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Anti-interference photoelectrochemical biosensor as well as preparation method and application thereof

A biosensor and photoelectrochemical technology, applied in the field of biosensors, can solve the problems of limited detection sensitivity, large sample consumption, and time-consuming operation, and achieve the effects of convenient operation, simple detection operation, and low cost

Active Publication Date: 2020-12-22
QINGDAO UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these hCG detection methods have made great progress, there are still some defects, such as expensive, time-consuming operation, large sample consumption, limited detection sensitivity, etc.

Method used

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  • Anti-interference photoelectrochemical biosensor as well as preparation method and application thereof
  • Anti-interference photoelectrochemical biosensor as well as preparation method and application thereof
  • Anti-interference photoelectrochemical biosensor as well as preparation method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Because of CuBi 2 o 4 The photocurrent response value of the / Au photocathode has a significant impact on the detection sensitivity of the finally prepared anti-interference photoelectrochemical biosensor, so the following for CuBi 2 o 4 / Au photocathode preparation process parameters were optimized:

[0058] 1) Because of CuBi 2 o 4 The amount of deposition on the electrode can be reflected by the deposition time, so for CuBi 2 o 4 The deposition time is optimized as follows:

[0059] CuBi 2 o 4 The preparation of the nanometer film on the ITO electrode was deposited by the constant potential method, and the ethylene glycol solution was used as the electrolyte, which contained 100mM Bi(NO 3 ) 3 and 30mM Cu(NO 3 ) 2 , the deposition process is at a constant potential E=-1.8V vs Hg / Hg 2 Cl 2 , and the duration is selected as 20s, 40s, 60s, 80s, 100s respectively; then after calcination in air atmosphere at 450°C for 3h, CuBi 2 o 4 Modified electrodes.

...

Embodiment 2

[0065] Because the amount of modification of the hCG recognition polypeptide on the sensing electrode has a significant impact on the quantitative detection range of the anti-interference photoelectrochemical biosensor, the process parameters for modifying the hCG recognition polypeptide are optimized:

[0066] And because the amount of modification of the hCG recognition polypeptide on the sensing electrode can be reflected by its incubation concentration on the electrode, the incubation concentration of the hCG recognition polypeptide is optimized as follows:

[0067] By optimizing the CuBi 2 o 4 Add 20μL of hCG recognition peptide solutions with concentrations of 0.05mg / mL, 0.1mg / mL, 0.2mg / mL, 0.3mg / mL, and 0.4mg / mL to the Au photocathode dropwise, incubate in a refrigerator at 4°C for 12h, and wash with phosphate After washing the electrode with buffer solution (pH 7.4, 10 mM), the hCG recognition polypeptide modified photocathode is obtained.

[0068] It can be obtained...

experiment example 1

[0074] (1) CuBi 2 o 4The preparation steps of the nano-membrane modified electrode are as follows:

[0075] CuBi 2 o 4 The nano-membrane has a modified area of ​​0.25cm 2 The preparation of indium tin oxide (ITO) electrodes was deposited by a potentiostatic method, specifically using ethylene glycol solution as the electrolyte, which contained 100mM Bi(NO 3 ) 3 and 30mM Cu(NO 3 ) 2 , the deposition process is at a constant potential E=-1.8V vs Hg / Hg 2 Cl 2 , and the duration is 60s; after calcination at 450°C in air atmosphere for 3h, CuBi 2 o 4 Nanofilm modified electrodes.

[0076] Scanning Electron Microscope as attached Figure 4 Shown, CuBi 2 o 4 The nanomembrane is an internal interconnected structure composed of a large number of smooth particles with a size of 90-120nm, and has a large specific surface area, which is conducive to the subsequent in-situ growth of Au nanoparticles.

[0077] (2) CuBi 2 o 4 / Au photocathode preparation steps are as follows...

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Abstract

The invention discloses an anti-interference photoelectrochemical biosensor as well as a preparation method and application thereof, and belongs to the technical field of biosensors. The photoelectrochemical biosensor is used for detecting human chorionic gonadotropin hCG, and the prepared photoelectrochemical biosensor has excellent performance of resisting reducing small molecules and biomacromolecule interference detection signals at the same time. The anti-interference photoelectrochemical biosensor is constructed by sequentially modifying hCG recognition polypeptide and antifouling polypeptide on a photocathode, and photocurrent signal detection is realized by utilizing the hindering effect of the obvious steric hindrance effect of the hCG on charge transfer of the sensor. The anti-interference photoelectrochemical biosensor constructed on the basis of the polypeptide is simple and convenient in preparation process, has the application potential of accurately and sensitively detecting the hCG in an actual biological sample, and is suitable for market popularization and application.

Description

technical field [0001] The invention belongs to the technical field of biosensors and relates to a method and strategy for detecting human chorionic gonadotropin. More specifically, it relates to an anti-interference photoelectrochemical biosensor constructed based on a recognition polypeptide and an antifouling polypeptide and a preparation method thereof. Background technique [0002] Photoelectrochemical biosensing is a new generation of sensing technology developed by the organic combination of photoelectrochemical technology and electrochemical analysis method. It not only inherits the advantages of simple device, convenient operation, low cost, easy integration and miniaturization of electrochemical biosensing, but also makes the background interference low; and the system can realize self-supply, which is easier for real-time and rapid on-site detection. [0003] Photoelectrochemical biosensing can be divided into two types: anodic sensing and cathodic sensing. Alth...

Claims

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Application Information

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IPC IPC(8): G01N27/26G01N27/42G01N27/30
CPCG01N27/26G01N27/42G01N27/301
Inventor 范高超顾士亭胡泽罗细亮
Owner QINGDAO UNIV OF SCI & TECH
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