34 results about "Hormone injections" patented technology

The invention relates to a totally artificial breeding method for Coilia ectenes, belonging to the technical field of fish culture. The method is characterized by comprising the following process steps of parent fish source; parent fish select breeding comprising standing / flowing water combined reinforced breeding; artificial induced spawning measure comprising parent fish selection, semen collection, dug roe inspection, induced spawning water temperature confirmation, parent fish matching, hormone injection, artificial insemination and the like; microflow water incubating; and summer flower and fish fry breeding. The invention promotes the parent fish sex gland to grow to maturity and ovulate by measures of reinforcing parent fish nutrition reinforced breeding, feeding hormone, flowing water stimulating and regulating and the like and normalizes the artificial induced spawning and insemination process, originally creates a simple flowing water incubating device of a pelagic egg and reinforces the management and ecological regulation of incubation period, thereby effectively solving the technical problems of artificially breeding Coilia ectenes. The induced spawning rate of artificial breeding is 33 percent, the fertilization rate is 48 percent and the hatching rate is 44 percent.

The invention relates to an artificial eel propagation method, which includes: a, ripening parent fishes, breeding catadromous parent reels for seven days, gradually increasing salinity of water, and subjecting the parent eels to back intramuscular injection of ripening agent-carp pituitary gland homogenate and HCG (human chorionic gonadotropin); b, performing induced spawning of the parent eels, subjecting female eels to injection of oxytocin 17 alpha-hydroxyl progesterone and HCG, subjecting sperm-producing male eels to injection of mixture of CPE (carp pituitary extract) with HCG, collecting naturally spawned fertilized eggs on the next day, and subjecting the female eels laying no eggs to semi-dry artificial insemination; and c, hatching the fertilized eggs, immediately transferring the fertilized eggs to a special-made hatching barrel for hatching, and slightly aerating, changing half of water every four hours until fry are hatched successfully. The artificial eel propagation method has the advantages that anesthetic loss and operational injury can be prevented, injection dosage of hormones is reduced, a great amount of eels can mate and lay eggs naturally under artificial induced spawning, artificial insemination can be complete securely in a planned way, level of high-quality fertilized eggs and alevin of eels artificially propagated is increased.

The invention discloses an efficient spawning induction method of misgurnus anguillicaudatus. The method comprises steps as follows: (1) selection of parents; (2) pretreatment of the parents; (3) agent injection; (4) collection of zygotes. Under the combined action of pretreatment of misgurnus anguillicaudatus and treatment with a spawning promotion stabilizer during mixed culture after hormone injection, the harm caused by human factors to the misgurnus anguillicaudatus is reduced, the anti-stress capacity of the misgurnus anguillicaudatus is improved, the yield, the quality and the breeding speed of zygotes are increased, and the method causes no harm to the misgurnus anguillicaudatus, human bodies and the environment, is environment-friendly and safe and has high popularization value.

The invention discloses a full artificial breeding technology for lateolabrax japonicus in the northern area and belongs to the technical field of genetic breeding of aquatic products, providing a technical support for genetic breeding of lateolabrax japonicus. The full artificial breeding technology for lateolabrax japonicus in the northern area includes (1) the full artificial cultivation technology for lateolabrax japonicus in the northern area, which refers to techniques of cultivating parent fish in outdoor net cages in summer and autumn and cultivating parent fish in indoor cement pondsin spring and winter, (2) the artificial insemination and artificial incubation technology for lateolabrax japonicus in the northern area, which particularly includes techniques of selection of parentfish of lateolabrax japonicus, hormone injection, sperm and egg maturity determination, artificial insemination, one-to-one building of the full-sib family, and hatching of fertilized eggs. Accordingto the technology, female lateolabrax japonicus in four years or four years above, of which the weight is equal to or higher than 2.50kg, is in sexual maturity stage, and male lateolabrax japonicus in three years or three years above, of which the weight is equal to or higher than 2.00kg, is in sexual maturity stage.

The invention relates to a totally artificial breeding method for Coilia ectenes, belonging to the technical field of fish culture. The method is characterized by comprising the following process steps of parent fish source; parent fish select breeding comprising standing / flowing water combined reinforced breeding; artificial induced spawning measure comprising parent fish selection, semen collection, dug roe inspection, induced spawning water temperature confirmation, parent fish matching, hormone injection, artificial insemination and the like; microflow water incubating; and summer flower and fish fry breeding. The invention promotes the parent fish sex gland to grow to maturity and ovulate by measures of reinforcing parent fish nutrition reinforced breeding, feeding hormone, flowing water stimulating and regulating and the like and normalizes the artificial induced spawning and insemination process, originally creates a simple flowing water incubating device of a pelagic egg and reinforces the management and ecological regulation of incubation period, thereby effectively solving the technical problems of artificially breeding Coilia ectenes. The induced spawning rate of artificial breeding is 33 percent, the fertilization rate is 48 percent and the hatching rate is 44 percent.

The invention discloses a method for increasing the transcaucasian barb artificial reproduction fertility rate. The method comprises the following steps of 1, parent fish preparation; 2, artificial hormone injection, wherein a two-time injection method is adopted for performing artificial hormone injection, and the time interval between two times of needle injection is 24 h; according to the female transcaucasian barb injection dosage, the first-time injection dosage is 4 micrograms per kilogram of parent transcaucasian barb, and the second-time injection dosage is 4 micrograms of LRH-A, 800 IU of HCG, 3-5 of PG and 2,800 IG of LRH-A per kilogram of parent transcaucasian barb, and the male transcaucasian barb injection dosage is 1 / 2 of the female transcaucasian barb injection dosage; 3, artificial insemination; 4, micro running water culture fertility rate. The two-time injection method is adopted for artificial injection, and the time interval is 24 h; a semidry method is adopted for artificial insemination, a certain amount of ringer solution is added, and the transcaucasian barb artificial reproduction fertility rate can be remarkably increased, so that the key technical problem of transcaucasian barb artificial reproduction is solved, and then guarantees are provided for fry culture and commercial fish artificial reproduction.

The present invention introduces the preparation process of injection microballoon with PLGA, PLA or other biodegradable polymer as skeleton material and the progestational hormone, gestrinone, as model medicine, and through solvent evaporation. The microballoon may be injected to subcutaneous part or muscle for delayed releasing for over 15 days. The microballoon skeleton material inside body degrades gradually into small molecular matter excreted to outside the body. The gestrinone microballoon injection may be administrated conveniently and is superior to conventional tablet form, which is orally taken frequently to result in damage to liver and kidney and other negative reaction. Rat experiment shows that the preparation of the present invention may be used in treating endometriosis, etc.

The invention discloses a bottom water filtering system and further discloses an imitated ecological recirculating aquaculture and propagation system and a method for promoting natural propagation of migratory fish by adopting the recirculating aquaculture and propagation system. By adopting the method and establishing the recirculating aquaculture and propagation system suitable for integrated growth, development and spawning of the migratory fish, breeding and propagation functions are effectively integrated, and synergic regulation of ecological factors which are necessary during maturation of the migratory fish, especially reeves shad is optimized, so that sexual glands of bred parent fish can synchronously develop and mature under the appropriate, coordinative and controllable ecological conditions, the parent fish can naturally spawn without artificial spawning, and injuries, deaths and gonad development interference caused by hormone injection and catching operation are avoided. The method for promoting the natural propagation of the migratory fish by adopting the recirculating aquaculture and propagation system is simple, reliable and effective and is the key for propagation technology industrialization of the migratory fish, especially the America reeves shad.

The invention discloses a saprolegniasis prevention and control method for gymnocypris potanini. The method includes the steps that A, a flowing water breeding barrel is needed, flowing water breeding is performed, and ammonia nitrogen is controlled below 0.05 mg / L; B, water flow is controlled, wherein the speed of flowing water is controlled through a water pump or by adjusting the direction and the magnitude of water flow of a water inlet; C, throwing for feeding is performed, wherein after temporary raising and food domesticating, rainbow trout juvenile fish feed is thrown for feeding; D, drug use is performed, wherein the water amount is measured and calculated according to the area of a pond and the water depth, and oxytetracycline is splashed; E, vitamins are used, wherein vitamin C is splashed in the whole pond; and F, saprolegniasis prevention and control are performed for parent fish after delivery, wherein after hormone injection and spawning induction for egg squeezing, under the water temperature of a breeding season, and on the basis that daily prevention and control is kept, prevention and control measures include that a, soaking of glucose and a triphosadenine solution is performed after delivery; and b, antibiotics are thrown for feeding, wherein ampicillin is added in each kilogram of feed, and after stirring and uniform mixing, throwing for feeding is performed. The method is easy to execute, and easy and convenient to operate, effectively prevents generation of saprolegniasis in the breeding process of the gymnocypris potanini and after spawning induction, and a basis is laid for the species protection work of the gymnocypris potanini.

The present invention discloses a method for breeding and culturing hybridized snakehead fish, including steps of ripe fish selection, hormone injection, ripe fish pairing, fish-egg collection, fish-egg incubation and fish fry temporary culture. A snake-head is selected as a ripe fish male parent and a snakehead mullet is selected as a female parent, an precise hormone compatibility and an injection method are determined to inject hormone to the male parent and the female parent ripe fishes for an induced spawning, then breeding by single pairing one to one, collecting and incubating a fertilized ovum, then a fish fry may be transported and sell after culturing temporarily. By means of the method of the invention, a rate of hybridized snakehead fish emergence is high, a growth rate is rapid, a converse resistance is strong, and the fish fry is able to bear a transportation, the method of the invention has an important significance for a popularization and culture of the snakehead fishes.

The present invention provides a method for inducing twin calving. The method according to the present invention comprises firstly treatment to inducing atresia of the antral follicles on ovary so as to initiate the development of new follicular wave, inducing the development of codominant follicles and further achieving double ovulations by exactly controlling the dosage and time points of hormone injections, and performing artificial insemination to induce the cow to deliver twin calves. The method according to the present invention has relative independent on the technical skills, and a person skilled in the art or a common worker can practice it according to specified operation methods; and during practical application, it does not require special equipment, thus saving cost, as well as improving the social benefit and economic benefit of cow breeding.

The invention relates to a sow raising and breeding method. Through the method, a disperse, random and traditional raising method is converted into concentrated, continuous and balanced assembly line production according to ecological and physiological characteristics of sows, an all-in and all-out process is implemented, and annual balanced production is performed according to the breeding rhythm. The key technology of all-in and all-out production according to the breeding rhythm is estrus synchronization. Estrus synchronization of the sows can be achieved through three methods, namely (1) estrus synchronization of the multiparous sows is achieved through a simultaneous weaning method; (2) estrus synchronization of the sows experiencing the puberty or pregnant sows is achieved through a pen breeding and hormone injection method; (3) estrus synchronization of gilts which are not in the puberty is achieved through a group raising and hormone injection method. The sow raising and breeding method is experience obtained through many years of sow raising, enables the sows to bred regularly, facilitates production and operation management, and enables work to be conducted in an orderly and planned mode.

The invention discloses a method for imitating ecological artificial reproduction in an earthen pond of petaled fish, comprising the following steps: A, selecting a spawning pond; B, making and placing a fish nest: after covering the surface of a tile with dense gauze, the tile is symmetrically Fixedly set up a hollow elliptical column fish nest, put the fish nest in potassium permanganate solution for disinfection; wooden sticks are arranged in an array form in the spawning pool, and the distance between any wooden stick and the edge of the pool is ≥ 50cm, and each row of wooden sticks Two adjacent wooden sticks in the sticks are connected with a rope, and two adjacent wooden sticks in each row of wooden sticks are connected with a rope, and the sterilized fish nests are arranged on the rope at intervals and are 8-12cm away from the bottom of the pool; C, parent fish selection; D, Broodstock induction: When the water temperature exceeds 22°C for 7 consecutive days, inject hormones from the base of the pelvic fin: female fish are injected with pituitary hormone 0.013‑0.017 mg / kg, male fish are injected with dioconone and luteinizing hormone releasing hormone analogues, The injection dose is 0.004-0.006mg / kg; E, stocking of broodstock; F, daily management.