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Systhesizing of chimeric peptide from anteron promoted by human's fine hair

A chimeric peptide and multi-epitope technology, applied in the field of genetic engineering, can solve the problems of complicated vaccine preparation procedures and increased production costs of compound vaccines

Inactive Publication Date: 2003-08-27
SHANGHAI INST OF PLANNED PARENTHOOD RES +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are obviously many obstacles to the implementation of this idea. For example, the separate synthesis of the two synthetic peptides, and then the steps of coupling with TT or DT protein carriers that provide Th-cell epitopes, etc., all make the vaccine preparation process difficult. Complicated, it will also greatly increase the production cost of this compound vaccine

Method used

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  • Systhesizing of chimeric peptide from anteron promoted by human's fine hair
  • Systhesizing of chimeric peptide from anteron promoted by human's fine hair
  • Systhesizing of chimeric peptide from anteron promoted by human's fine hair

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0029] Example of hCG Chimeric Peptide CP1

[0030] Materials and methods

[0031] 1. Restriction enzymes EcoR I, Sal I, Nde I, and BamH I are products of Boehringer Mannheim in the United States, T4 DNA ligase is a product of British Biolabs, T4 polynucleotide kinase is a product of New England Biolabs in the United States, and Taq I DNA polymerase It is a product of the State Key Laboratory of Genetic Engineering of Fudan University, IPTG is a product of Promega in the United States, protein low molecular weight standards and alkaline phosphatase-linked goat anti-mouse secondary antibody (IgG / HRP) are products of Huamei Bioengineering Company, 5-bromo-4- Chloro-3 indole-β-D-galactoside (X-gal) and isopropylthio-β-D-galactoside (IPTG) are products of Sigma, USA, which recognize hCGβ 133-139 The sequenced monoclonal antibody OT3A is a product of OrganonTechnika in the Netherlands.

[0032] 2. Cloning and sequencing The vector pBluescript KS (PBS) was purchased from Stratagen...

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Abstract

The invention refers to human chorion gonadotropic hormone (hCG) chimera peptide CP1, ramification CP10, and the making method by gene engineering. They have 6 amino (N) ends or 5 continuous broad-spectrum or single strong T-cell table, and 3 B-cell tables of hCG beta-antigen at carboxylic end: beta5 45-52, beta9 113-116 and beta8 137-144. The genes coding CP1 and CP10 can all be expressed in colibacillus, and the expressed product can be obtained by the SDS-polyacrylamide gelatin electrophoresis (SDS-PAGE).

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and specifically relates to a biosynthetic chimeric peptide (CP1 and CP10) of human chorionic gonadotropin and a preparation method thereof. Background technique [0002] Human chorionic gonadotropin (hCG) is a glycoprotein hormone that maintains early pregnancy in humans, and it consists of two subunits, α and β. As a contraceptive and / or tumor therapeutic vaccinogen, the natural hCGβ subunit or its very specific carboxy-terminal chemically synthesized 37 peptide (hCGβ-CTP) has been used. The former hCGβ heterodimer (HSD-hCG) vaccine coupled with sheep LHα subunit has passed the human clinical phase II efficacy test (Talwar GP, et al.A vaccine that prevents pregnancy in women.Proc Natl Acad Sci USA 91 : 8523-8536, 1994), but due to the high cost of hCGβ separation and purification, the need for macromolecular protein carriers such as tetanus toxoid (TT) and / or diphtheria toxoid (DT)...

Claims

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Application Information

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IPC IPC(8): C07K14/59C12N15/16C12N15/70
Inventor 徐万祥谢毅邱德义应康廖矛川顾少华
Owner SHANGHAI INST OF PLANNED PARENTHOOD RES
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