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Novel method for determining human chorionic gonadotropin

A chorionic gonadotropin and new method technology, applied in biological testing, measuring devices, material inspection products, etc., can solve the problem of insufficient stability of immune sensors and achieve excellent stability and high sensitivity

Active Publication Date: 2020-01-17
GUANGXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional electron mediators such as thionine, methylene blue, alizarin red and ferrocene cannot be stably fixed on the electrode, so the constructed immunosensor is not stable enough

Method used

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  • Novel method for determining human chorionic gonadotropin
  • Novel method for determining human chorionic gonadotropin
  • Novel method for determining human chorionic gonadotropin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] 1 Experimental method

[0048] 1.1 Reagents and instruments

[0049] HCG antigen and HCG antibody (anti-HCG), antigen CEA, antigen AFP, and antigen CA15-3 were purchased from Biocell Co. (Zhengzhou, China); graphene oxide (GO) (Nanjing Xianfeng Nano Technology Material Technology Co., Ltd., Nanjing, China) ). Silver nitrate, sodium citrate, disodium hydrogen phosphate, potassium dihydrogen phosphate, and potassium persulfate (Guangdong Shantou Xilong Chemical Co., Ltd., China); bovine serum albumin (BSA), N-hydroxysuccinimide (NHS ) and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), polyethyleneimine (PEI), 1,1'-ferrocenedicarboxylic acid (Fc-COOH ), methanol and sodium hydroxide (available from Aladdin Industries). All the reagents used in the experiment were of analytical grade, and the water was ultrapure water.

[0050] ECL responses were recorded using an MPI-B ECL analyzer (Xi'an Remex Electronic Technology Co., Ltd., Xi'an, China); the voltage of the p...

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Abstract

The invention discloses a novel method for determining human chorionic gonadotropin. The method comprises the following steps of: by taking waste biomass papaya peel as a raw material, synthesizing blue high-fluorescence carbon quantum dots in one step by adopting a hydrothermal method, and combining the blue high-fluorescence carbon quantum dots with silver ions to form AgCQDs; and synthesizing PS through photolysis and coordination polymerization of ferrocene dicarboxylic acid molecules in methyl alcohol, and combining AgCQDs with negative electricity and PS modified by PEI to be changed into PS with positive electricity through electrostatic attraction, wherein the obtained composite material and an rGO@Ag composite material jointly prepare a sandwich type immunosensor for HCG detection. The method is high in sensitivity and good in stability.

Description

technical field [0001] The invention relates to a detection method for human chorionic gonadotropin, in particular to a new method for measuring human chorionic gonadotropin. Background technique [0002] Human chorionic gonadotropin (HCG) is a 37kDa glycoprotein hormone produced by trophoblast cells of the placenta during pregnancy and overexpressed by trophoblastic tumors and various non-fibroblastic tumors. Furthermore, HCG, as a biomarker, plays an autocrine role in human cancer biology, promoting tumor growth, invasion, and malignancy. Sensitive and accurate detection of HCG in serum is of great significance for predicting pregnancy and can be used as a tumor marker for monitoring trophoblastic diseases. In reported work, various techniques have been used to detect HCG, including electrochemical immunoassay (EC), fluorescence method (FL), surface-enhanced Raman scattering spectroscopy (ERS), chromatographic test strips, chemiluminescence immunoassay ( CE) and photolum...

Claims

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Application Information

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IPC IPC(8): G01N27/30G01N27/327G01N21/76G01N33/76
CPCG01N21/76G01N27/308G01N27/3277G01N27/3278G01N33/76
Inventor 邓必阳覃东庙蒋小华莫桂春
Owner GUANGXI NORMAL UNIV
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