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Synthetic chimeric peptide of human chorionic gonadotrophin genetic engineering and its preparation method

A technology of genetic engineering and chimeric peptides, applied in the field of genetic engineering, can solve problems such as increasing the production cost of compound vaccines and complicating vaccine preparation procedures

Inactive Publication Date: 2003-10-08
FUDAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are obviously many obstacles to the implementation of this idea. For example, the separate synthesis of the two synthetic peptides, and then the steps of coupling with TT or DT protein carriers that provide Th-cell epitopes, etc., all make the vaccine preparation process difficult. Complicated, it will also greatly increase the production cost of this compound vaccine

Method used

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  • Synthetic chimeric peptide of human chorionic gonadotrophin genetic engineering and its preparation method
  • Synthetic chimeric peptide of human chorionic gonadotrophin genetic engineering and its preparation method

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Embodiment Construction

[0029] Example of hCG Chimeric Peptide CP7

[0030] Materials and methods

[0031] 1. Restriction enzymes EcoR I, Sal I, Nde I, and BamH I are products of Boehringer Mannheim in the United States, T4 DNA ligase is a product of British Biolabs, T4 polynucleotide kinase is a product of New England Biolabs in the United States, and Taq I DNA polymerase It is a product of the State Key Laboratory of Genetic Engineering of Fudan University, IPTG is a product of Promega in the United States, protein low molecular weight standards and alkaline phosphatase-linked goat anti-mouse secondary antibody (IgG / HRP) are products of Huamei Bioengineering Company, 5-bromo-4- Chloro-3-indole-β-D-galactoside (X-gal) and isopropylthio-β-D-galactoside (IPTG) are products of Sigma, USA, which recognize hCGβ 133-139 The sequenced monoclonal antibody OT3A is a product of OrganonTechnika in the Netherlands.

[0032] 2. Cloning and sequencing The vector pBluescript KS (PBS) was purchased from Stratagen...

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Abstract

A kind of human chorionic gonadotropic homone (hCG) chimeric peptides CP7 (and its derivative) and CP11 is disclosed, whose coding gene can be expressed in colibacillus. Its expression resultant can be obtained by preparing SDS-polyacrylamide gel electrophoresis (SDS-PAGE). Then can be used to prepare hCG peptide vaccine source for the purpose of treating tumor and contraception.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and specifically relates to a hCG biosynthetic chimeric peptide (CP7 and CP11) and a preparation method thereof. Background technique [0002] Human chorionic gonadotropin (hCG) is a placental glycoprotein hormone that maintains human early pregnancy and is also expressed in common malignant tumors (Triozzi P and Stevens VC. Humanchorionic gonadotropin as a target for cancer vaccines. Oncol Rep 6:7-17, 1999), whose heterodimer is composed of α and β chains. As a contraceptive and / or tumor therapeutic vaccinogen, the natural hCGβ chain and its very specific carboxy-terminal (CTP) chemically synthesized 37 peptide (hCGβ-CTP) has been used. The former hCGβ heterodimer (HSD-hCG) vaccine coupled with the sheep LHα subunit has passed the human clinical phase II efficacy test (Talwar GP, et al.Avaccine that prevents pregnancy in women.Proc Natl Acad Sci USA 91: 8523-8536), but due to the h...

Claims

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Application Information

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IPC IPC(8): C07K14/59C12N15/16C12N15/70
Inventor 谢毅徐万祥应康邱德义顾少华廖矛川
Owner FUDAN UNIV
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