Preparation method of multifunctional test paper for early pregnancy

A detection test strip and a multifunctional technology are applied in the field of preparation of multifunctional detection test strips for early pregnancy, which can solve problems such as complicated operations, and achieve the effects of simple detection steps and accurate detection results.

Active Publication Date: 2014-05-07
NANTONG EGENS BIOTECH
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  • Abstract
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  • Claims
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Problems solved by technology

[0004] At present, the number of days of pregnancy or the detection of ectopic pregnancy are detected by blood test, ultrasound detection, HCG detection and ultrasound

Method used

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  • Preparation method of multifunctional test paper for early pregnancy

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Embodiment 1

[0052] Randomly sample from the same batch, the minimum sampling amount shall not be less than 3 times of the testing amount.

[0053] After sampling, put the product to be inspected in the yellow waiting area. If it can be inspected on the same day, it can be inspected on the same day. If it cannot be inspected, it must be inspected on the next day. The remaining paper strips that pass the inspection can be used as reserved samples, temporarily placed in the qualified product area, and then a certain amount is taken from production to reserve samples of finished products according to the output and sample requirements.

[0054] 1. The required reagents are as follows: 0mIU / ml sample solution: protein-containing phosphate buffered saline (PBS) is 0mIU / ml HCG sample solution. 10ng / ml, 5ng / ml, 2ng / mll human chorionic gonadotropin beta fragment (β-HCG) sample solution: Dilute human chorionic gonadotropin beta fragment (β-HCG) standard with phosphate buffer ( 0.01M PBS) to the co...

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Abstract

The invention discloses a preparation method of a multifunctional test paper for early pregnancy. The preparation method comprises the steps: preparing a nitrocellulose membrane; labeling and curing a polyester fiber strip; and assembling and cutting. A human chorionic gonadotropin beta core segment and a human chorionic gonadotropin regular molecule in urine are detected by applying a principle of a double-antibody sandwich method and an immunochromatographic method, detection steps are simple, a result can be observed within 5 min, and a common user can detect the result, and the detection result is more accurate. Through comparing color depth of a first detection line and a second detection line, whether to be pregnant and whether to be normally pregnant are determined, and ectopic gestation and threatened abortion are subjected to risk assessment.

Description

technical field [0001] The invention belongs to the field of medical testing reagents, and relates to a preparation method of a multifunctional early pregnancy detection test paper. Background technique [0002] During early pregnancy, the number of days of pregnancy is calculated from the first day after the end of the last menstrual period. This calculation method is relatively inaccurate; the general early pregnancy test paper is detected by a double-antibody sandwich one-step method, with colloidal gold as an indicator Marking, detecting the concentration of HCG in urine can only be used to judge whether you are pregnant, but cannot detect the time of pregnancy and whether it is ectopic pregnancy. Under normal circumstances, the fertilized egg will migrate from the fallopian tube to the uterine cavity, and then settle down and slowly develop into a fetus. However, due to various reasons, the fertilized egg went wrong during the migration process and did not reach the ut...

Claims

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Application Information

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IPC IPC(8): G01N33/558G01N33/531G01N33/76
CPCG01N33/558G01N33/76G01N2333/59G01N2800/368
Inventor 欧卫军
Owner NANTONG EGENS BIOTECH
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