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hCG cycle detection test paper, hCG cycle detection kit, and preparation method and applications of hCG cycle detection test paper

A technology of periodic detection and test strips, which is applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of complex operation methods and detection methods, and achieve the effect of easy operation, accurate detection and simple method.

Inactive Publication Date: 2018-11-06
NANTONG EGENS BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Therefore, in view of the defects that the colloidal gold method detection agent in the prior art needs 3 detection test papers and pre-treatment of the urine to detect the number of days of pregnancy, resulting in complicated operation methods and detection methods, the present invention provides a detection method with few test papers and no Urine treatment is required, the operation and detection are simple, and the hCG cycle detection test paper, kit, and preparation method and application thereof suitable for home detection of pregnancy days

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  • hCG cycle detection test paper, hCG cycle detection kit, and preparation method and applications of hCG cycle detection test paper
  • hCG cycle detection test paper, hCG cycle detection kit, and preparation method and applications of hCG cycle detection test paper
  • hCG cycle detection test paper, hCG cycle detection kit, and preparation method and applications of hCG cycle detection test paper

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Embodiment 1

[0052] This embodiment provides a test paper for hCG periodic detection, including a first test paper strip 1 and a second test paper strip 2, and the first test paper strip 1 and the second test paper strip 2 respectively include: a base sheet and a The sample loading pad, colloidal gold adsorption pad, antibody-carrying membrane and water-absorbing pad adhered in sequence on the substrate, and the sample loading pad, colloidal gold adsorption pad, antibody-carrying membrane and water-absorbing pad are sequentially and only adjacent to each other. The parts are in contact with each other and partly overlapped; the antibody-carrying film is adhered to the middle part of the substrate, and there are spaced detection lines and quality control lines arranged thereon, and the detection lines are close to the colloidal gold adsorption pad, so The quality control line is close to the water-absorbing pad; wherein, the colloidal gold-anti-human chorionic gonadotropin β-hCG monoclonal a...

Embodiment 2

[0059] This embodiment provides a method for preparing the hCG cycle detection test paper of Embodiment 1, comprising the following steps:

[0060] 1. Preparation of nitrocellulose membrane:

[0061] (1) Select a nitrocellulose membrane with a pore size of 3um to 10um, and cut the membrane into specifications with a width greater than or equal to 2.0cm and a length of 30.5cm according to needs, and set aside;

[0062] (2) Use 0.1M phosphate buffer to prepare anti-α-HCG monoclonal antibody 1.3 mg / ml for the test line coating and use 0.85% sodium chloride buffer to prepare the quality control line for coating Anti-mouse IgG polyclonal antibody 2.0mg / ml;

[0063] (3) Select the antibody-coated surface of the nitrocellulose membrane and mark it, and coat the antibody solution of the detection line and the antibody solution of the quality control line to be coated on the membrane in parallel and evenly, and the detection line and the quality control line The distance between the ...

Embodiment 3

[0082] This embodiment provides a method for preparing the hCG cycle detection test paper of Embodiment 1, comprising the following steps:

[0083] 1. Preparation of nitrocellulose membrane:

[0084] (1) Select a nitrocellulose membrane with a pore size of 3um to 10um, and cut the membrane into specifications with a width greater than or equal to 2.0cm and a length of 30.5cm according to needs, and set aside;

[0085] (2) Use 0.1M phosphate buffer to prepare 1.1 mg / ml of anti-α-HCG monoclonal antibody for testing line coating and use 0.85% sodium chloride buffer to prepare quality control line for coating Anti-mouse IgG polyclonal antibody 2.5mg / ml;

[0086] (3) Select the antibody-coated surface of the nitrocellulose membrane and mark it, and coat the antibody solution of the detection line and the antibody solution of the quality control line to be coated on the membrane in parallel and evenly, and the detection line and the quality control line The distance between the li...

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Abstract

The invention discloses hCG cycle detection test paper, which comprises a first test paper strip and a second test paper strip, wherein the first test paper strip and the second test paper strip respectively comprise a substrate, a sample loading pad, a colloidal gold adsorption pad, an antibody carrying film and a water absorption pad, the sample loading pad, the colloidal gold adsorption pad, the antibody carrying film and the water absorption pad are sequentially adhered to the substrate, the antibody carrying film is provided with a detection line and a quality control line at intervals, the colloidal gold adsorption pad on the first test paper strip adsorbs a colloidal gold-anti-human chorionic gonadotropin beta-hCG monoclonal antibody conjugate, the colloidal gold adsorption pad on the second test paper strip adsorbs a colloidal gold-anti-human chorionic gonadotropin beta-hCG monoclonal antibody conjugate and free beta antibody, the detection line is coated with alpha-hCG monoclonal antibody, and the quality control line is coated with an anti-mouse IgG polyclonal antibody. According to the present invention, the detection test paper does not need to treat the urine sample, has characteristics of simple operation and simple detection, is suitable for on-site, instant and rapid detection, and is suitable for the detection of gestational weeks in the family.

Description

technical field [0001] The invention belongs to the field of biological detection, and in particular relates to a hCG cycle detection test paper, a kit, a preparation method and an application thereof. Background technique [0002] Human chorionic gonadotropin (hCG) is a glycoprotein hormone secreted by placental trophoblast cells during pregnancy. It consists of two different subunits, α and β, which are connected by non-covalent bonds. In the process of hormone production, secretion, metabolism, etc., hCG molecules will find various changes such as fragmentation and dissociation, and thus exist in various molecular forms in blood and urine. hCG is the only placental hormone that does not increase with the increase of placental weight. The reference value of hCG for pregnant women is recorded in the "National Clinical Laboratory Operation Regulations". The reference value of hCG is 5-50 IU / ml for 0.2-1 week of pregnancy; 2 weeks, hCG reference value is 50-500IU / ml; 2-3 wee...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/76G01N33/558
CPCG01N33/76G01N33/532G01N33/558G01N33/54386G01N2333/59G01N33/54387
Inventor 欧卫军孙一品徐艳
Owner NANTONG EGENS BIOTECH CO LTD
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