Preparation method of early pregnancy test paper

A technology for detecting test strips and detection lines, which is applied in biological testing, measuring devices, material inspection products, etc., and can solve problems such as false positives and mistesting of drugs or diseases

Pending Publication Date: 2021-09-10
HUBEI MEIBAO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, most of the early pregnancy test strips on the market use the colloidal gold method for detection. However, this method may cause false positive results. For example, some drugs or diseases may cause false positive results.

Method used

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  • Preparation method of early pregnancy test paper

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Experimental program
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Effect test

Embodiment 1

[0020] Embodiment 1: the preparation method of a kind of early pregnancy detection test paper disclosed by the present invention, comprises the following steps,

[0021] S1 Prepare the sample pad

[0022] a. Select glass fiber products made of low-density fibers with an average length of 50 μm and an average diameter of 0.9 μm, and cut the glass fiber mat into glass fiber mats of 2 cm*30 cm;

[0023] b. Use 13.00g borax buffer, 5.02gPPG-3 octyl ether, 1.00mol sodium pyrrolidone carboxylate, 1.00mol ethylenediaminetetraacetic acid, 0.25mol bovine serum albumin, 0.62mol heterophilic antibody, 0.23mol casein , 2.00g of chlorphenesin and an appropriate amount of water to prepare 1L of sample loading solution;

[0024] c. After washing the glass fiber mat in step a in 0.01mol / L phosphate washing solution, soak it in the sample loading treatment solution in step b for 20min, take it out and dry it for later use, and obtain the sample loading pad.

[0025] S2 Preparation of absorbe...

Embodiment 2

[0038] Embodiment 2: the preparation method of a kind of early pregnancy detection test paper disclosed by the present invention, comprises the following steps,

[0039] S1 Prepare the sample pad

[0040] a. Select glass fiber products made of low-density fibers with an average length of 100 μm and an average diameter of 0.8 μm, and cut the glass fiber mat into glass fiber mats of 2 cm*30 cm;

[0041] b. Use 13.00g borax buffer, 5.02gPPG-3 octyl ether, 1.00mol sodium pyrrolidone carboxylate, 1.00mol ethylenediaminetetraacetic acid, 0.25mol bovine serum albumin, 0.62mol heterophilic antibody, 0.23mol casein , 2.00g of chlorphenesin and an appropriate amount of water to prepare 1L of sample loading solution;

[0042] c. After washing the glass fiber mat in step a in 0.01mol / L phosphate washing solution, soak it in the sample loading treatment solution in step b for 30min, take it out and dry it for later use to obtain a sample loading pad.

[0043] S2 Preparation of absorbent ...

Embodiment 3

[0056] Embodiment 3: a method for preparing an early pregnancy detection test paper disclosed by the present invention, comprising the following steps,

[0057] S1 Prepare the sample pad

[0058] a. Select glass fiber products made of low-density fibers with an average length of 300 μm and an average diameter of 0.5 μm, and cut the glass fiber mat into 2cm*30cm glass fiber mats;

[0059] b. Use 13.00g borax buffer, 5.02gPPG-3 octyl ether, 1.00mol sodium pyrrolidone carboxylate, 1.00mol ethylenediaminetetraacetic acid, 0.25mol bovine serum albumin, 0.62mol heterophilic antibody, 0.23mol casein , 2.00g of chlorphenesin and an appropriate amount of water to prepare 1L of sample loading solution;

[0060] c. After washing the glass fiber mat in step a in 0.01mol / L phosphate washing solution, soak it in the sample loading treatment solution in step b for 24min, take it out and dry it for later use to obtain a sample loading pad.

[0061] S2 Preparation of absorbent pad

[0062] ...

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Abstract

The invention relates to the technical field of medical test reagents, and discloses a preparation method of early pregnancy test paper, wherein the preparation method comprises the following steps: preparation of a sample loading pad, preparation of a water absorption pad, preparation of a detection pad, and assembly and cutting of the test paper to obtain the early pregnancy test paper. The preparation method has the following advantages and effects that a human chorionic gonadotropin beta core fragment and human chorionic gonadotropin regular molecules in urine are qualitatively detected by optimizing the preparation processes of the sample loading pad, the water absorption pad and the detection pad and applying the principles of a double-antibody sandwich method and an immunochromatography method, so that while the detection efficiency is considered, the method has the advantages of high detection sensitivity and accuracy.

Description

technical field [0001] The invention relates to the technical field of medical testing reagents, in particular to a method for preparing early pregnancy test paper. Background technique [0002] Early pregnancy test strips usually use the principle of immunochromatography double-antibody sandwich method to make HCG test strips, which are reliable indicators to assist clinical judgment of pregnancy. At present, when the early pregnancy test paper is tested, when the tested urine sample siphons through the colloidal gold-labeled antibody, an antigen-antibody colloidal gold complex is formed, and the complex continues to crawl, and when it passes through the coated anti-HCGα subunit Mcab, a double antibody is formed The sandwich colloidal gold complex shows a color band at the coating line, and the excessive colloidal gold antibody continues to crawl, and forms a colloidal gold immune complex with the goat anti-mouse control line, showing a color band to confirm whether a woman...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/76G01N33/558G01N33/58G01N33/531
CPCG01N33/76G01N33/558G01N33/587G01N33/531G01N2800/36
Inventor 李恩良
Owner HUBEI MEIBAO BIOTECH
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