In-vitro culture solution of immature oocytes, and preparation method and application of in-vitro culture solution
An in vitro culture, oocyte technology, applied in the field of assisted reproduction, to restore meiosis, improve the rate of high-quality embryos, and improve immunity
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[0056] 2. The preparation method of in vitro culture fluid in embodiment 1~5, comprises the following steps:
[0057] S1. prepare solution I, comprise the following steps:
[0058] 1) Make the required utensils for preparation go through dry heat at 250°C for 30 minutes to remove the heat source;
[0059] 2) Under 100-grade conditions, weigh calcium chloride, potassium chloride, magnesium sulfate, sodium chloride, sodium bicarbonate, sodium dihydrogen phosphate hydrate, arginine, cystine, cysteine, Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Threonine, Tryptophan, Tyrosine, Valine, Asparagine, Aspartame amino acid, glutamic acid, N(2)-L-alanyl-L-glutamine, glycine, proline, serine, calcium D-pantothenate (B5), choline chloride, folic acid (B9), Inositol (B8), Niacinamide (B3), Pyridoxal Hydrochloride (B6), Riboflavin (B2), Thiamine (B1), Ascorbic Acid (C), D-Glucose, Sodium Pyruvate, Sodium Lactate and Chin Damycin, and add water and mix uniformly to ...
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