Using and preparation methods of human chorionic gonadotropin test strip for full-automatic analyzer

Abstract

The invention discloses a using and preparation methods of a human chorionic gonadotropin test strip for a full-automatic analyzer. The preparation method comprises the following steps: adhering and fixing a coating film to a PVC rubber plate; sequentially assembling a sample pad, a colloidal gold combination pad and a water absorption material which are configured according to the requirements onto the PVC rubber plate with the coating film; adhering a protective film adhesive tape containing a warning line on the sample pad according to the requirements; adhering a protective film adhesive tape containing a warning line to the water absorption paper strip; carrying out cutting to obtain a test strip; testing the lowest detection limit of the test strip; and carrying out positive / negativespecificity analysis with high-concentration hLH, hFSH, hLH and hTSH samples. According to the using method, about 60[mu]L of a sample urine, serum or plasma sample is added to a sample pad area of the test strip by using a full-automatic analyzer sample adding needle or manually using a dropper or a dropper / pipette. Positions of the detection line and the quality control line are relatively fixed, so that full-automatic instrument result interpretation is facilitated, the clinical application value is achieved, and the inspection efficiency can be greatly improved.

Description

technical field [0001] The invention belongs to the technical field of medical detection, and in particular relates to a method for using and preparing a human chorionic gonadotropin test strip that can be used in an automatic analyzer. Background technique [0002] HCG is human chorionic gonadotropin, which is the acronym of human chorionic gonadotrophin in English. It is a glycoprotein secreted by trophoblast cells of the placenta. It is composed of glycoproteins of α and β dimers , HCG detection test strips can accurately detect the concentration of HCG in human urine, so that women can know the information of pregnancy at the earliest; currently clinical urine test hCG commonly used gold-labeled antibody assay method to detect human chorionic gonadotropin, the detection cost is low, the speed is fast, and it is suitable Bedside or point-of-care testing. [0003] However, there are still some unreasonable factors in the use and preparation of the existing human chorionic...

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Problems solved by technology

[0004] The purpose of the present invention is to provide a kind of use and the preparation method of the human chorionic gonadotropin test paper strip that can be used for automatic analyzer, to solve the existing human chorionic gonadotropin test paper strip proposed in the above-mentioned background technology There are still some unreasonable factors in the use and preparation process of the existing human chorionic gonadotropin test strips. When using the gold-labeled antibody method test strips in the use and preparation process of the existing human chorionic gonadotropin test strips, it is inconvenient to detect because of its small size. ;The front-end sample pad is attached with a protective film tape to absorb water slowly, and the inspector needs to hang the hand above the fixed-point sample. When the sample size is large, it is easy to make the inspector fatigue and reduce the work efficiency.

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