Artificial propagation method of clarias fuscus

Abstract

The invention discloses an artificial propagation method of clarias lazeli. The artificial propagation method comprises the following steps: S1, parent screening and breeding: selecting female clarias lazeli and male clarias anodontophaga as parent fishes; carrying out intensive cultivation on the parent fish; s2, parent fish screening and artificial spawning induction, wherein the selected parent fish is subjected to artificial spawning induction through hormone injection, and then artificial insemination is conducted; s3, hatching management of fertilized eggs: adopting a conical plastic barrel for hatching, and maintaining water in the conical plastic barrel to be in a flowing state through a circulating water system; s4, juvenile fish breeding, wherein after the juvenile fish is bred for 30 days, the juvenile fish with the dorsal fin height / body length larger than or equal to 15% is screened out for phenotypic optimization. According to the method, the appearance of the clarias lazeli and the growth speed of the clarias anodontoides are integrated through a hybridization technology, so that the progeny morphology of the clarias lazeli is close to that of the Giethiwei shark, and the industry is promoted to be upgraded towards the high value-added direction In the specific breeding method, the fertilization rate is effectively increased and the survival rate of juvenile fishes is increased through cooperation of multiple factors such as parent fish selection, breeding, water temperature of each stage, water flow, illumination and nutrition support.

Description

Technical Field

[0001] This invention relates to the field of aquaculture breeding technology, specifically to an artificial breeding method for giant catfish. Background Technology

[0002] giant catfish (scientific name: *Lagrange catfish*) Pangasius larnaudii The Largo dorsalis, also known as the Laurentie shark, is a large freshwater catfish found in the Mekong and Chao Phraya river basins of Southeast Asia. Wild individuals can reach a maximum length of 1.3 meters, but in captivity, they typically only grow to 40-60 centimeters. It is an important food fish in Southeast Asia, with firm flesh and no small bones, and is often processed into frozen fillets for export. Due to its unique appearance and active swimming habits, it is a common large ornamental fish in the aquarium market. The Largo dorsalis is primarily valued as an ornamental fish because of its shark-like appearance. Along with the Genghis Khan shark and the blue shark, it is considered a freshwater shark, with the Genghis Khan shark previously being the most sought-after, but now facing extinction due to overfishing. While the Largo dorsalis is similar in appearance to the Genghis Khan shark, it has a relatively shorter and stouter body, paler coloration, and slower growth rate and shorter body length in captivity.

[0003] To address the aforementioned shortcomings of the giant catfish (Mekong giant catfish), optimization can be achieved through interspecific hybridization. Hybridization of giant catfish has been studied in academic fields, such as in the report "The First Report on Chromosome Analysis of F1 Hybrid Catfish: Mekong Giant Catfish" by foreign scholar Kaewmad and Puntivar. Pangasianodon Gigas )×Striped Catfish ( Pangasianodon Hypophthalmus ) and Spot Pangasius Pangasius Larnaudii )×P angasianodon Hypophthalmus (Siluriformes, Pangasiidae). (CYTOLOGIA, International Society of Cytology, 2017) Regarding the comparison between *Siluriformes lasiocarpa* and *Siluriformes lasiocarpa* (… Pangasianodon hypophthalmus Artificial hybridization of giant catfish such as α-catfish has been explored and experimented with, but related papers have little information on specific breeding methods, especially those applied to large-scale aquaculture. Summary of the Invention

[0004] The purpose of this invention is to provide an artificial breeding method for giant catfish (Catella lataniae) to solve the problems existing in the prior art.

[0005] To achieve the above objectives, the present invention provides an artificial breeding method for *Catella lataniae*, comprising the following steps: S1, parent selection and cultivation: female *Catella lataniae* and male *Catella leucocephala* are selected as parent fish; the selected parent fish undergo intensive cultivation for 30-60 days before induced spawning; S2, broodstock selection and artificial spawning induction: female *Catella lataniae* at the end of gland maturity stage IV are selected; male *Catella leucocephala* with sperm motility ≥80% are selected as broodstock and artificially induced spawning is performed through hormone injection followed by artificial insemination; S3, fertilized egg hatching and management: conical plastic buckets are used for hatching, and the water in the conical plastic buckets is kept in a flowing state through a circulating water system; after hatching, the hatched fry are transferred to a nursery pond; S4, fry cultivation: after 30 days of fry cultivation, fry with a dorsal fin height / body length ≥15% are selected and phenotypically optimized by blue light irradiation.

[0006] Preferably, in step S1, the selection criteria for female *Catella raspyri* are 4-6 years old, weight greater than or equal to (3-5) kg, body length greater than 30 cm, and dorsal fin height / body length greater than 10%; the selection criteria for male *Catella simonii* are 5-6 years old, weight greater than or equal to 6 kg, body length greater than 70 cm, and dorsal fin length / body length greater than 10%. The intensive cultivation period in step S1 is 30-60 days before spawning induction, with a culture water temperature of 26±2℃, dissolved oxygen ≥5 mg / L, and pH 7.0-7.5; daily light exposure is no less than 14 hours, preferably using white or red LED light sources; pulsed water flow is provided at least twice daily; the feed formula for female *Catella raspyri* is 50% animal protein, 40% plant protein, 8% fat, and 2% fiber, with additives including astaxanthin, vitamin E, and vitamin C; the feed formula for male *Catella simonii* is 55% plant protein, 30% animal protein, 10% fat, and 5% fiber, with additives including β-glucan, bile acids, and vitamin B12. The amount of feed should be 2%-3% of body weight.

[0007] Preferably, in step S1, the pulsed water flow in the enhanced cultivation process is an intermittent variable frequency pulse, i.e., a flow rate of 0.5 m / s for 15-30 min, followed by an interval of 15-30 min and then a flow rate of 1 m / s for 15-30 min.

[0008] Preferably, the specific operation of step S2, the selection of breeding fish, is as follows: When selecting female Rasch's catfish, the female fish should have a swollen and soft abdomen, and the genital opening should be round, red, swollen, and everted; if the eggs are free and uniform in size when the middle and posterior part of the female fish's abdomen is gently pressed with a finger, it indicates that the maturity has reached the end of stage IV. Starting 15 days before spawning induction, the softness of the female fish's abdomen and the free state of the eggs should be checked every 3 days by palpation; 15 days before spawning induction, male fish semen should be sampled and its color should be observed visually. When it is milky white, it has reached maturity. When selecting male low-eyed toothless catfish, the male fish's abdomen should be gently pressed, and semen should be collected with a capillary tube and diluted in 0.7% physiological saline. Then, the semen sample should be observed under a 400x microscope to obtain the percentage of motile sperm.

[0009] Preferably, the specific operation of the artificial spawning in step S2 is as follows: For female Ragnar breeding catfish, a mixture of DOM and LRH-A2 is injected into the base of the pectoral fin twice, with an interval of 8-10 hours; for male low-eyed toothless catfish breeding fish, a mixture of LRH-A2 and 11-KT is injected into the base of the pectoral fin once.

[0010] Preferably, the specific operation of artificial insemination in step S2 is as follows: Egg collection: Two people cooperate to operate, wrap the female fish's body with sterile gauze, with the genital opening facing the sterile egg collection dish, and apply even pressure along the caudal peduncle to collect the eggs; Semen collection: Collect semen by live puncture, and dilute it with a mixed solution of NaCl 7.5g / L + KCl 0.2g / L + glucose 1g / L at a ratio of 1:5; Egg pretreatment: Wash the eggs of *Catella raspetus* with 0.1% trypsin combined with 0.05% hyaluronidase; Insemination: Mix the eggs and sperm at a ratio of 1:3, stir clockwise with poultry feathers for 30 seconds, let stand for 1 minute, rinse 3 times with 0.7% physiological saline to remove excess semen, and then soak in 0.3% talcum powder solution for 3-5 minutes for de-adhesion treatment, and rinse 3 times with clean water after de-adhesion treatment.

[0011] Preferably, step S3, fertilized egg incubation management, specifically includes the following process: an 80-mesh egg-attaching net is set at the bottom of the conical plastic incubator, and the water inside the conical plastic incubator is kept in a flowing state through a circulating water system. The water temperature is kept stable at 26-28℃, the dissolved oxygen content is ≥6mg / L, the pH value is 7.0-7.5, and the entire process is in a low-light environment. The light intensity is increased to 1000 lux 24 hours before hatching to stimulate hatching.

[0012] Preferably, the feeding of juvenile fish in step S4 is divided into three stages: 1-3 days old, rotifers are fed with Chlorella; 4-7 days old, Artemia nauplii, vitamin B12, and green algae are replaced; ≥8 days old, microparticle feed is fed with Bacillus subtilis added, the feeding amount is 15% of the fish's body weight, and it is fed 4 times a day.

[0013] Preferably, the specific operation for phenotypic optimization in step S4 of juvenile fish rearing is as follows: select fish with a dorsal fin height / body length ≥15% and expose them to 450nm blue light for at least 6 hours per day.

[0014] This invention integrates the physical characteristics of the giant catfish (Catella lataniae) with the growth rate of the toothless catfish (Catella simonii), using hybridization technology. Through selection, the offspring's morphology closely resembles that of the Genghis Khan shark (a critically endangered species). A single fish can fetch 3-5 times the price of ordinary edible fish, driving the industry towards higher added value. In the specific breeding method, the synergistic effect of multiple factors, including broodstock selection, cultivation, and incubation stages such as water temperature, water flow, light, and nutritional support, can effectively improve fertilization rates and juvenile survival rates. Detailed Implementation

[0015] The technical solutions of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0016] The artificial breeding method for *Catfish rasalis* involved in this invention includes the following steps: S1. Selection and breeding of parent fish: Select female Rasmus catfish aged 4-6 years with a weight greater than or equal to (3-5) kg, a body length greater than 30 cm, and a dorsal fin height / body length greater than 10%; and male Low-Eyed Toothless Catfish aged 5-6 years with a weight greater than or equal to 6 kg, a body length greater than 70 cm, and a dorsal fin length / body length greater than 10% as parent fish.

[0017] For the selected broodstock, intensive cultivation is carried out. The intensive cultivation period is 30-60 days before spawning induction. The water temperature is 26±2℃, dissolved oxygen is ≥5mg / L, and pH is 7.0-7.5. The daily light time is not less than 14 hours, and the light source is preferably white or red LED light source. There are at least 2 pulse water flows per day (flow rate 0.5-1m / s, lasting 30-60min). In order to further simulate the flood situation in nature, intermittent variable frequency pulses are used, that is, 0.5m / s flow rate for 15-30min, followed by 1m / s flow rate for 15-30min after an interval of 15-30min.

[0018] The feed formula for female *Catfish larvae* is 50% animal protein (40% fish meal + 10% krill meal), 40% plant protein (20% soybean meal + 20% wheat flour), 8% fat, and 2% fiber. Additives include astaxanthin 100mg / kg feed, vitamin E 50mg / kg feed, and vitamin C 1g / kg feed. The feed formula for male *Catfish larvae* is 55% plant protein (40% soybean meal + 15% corn gluten meal), 30% animal protein (25% fish meal + 5% blood meal), 10% fat, and 5% fiber. Additives include β-glucan 1g / kg feed, bile acids 0.5g / kg feed, and vitamin B12 0.1mg / kg feed. The feeding amount is 2%-3% of body weight.

[0019] During the intensive cultivation process, water quality should be monitored at least every two days, requiring that ammonia nitrogen in the aquaculture water be ≤0.2mg / L and nitrite be ≤0.1mg / L. If the levels exceed the standards, nitrifying bacteria should be added for intervention.

[0020] S2. Selection and artificial spawning of broodstock: Select female Rasch's catfish at the end of gland maturity stage IV; male low-eyed toothless catfish with sperm motility ≥80% are selected as broodstock and artificially induced to spawn by hormone injection, followed by artificial insemination.

[0021] Specifically, the selection process for broodstock is as follows: When selecting female Ragnarökle catfish, the female should have a swollen and soft abdomen, and the genital opening should be round, red, swollen, and everted. Using the "three-point palpation method," gently press the middle and posterior part of the female's abdomen with your fingers. If the eggs are free-floating and of uniform size, it indicates that the female has reached the end of stage IV maturity. Starting 15 days before induced spawning, check the softness of the female's abdomen and the free-floating state of the eggs every 3 days using the palpation method.

[0022] When selecting male low-eyed toothless giant catfish broodstock, gently press the abdomen of the male fish and collect semen (approximately 10 μL) using a capillary tube. Dilute the semen in 0.7% physiological saline (1:50). Then, place the semen sample on a glass slide and randomly select several fields of view under a 400x microscope to calculate the percentage of motile sperm. To reduce the amount of data to be tested, the color and concentration of the semen collected by capillary tube can be manually selected before microscopic observation.

[0023] Starting 15 days before spawning, male fish semen is sampled and collected, and its color is observed visually; it reaches maturity when it is milky white.

[0024] Specifically, the artificial spawning induction procedure is as follows: For female *Catfish larvae* broodstock, administer a mixture of DOM and LRH-A2 (10-15 μg / kg LRH-A2 and 5-8 mg / kg DOM by weight) via two injections at the base of the pectoral fin, with an interval of 8-10 hours. For male *Catfish larvae* broodstock, administer a single injection at the base of the pectoral fin via a mixture of LRH-A2 and 11-KT (5-10 μg / kg LRH-A2 and 0.1-0.3 mg / kg DOM by weight). After injection, the water temperature in the rearing water should be maintained at 26-28℃.

[0025] Specifically, the artificial insemination procedure is as follows: Egg collection: Two people work together, wrapping the female fish's body with sterile gauze, with the genital opening facing the sterile egg collection dish, and applying even pressure along the caudal peduncle to collect the eggs. Sperm collection: Collect semen using the live puncture method, and dilute it with an activating solution (NaCl 7.5g / L + KCl 0.2g / L + glucose 1g / L) at a 1:5 ratio. Egg pretreatment: Rinse the eggs of *Catella raspetus* using 0.1% trypsin (pH 7.4) combined with 0.05% hyaluronidase (500 IU / mL). Insemination: Mix the eggs and sperm at a 1:3 ratio, stir clockwise with a bird feather for 30 seconds, let stand for 1 minute, and then rinse three times with 0.7% physiological saline to remove excess semen. Afterwards, soak the eggs in a 0.3% talc solution (food grade, particle size 10-20μm) for 3-5 minutes to remove any adhesion. After de-adhesion, rinse three times with clean water (each time the water volume is 5 times the volume of the eggs). During artificial insemination, the temperature of various solutions is controlled at 26-28 degrees Celsius.

[0026] S3. Fertilized egg incubation management: The incubation facility uses a conical plastic tank with an 80-mesh egg-attaching net at the bottom. A circulating water system maintains the water flow within the conical tank, ensuring uniform egg suspension and preventing accumulation. Environmental parameters require strict control: water temperature stable at 26-28℃, dissolved oxygen ≥6mg / L, pH 7.0-7.5, and a consistently low-light environment (<500 lux). 24 hours before hatching, the light intensity is increased to 1000 lux to stimulate hatching. Furthermore, 450nm blue light (1000 lux) is used as the additional light source.

[0027] The hatching process is managed dynamically in stages: Cleavage stage (0-8 hours): Unfertilized eggs that sink to the bottom are removed promptly (sinking rate <5%) to prevent mold and water pollution. Blastocyst stage (8-20 hours): The water flow rate is reduced to 0.1-0.15 m / s to reduce mechanical stimulation, and the transparency of the eggs is checked regularly, removing any abnormal eggs that are cloudy or whitish. Hatching stage (50-55 hours): The water flow rate is further reduced to 0.05-0.1 m / s to avoid damage to the juvenile fish from the impact of the water flow, and the hatched juvenile fish are transferred to the nursery pond using a siphon.

[0028] S4. Juvenile fish rearing: Juvenile fish rearing requires a balance of nutrition supply, environmental control and disease prevention to ensure a smooth transition of juveniles from endogenous nutrition (yolk sac) to exogenous feeding.

[0029] The initial feeding is divided into three stages: Initial stage (1-3 days old): Feed rotifers (15-20 rotifers / ml) combined with Chlorella (5×10 mol / ml). 4 Cells / ml), promoting neural development. Transition period (4-7 days old): Gradually replace with Artemia nauplii (8-10 cells / ml), Vitamin B12 (0.02 mg / L), and Chlorella (1×10⁻⁶ cells / ml), promoting neural development. 4 cells / ml). Later stage (≥8 days old): Feed microparticle feed (particle size 150-200μm, crude protein ≥50%), add Bacillus subtilis (10 6 (CFU / g), the feeding amount is 15% of the fish's body weight, and it is fed 4 times a day.

[0030] Environmental control measures include: daily water exchange of 30% and a stepped temperature difference adjustment (each temperature change ≤1℃) to maintain dissolved oxygen ≥5mg / L and ammonia nitrogen ≤0.2mg / L. Weekly application of povidone-iodine (1g / m³) to the entire pond to kill pathogens, along with the addition of allicin (0.1%) and bile acids (0.05%) to the feed for 5 consecutive days to prevent enteritis and lipid metabolism disorders.

[0031] Phenotypic optimization should begin at 30 days of age: select individuals with a dorsal fin height / body length ≥12%, and induce melanin deposition through blue light irradiation (450nm, 6h / d) to achieve a silver-blue gradient in body color, resembling the ornamental characteristics of the Genghis Khan shark. During this stage, strong light stress should be avoided, and light intensity should be controlled between 500-800 lux.

[0032] During the development of this patented technology, the applicant conducted small-scale verification and set up comparative cases of artificial breeding of female and male *Macrobrachium raspinatum*, with the specific conditions set as follows: Example: Five-year-old female *Catfish lacherae* with an average weight of 5.5 kg, a body length of 30-35 cm, and a dorsal fin height / body length ratio of 12% were selected as parent fish; and five-year-old male *Catfish lacherae* with an average weight of 6.4 kg, a body length of 70-75 cm, and a dorsal fin length / body length ratio of 11% were selected as parent fish.

[0033] Comparative ratio: Five-year-old female Rathian catfish with an average weight of 5.5 kg, a body length of 30-35 cm, and a dorsal fin height / body length ratio of 12% were selected as parent fish; five-year-old male Rathian catfish with an average weight of 5.6 kg, a body length of 30-35 cm, and a dorsal fin length / body length ratio of 12.5% ​​were selected as parent fish.

[0034] All other aspects were consistent (60 days of intensive cultivation, 18 hours of white light daily, intermittent variable frequency pulses daily, i.e., 0.5 m / s flow rate for 30 minutes, followed by a 1 m / s flow rate for 30 minutes after a 30-minute interval; feeding amount was 2% of body weight). Experimental results showed that the hatching rate in the comparative example was approximately 55%, while the hatching rate in the example was approximately 40%. The example hatching rate was lower than the comparative example, but considering the subsequent economic value of the hybrid fish, this hatching rate is within the expected range. Regarding growth rate, 30-day-old fry were sampled and measured. The average body length in the example was 4.5 cm, and the average weight was 1.5 g, while the average body length in the comparative example was 3.8 cm, and the average weight was 1.2 g. This shows that the hybrid fry have a significant advantage in growth rate.

[0035] In the phenotypic optimization stage, a control experiment was set up. The example fish were selected juvenile fish that were exposed to blue light for 6 hours a day. The control group consisted of juvenile fish that did not pass the selection and were exposed to natural light. All other feeding conditions were the same. Their body color was evaluated at 90 days of age. In the example fish, more than 70% of the individuals showed a silver-blue gradient with a metallic sheen on their backs. In the control group, only about 5% of the individuals showed a similar gradient color.

[0036] It should be noted that, unless otherwise explicitly specified and limited, terms such as "installation," "connection," "joining," "fixing," and "setting" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this invention according to the specific circumstances.

Claims

1. A method for artificial propagation of pangasian jacks, characterized by, It comprises the following steps: S1, parent screening and cultivation, female Pseudobagrus joinkongensis and male Pseudobagrus euprystrogaster are selected as parent fish; the parent fish screened is subjected to intensive cultivation, and the intensive cultivation cycle is 30-60 days before spawning; S2, seed fish screening and artificial spawning, female Pseudobagrus joinkongensis in the late IV period of sexual gland maturity and male Pseudobagrus euprystrogaster with sperm motility of 80% or more are selected as seed fish, and artificial insemination is performed after artificial spawning by hormone injection; S3, fertilized egg hatching management, conical plastic barrels are used for hatching, a circulating water system is used to maintain the water in the conical plastic barrels in a flowing state, and the membrane-broken larvae are transferred to a rearing pond after hatching; S4, juvenile fish cultivation, juvenile fish with a dorsal fin height / body length ratio of 15% or more are screened after 30 days of juvenile fish cultivation, and phenotype optimization is performed by blue light irradiation.

2. The method for artificial propagation of pangasian latjon as claimed in claim 1 wherein, In the step S1, the female Pseudobagrus joinkongensis screening standard is 4-6 years old, the body weight is greater than or equal to (3-5) kg, the body length is greater than 30 cm, and the dorsal fin height / body length ratio is greater than or equal to 10%; the male Pseudobagrus euprystrogaster screening standard is 5-6 years old, the body weight is greater than or equal to 6 kg, the body length is greater than 70 cm, and the dorsal fin length / body length ratio is greater than or equal to 10%; the intensive cultivation cycle in the step S1 is 30-60 days before spawning, the water temperature of the water body for cultivation is 26±2℃, the dissolved oxygen is greater than or equal to 5 mg / L, and the pH is 7.0-7.5; the daily light time is not less than 14 hours, and the light source is preferably white or red LED light source; the daily pulse water flow is at least twice; the female Pseudobagrus joinkongensis feed formula is 50% animal protein, 40% plant protein, 8% fat, and 2% fiber, and the additives are astaxanthin, vitamin E, and vitamin C; the male Pseudobagrus euprystrogaster feed formula is 55% plant protein, 30% animal protein, 10% fat, and 5% fiber, and the additives are beta-glucan, bile acid, and vitamin B12, and the feeding amount is 2%-3% of the body weight.

3. The method for artificial propagation of Pseudobunocephalus affinis as claimed in claim 2, wherein, The pulse water flow in the step S1 intensive cultivation is intermittent variable frequency pulse, that is, 0.5 m / s flow rate for 15-30 min, and 1 m / s flow rate for 15-30 min after 15-30 min interval.

4. The method for artificial propagation of pangasius sanit angus as claimed in claim 1, wherein, The seed fish screening in the step S2 is specifically operated as follows: when the female Pseudobagrus joinkongensis seed fish is screened, the female fish is required to have a soft and swollen abdomen, a round and red swelling and everted genital pore, and a free ovum with uniform particle size when the finger is pressed on the middle and rear segments of the female fish abdomen, indicating that the maturity reaches the late IV period; 15 days before spawning, the male fish semen is sampled and its color is observed by visual observation, and the male Pseudobagrus euprystrogaster seed fish is selected when the semen is milky white; the male fish abdomen is pressed, the semen is collected in a capillary tube, diluted in 0.7% physiological saline, and then the semen sample is observed under a 400 times microscope to obtain the proportion of motile sperm.

5. The method for artificial propagation of pangasius labeo of claim 1, characterized by, The artificial spawning in the step S2 is specifically operated as follows: DOM and LRH-A2 mixed agents are used for the female Pseudobagrus joinkongensis seed fish, and the chest fin base is injected twice with an interval of 8-10 hours; LRH-A2 and 11-KT mixed agents are used for the male Pseudobagrus euprystrogaster seed fish, and the chest fin base is injected once.

6. The method for artificial propagation of pangasius labeo of claim 1, characterized by, The step S2 artificial insemination is specifically operated as follows: ovum collection, two people cooperate to operate, the female fish trunk is wrapped with sterile gauze, the genital orifice is towards the sterilized ovum collection dish, and the ovum is collected along the tail handle direction; sperm collection, the sperm is collected by living body puncture method, and the NaCl 7.5 g / L+KCl 0.2 g / L+glucose 1 g / L mixed solution is diluted according to 1:5; ovum pretreatment, 0.1% trypsin combined with 0.05% hyaluronidase is used to flush the clarias batrachus ovum; insemination, the ovum-sperm mixing ratio is 1:3, the poultry feather is used to stir clockwise for 30 seconds, and after standing for 1 minute, the excess sperm is removed by washing with 0.7% physiological saline for 3 times, then 0.3% talcum powder solution is used for soaking for 3-5 minutes for debonding treatment, and after debonding treatment, the water is rinsed for 3 times.

7. The method for artificial propagation of pangasius labeo as claimed in claim 1, wherein, The step S3 fertilized egg hatching management specifically includes the following processes: the 80-mesh attached egg net is arranged at the bottom of the conical plastic barrel, the water in the conical plastic barrel is maintained in a flowing state through a circulating water system, the water temperature is stabilized at 26-28 DEG C, the dissolved oxygen content is greater than or equal to 6 mg / L, the pH value is 7.0-7.5, the whole process is in a weak light environment, and the light is increased to 1000 lux at 24 hours before membrane rupture to stimulate membrane rupture.

8. The method for artificial propagation of pangasius labeo as claimed in claim 1, wherein, The step S4 juvenile fish cultivation opening bait feeding is divided into three stages: 1-3 days old, rotifer is fed in combination with chlorella; 4-7 days old, brine shrimp nauplii, vitamin B12 and green algae are used; and greater than or equal to 8 days old, microparticle feed is fed, bacillus subtilis is added, the feeding amount is 15% of the fish body weight, and the feeding is divided into 4 times per day.

9. The method for artificial propagation of pangasius labeo as claimed in claim 1, wherein, The phenotype optimization in the step S4 juvenile fish cultivation is specifically operated as follows: the back fin height / body length is greater than or equal to 15%, and the 450 nm blue light is irradiated for at least 6 hours per day.