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Beta-human chorionic gonadotrophin near-infrared fluorescence detection reagent card, kit and purpose thereof

A chorionic gonadotropin and detection kit technology, applied in fluorescence/phosphorescence, measurement device, material excitation analysis, etc., can solve the problems of disease diagnosis obstacles, low accuracy of quantitative detection results, etc., to improve accuracy and precision , reduce the error, avoid the effect of mutual interference

Inactive Publication Date: 2018-04-20
SHANGHAI CHEMTRON BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Judging by the color of a test line is easily affected by the brightness and color of the environment, and is also affected by the subjective judgment of the user, resulting in a large error, which makes the detection results, especially the quantitative detection results, less accurate, which is the cause of disease. Diagnosis brings obstacles

Method used

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  • Beta-human chorionic gonadotrophin near-infrared fluorescence detection reagent card, kit and purpose thereof

Examples

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Effect test

preparation example Construction

[0048] The present invention also provides a preparation method of the β-human chorionic gonadotropin near-infrared fluorescence detection reagent card described above, comprising the following steps: 1) spraying a fluorescent probe-first antibody on the glass cellulose membrane A mixed solution of β-human chorionic gonadotropin antibody and fluorescent probe test antigen; 2) use the first pretreatment solution to carry out the first pretreatment on the nitrocellulose membrane corresponding to the T1 line to be sprayed, and in the first Spray the second anti-β-human chorionic gonadotropin antibody solution on the pretreated nitrocellulose membrane; 3) use the second pretreatment solution to perform the second pretreatment on the nitrocellulose membrane corresponding to the T2 line to be sprayed , and spray the β-human chorionic gonadotropin antigen solution on the nitrocellulose membrane after the second pretreatment; 4) spray the antibody solution of the test antigen on the ni...

Embodiment 1

[0057] 11. Preparation of near-infrared fluorescence detection reagent card for β-human chorionic gonadotropin, the specific method includes the following steps:

[0058] 111. Preparation of the mixed solution of fluorescent probe-first anti-β-human chorionic gonadotropin antibody and fluorescent probe-test antigen:

[0059] Add the required amount of mouse anti-β human chorionic gonadotropin monoclonal antibody (product number RS-003-0102) and green fluorescent protein from Shanghai Rongsheng Biotechnology Co., Ltd. to the preset volume of 0.05M PBS, and then add The mouse anti-β human chorionic gonadotropin monoclonal antibody (Cat. No. RS-003-0102) of Shanghai Rongsheng Biotechnology Co., Ltd. was conjugated to green fluorescent protein by EDC; EDC was removed through centrifugation, dilution and other operations, and the obtained fluorescence Probe - Primary anti-β-hCG antibody solution. Add the required amount of rabbit IgG and green fluorescent protein to the preset vol...

Embodiment 2

[0081] 21. Preparation of β-human chorionic gonadotropin near-infrared fluorescence detection reagent card

[0082] The specific preparation method refers to the description of steps 111-1111 in Example 1, wherein, the difference is that the first pretreatment liquid formula in this embodiment is: arginine 1.75g / L, potassium hydrogen phthalate 1.5g / L, sodium hydroxide 0.3g / L, citric acid 2.5g / L, solvent is water, pH value=6.0. The formula of the second pretreatment liquid is: asparagine 2.0g / L, disodium hydrogen phosphate 0.7g / L, rhamnose 3.5g / L, glycine 3.0g / L, solvent is water, pH=8.0.

[0083] 22. Use the β-human chorionic gonadotropin near-infrared fluorescence detection reagent card provided in this example. β-human chorionic gonadotropin antigen was detected. For the detection process, refer to step 121 and step 122 in Embodiment 1. The test results were 0.0204mIU / ml, 2.001mIU / ml, 20.005mIU / ml, 200.003mIU / ml, 2000.002mIU / ml, 10000.006mIU / ml respectively, and no β-huma...

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Abstract

The invention relates to the field of field of fluorescence immunochromatography, and concretely relates to a beta-human chorionic gonadotrophin near-infrared fluorescence detection reagent card, a kit and a purpose thereof. The beta-human chorionic gonadotrophin near-infrared fluorescence detection reagent card comprises a sampling gasket, a glass cellulose membrane, a cellulose nitrate membrane,and a water-absorption gasket arranged in order; wherein, the glass cellulose membrane contains a fluorescence probe-first anti-beta human chorionic gonadotrophin antibody and a fluorescence probe-test antigen; and a first test line, a second test line and a control line are arranged on the cellulose nitrate membrane in order; the first test line is coated with a second anti-beta human chorionicgonadotrophin antibody; the second test line is coated with the beta-human chorionic gonadotrophin antigen; and the control line is coated with the antibody of the test antigen.

Description

technical field [0001] The invention relates to the field of fluorescence immunochromatography, in particular to a beta human chorionic gonadotropin near-infrared fluorescence detection reagent card, kit and application thereof. Background technique [0002] β-human chorionic gonadotropin (β-HCG) is a glycoprotein secreted by trophoblast cells of the placenta, which is the β subunit of human chorionic gonadotropin. The content of normal human β-HCG is about 0-3mIU / ml. The blood β-HCG ratio can be used as a judgment indicator for intrauterine pregnancy, ectopic pregnancy and pregnancy failure. Patients with a β-HCG ratio <0.81 are more likely to have a pregnancy failure, and those with a ratio greater than 1.68 are more likely to have an intrauterine pregnancy. [0003] Therefore, the detection of β-human chorionic gonadotropin (β-HCG) is of great significance. [0004] The immunodiagnostic reagents in the prior art, whether they are qualitative or quantitative reagents,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/359G01N21/64G01N33/533
CPCG01N21/359G01N21/6428G01N33/533
Inventor 丁晓辉
Owner SHANGHAI CHEMTRON BIOTECH
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