7; and the R3 reagent comprises a biotin labeled beta human chorionic gonadotropin monoclonal antibody and a third buffer solution with the pH value of 7.2-7.6. When the kit provided by the invention is used for detecting the content of beta human chorionic gonadotropin, the sensitivity is high, and the stability and the repeatability are good.

The invention relates to the technical field of peptide aptamers, in particular to a human chorionic gonadotropin peptide aptamer. The aptamer comprises any one of the following polypeptide sequences: P1, MHLMRMKPLLLT; P2, MHPRKMLQLMLN; P3, STRLRRRSRRQT; P4, PPLRINRHILTR; P5, MKLKPMRIMINP; and P6, MKSRMLPLNRRL. The invention also provides a preparation method of the human chorionic gonadotropin peptide aptamer. The human chorionic gonadotropin peptide aptamer provided by the invention is good in HCG binding specificity and high in binding rate, and can more accurately reflect the HCG condition of a sample when being applied to HCG detection. The application of the human chorionic gonadotropin peptide aptamer includes but is not limited to manufacturing of a detection reagent for detecting HCG, manufacturing of a biological detection sensor for detecting HCG and the like.

The invention discloses a detection kit for a free human chorionic gonadotropin beta subunit. The detection kit comprises a biotin-labeled free human chorionic gonadotropin beta subunit antibody, a streptavidin magnetic particle suspension, an acridinium ester-labeled free human chorionic gonadotropin beta subunit antibody, a standard substance, a cleaning solution, a diluent, a chemiluminescent substrate solution A and a chemiluminescent substrate solution B. The streptavidin and the biotin have high-specificity binding capacity, streptavidin and biotin-labeled high-purity antibody non-covalent bonds are specifically bound, the cascade amplification effect is achieved and the reaction is highly specific. The high specificity of antibody-antigen reaction and high sensitivity of acridiniumester luminescence are combined, photons generated by reaction are captured by acridinium ester to detect the concentration of the product, and the detection kit has the characteristics of higher sensitivity, short reaction time, simple operation and high anti-interference performance.