High-survival-rate cultivation method for cephalopholis sonnerati fry

Abstract

A high-survival-rate cultivation method for Cephalopholis sonnerati fry, relating to the technical field of marine fish cultivation. The cultivation method comprises the following steps: (1) regulation of a light source environment: during growth of Cephalopholis sonnerati fry, using a blue light source having a wavelength range of 420-450 nm to irradiate for 60-75 d; (2) modification of a water bottom environment: laying a layer of artificial material on a water bottom, introducing water having a salinity of 30-35‰ as cultivation water, and using an aeration pump for continuous aeration; and (3) alternate administration of feed A and feed B: first administering feed A for 8-10 d, then administering feed B for 8-10 d, and continuing alternate administration until the fry reach a juvenile stage, wherein feed A is prepared by adding Daphniphyllum calycinum Benth. to a base feed, and feed B is prepared by adding loperamide to the base feed. By means of three parallel measures of light source regulation + water bottom modification + alternate administration, it is beneficial to achieving high survival rate of Cephalopholis sonnerati fry, thereby improving economic benefits.

Description

A method for raising red snapper fry with high survival rate

[0001] This application requests priority to the prior invention patent application with application number 202411416462.1, application date 2024-10-11, entitled "A method for cultivating red nine-spined bass fry with high survival rate". Technical Field

[0002] This application relates to the field of marine fish aquaculture technology, and in particular to a method for cultivating red snapper fry with a high survival rate. Background Technology

[0003] The red snapper (Cephalopholis sonnerati), commonly known as the reticulated grouper, is a species of fish belonging to the genus Cephalophorus in the family Serranidae, order Perciformes, suborder Perciformes, class Actinopterygii. It has a red body with dark brown reticulated markings on the head and front of the back, lacking spots and stripes. It has no canine teeth in the middle of its lower jaw, and its anal fin has 3 spines and 9 rays. The posterior margin of its caudal fin is rounded and lacks stripes. It typically inhabits shallow, rocky seabeds, feeding on invertebrates and fish, and is a common fish found in tropical coral reefs and along coastlines. Red-spined perch, a high-end edible fish, is highly favored by consumers. However, wild red-spined perch populations have declined sharply due to overfishing. Furthermore, artificial breeding technology for red-spined perch in my country is still immature, and research on artificial breeding methods for fry is limited. This hinders large-scale artificial breeding of red-spined perch fry, particularly during the breeding process, where unstable feeding rates lead to poor growth, development, and reproductive capacity, resulting in low survival rates and an inability to meet market demand. Therefore, providing stable, high-survival-rate breeding methods for red-spined perch fry to achieve large-scale artificial breeding has become a crucial area that urgently needs to be addressed in the aquaculture industry. Summary of the Invention

[0004] In view of the above-mentioned deficiencies in the prior art, this application proposes a method for cultivating red snapper fry with a high survival rate, so as to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, this application provides the following technical solution:

[0006] A method for cultivating red snapper fry with high survival rate, the method comprising the following steps:

[0007] (1) Controlling the light source environment: During the growth of red sea bass fry, use a blue light source with a wavelength range of 420-450nm to irradiate for 60-75 days;

[0008] (2) Aquatic environment modification: Lay a layer of artificial material on the bottom of the water, introduce water with a salinity of 30-35‰ as aquaculture water, and use oxygen pumps to aerate all day long;

[0009] (3) Alternate feeding of feed A and feed B: feed feed A for 8-10 days, then feed feed B for 8-10 days, and alternate feeding until the juvenile stage; feed A is prepared by adding Daphniphyllum calycinum Benth., a shrub of Daphniphyllum genus in the family Daphniphyllum, and feed B is prepared by adding loperamide to the basic feed;

[0010] The artificial material, by weight, includes 12-20 parts of oyster shell powder-kaolin modified material, 10-15 parts of sodium silicate, 5-8 parts of superphosphate, and 4-10 parts of Clostridium butyricum metabolites.

[0011] Preferably, the stocking density of red snapper fry is 100–200 fish / m². 2 .

[0012] Preferably, the blue light source is placed 80-100cm above the aquaculture water.

[0013] Preferably, the light intensity of the blue light source in step (1) is 100-150 lux, and the light period is 17L:7D.

[0014] Preferably, the artificial material in step (2) comprises, by weight, 15 parts oyster shell powder-kaolin modified material, 12 parts sodium silicate, 6 parts superphosphate, and 5 parts Clostridium butyricum metabolites.

[0015] Preferably, Clostridium butyricum was purchased from Shanghai Yiyan Biotechnology Co., Ltd.

[0016] Preferably, the thickness of the artificial material laid in step (2) is 3 to 6 cm.

[0017] Preferably, the preparation method of the oyster shell powder-kaolin modified material in step (2) is as follows: by weight, 2-6 parts of oyster shell powder and 5-10 parts of kaolin are added to 10-20 parts of anhydrous ethanol, and after standing for 10-15 minutes, 1-3 parts of hexadecyltrimethoxysilane are added. The mixture is heated to 75-82°C while stirring, followed by ultrasonic vibration for 10-20 minutes, filtered, washed with water, and dried to obtain the modified material.

[0018] Preferably, the preparation method of the butyric acid Clostridium metabolites in step (2) is as follows: after activation, butyric acid Clostridium is inoculated into a reinforced Clostridium culture medium and anaerobic static culture at 30-35℃ for 12-16h to obtain bacterial solution; 1% of the bacterial solution is inoculated into LB liquid culture medium and cultured at 28-32℃ and 220-260rpm for 5-8h with shaking; after centrifugation, the supernatant is collected to obtain the butyric acid Clostridium metabolites.

[0019] Preferably, the enhanced Clostridium perfringens medium (RCM medium) consists of: 3g yeast extract, 10g beef extract, 5g sodium chloride, 3g sodium acetate trihydrate, 0.5g cysteine ​​hydrochloride, 0.2ml 0.5% methylene blue, and 1000ml distilled water; adjust the pH to 7.1±0.1, and sterilize at 115℃ for 20min for later use.

[0020] Preferably, LB (Luria-Bertani) liquid culture medium (lysozyme broth medium) consists of 10g peptone, 5g yeast powder, and 10g NaCl, with water added to 1000ml. The pH is adjusted to 7.2-7.4 using NaOH or HCl, and the medium is sterilized at 115℃ for 30 minutes before use.

[0021] Preferably, the preparation method of the artificial material in step (2) is as follows: oyster shell powder-kaolin modified material, sodium silicate and superphosphate are mixed; 2 to 5 times the volume of anhydrous ethanol is added to the mixture, and the mixture is subjected to a first ultrasonic vibration and ball milling. Then, Clostridium butyricum metabolites are added, and a second ultrasonic vibration is performed to obtain the artificial material.

[0022] Preferably, the first ultrasonic oscillation frequency is 20-25 kHz and the duration is 12-20 min; the second ultrasonic oscillation frequency is 30-40 kHz and the duration is 5-10 min.

[0023] Preferably, in step (3), the amount of ox-ear fungus and loperamide added to feed A and feed B is 5-9 wt%, respectively, and the feeding frequency is 3 times / day, with a daily feeding amount of 3-6% of the fish fry's body weight.

[0024] Preferably, the basic feed consists of 35% white fish meal, 15% soybean meal, 4% dried shrimp, 4% soybean lecithin, 30.2% protein powder, 2.68% fish oil, 1.32% soybean oil, 6.5% starch, 1% choline, 0.1% Vino fish multivitamins, 0.1% Vino mycotoxin multiminerals, and 0.1% Vino compound probiotics.

[0025] Compared with the prior art, the beneficial effects of this application are:

[0026] (1) This application uses a blue light source with a specific spectrum to regulate the light environment of red nine-spined perch fry, which meets the light requirements of red nine-spined perch, improves the problem of uneven size of red nine-spined perch during growth, and makes the growth rate of red nine-spined perch not much different in the same time period, effectively improving the economic benefits of red nine-spined perch farming.

[0027] (2) The underwater artificial material is prepared using oyster shell powder-kaolin modified material, sodium silicate, superphosphate, and Clostridium butyricum metabolites. Oyster shell powder and kaolin itself have adsorption properties, which can adsorb and remove phosphate and ammonia nitrogen in the water. Since the modified material is placed at the bottom of the water, the increased permeability will reduce its adsorption of harmful substances in the water. However, this technical solution uses hexadecyltrimethoxysilane as a modifier to hydrophobically modify oyster shell powder-kaolin to improve adsorption. Together with sodium silicate, superphosphate, and Clostridium butyricum metabolites, it can purify the water, improve the aquatic environment, and help improve the survival rate of red snapper fry. In addition, Clostridium butyricum metabolites can promote the growth of beneficial bacteria in the intestines of fish fry, inhibit harmful bacteria, correct bacterial flora disorder, reduce toxin production, regulate the intestinal microecological balance of fish, thereby shortening the breeding time of red snapper fry and reducing breeding costs.

[0028] (3) In this application, feed A and feed B are fed alternately. Chinese medicine is added to feed A and Western medicine is added to feed B. The Chinese medicine ingredients are compatible with the activity of the fish body and can be fully absorbed and utilized by the body. No drug residues will be produced in the body, thus avoiding the problem of drug residues. Western medicine has a fast onset of action and can quickly promote the fish body to digest food and absorb nutrients. The combined use of Chinese medicine and Western medicine can produce a synergistic effect, regulate the body's immunity, and improve the problem of low yield of red sea bass. Attached Figure Description

[0029] Figure 1 is a schematic diagram of the hatching period of the red snapper;

[0030] Figure 2 is a schematic diagram of the juvenile Red Nine-Spined Bass.

[0031] Figure 3 is a schematic diagram of the adult stage of the red snapper. Detailed Implementation

[0032] The technical solution of this application will be further described in detail below with reference to specific embodiments.

[0033] Example 1

[0034] According to the existing indoor breeding and aquaculture methods, the water quality indicators are controlled as follows: pH 7.5-7.9, dissolved oxygen ≥5mg / L, ammonia nitrogen ≤0.5mg / L, and nitrite ≤0.05mg / L; red sea bass fry are bred according to the following control methods.

[0035] A method for cultivating red snapper fry with high survival rate, the method comprising the following steps:

[0036] (1) Controlling the light source environment: During the growth of red sea bass fry, a blue light source with a wavelength range of 420nm and a light intensity of 100lux was used to irradiate for 60 days, with a photoperiod of 17L:7D. The blue light source was placed 80cm above the culture water.

[0037] (2) Aquatic environment modification: Lay a layer of artificial material with a thickness of 3-6cm on the bottom of the water, introduce water with a salinity of 30‰ as aquaculture water, and use an oxygen pump to aerate all day long;

[0038] (3) Alternate feeding of feed A and feed B: Feed A for 8 days first, then feed B for 8 days, feeding 3 times / day at 09:00, 13:00 and 18:00 respectively, with a daily feeding amount of 3% of the fish fry's body weight, and alternate feeding until the juvenile stage; feed A is prepared by adding 5wt% of Acer buergerianum to the basic feed, and feed B is prepared by adding 5wt% of loperamide to the basic feed;

[0039] The artificial material, by weight, comprises 12 parts oyster shell powder-kaolin modified material, 10 parts sodium silicate, 5 parts superphosphate, and 4 parts Clostridium butyricum metabolites.

[0040] The preparation method of the oyster shell powder-kaolin modified material in step (2) is as follows: by weight, 2 parts of oyster shell powder and 5 parts of kaolin are added to 10 parts of anhydrous ethanol, and after standing for 10 minutes, 1 part of hexadecyltrimethoxysilane is added. While stirring, the mixture is heated to 75°C, followed by ultrasonic vibration for 10 minutes. The mixture is then filtered, washed with water, and dried to obtain the modified material.

[0041] The preparation method of Clostridium butyricum metabolites is as follows: Clostridium butyricum is activated and inoculated into a Clostridium-enriched culture medium, and anaerobic static culture is carried out at 30℃ for 12h to obtain bacterial solution; 1% of the bacterial solution is inoculated into LB liquid culture medium, and cultured at 28℃ and 220rpm for 5h with shaking, centrifugation is performed, and the supernatant is collected to obtain Clostridium butyricum metabolites.

[0042] The preparation method of the artificial material in step (2) is as follows: oyster shell powder-kaolin modified material, sodium silicate and superphosphate are mixed to obtain a mixture; 2 times the volume of anhydrous ethanol is added to the mixture, and ultrasonic vibration at a frequency of 20kHz is performed for 12 minutes. The mixture is then fully ball-milled, and Clostridium butyricum metabolites are added. The mixture is then ultrasonically vibrated at a frequency of 30kHz for 5 minutes to obtain the artificial material.

[0043] Example 2

[0044] According to the existing indoor breeding and aquaculture methods, the water quality indicators are controlled as follows: pH 7.5-7.9, dissolved oxygen ≥5mg / L, ammonia nitrogen ≤0.5mg / L, and nitrite ≤0.05mg / L; red sea bass fry are bred according to the following control methods.

[0045] A method for cultivating red snapper fry with high survival rate, the method comprising the following steps:

[0046] (1) Controlling the light source environment: During the growth of red sea bass fry, a blue light source with a wavelength range of 450nm and a light intensity of 150lux was used to irradiate for 75 days, with a photoperiod of 17L:7D. The blue light source was placed 100cm above the culture water.

[0047] (2) Aquatic environment modification: Lay a layer of artificial material with a thickness of 3-6cm on the bottom of the water, introduce water with a salinity of 35‰ as the aquaculture water, and use an oxygen pump to aerate all day long;

[0048] (3) Alternate feeding of feed A and feed B: Feed A for 10 days first, then feed B for 10 days, feeding 3 times / day at 09:00, 13:00 and 18:00 respectively, with a daily feeding amount of 6% of the fish fry's body weight, and alternate feeding until the juvenile stage; feed A is prepared by adding 9wt% of Acer buergerianum to the basic feed, and feed B is prepared by adding 9wt% of loperamide to the basic feed;

[0049] The artificial material, by weight, includes 20 parts oyster shell powder-kaolin modified material, 15 parts sodium silicate, 8 parts superphosphate, and 10 parts Clostridium butyricum metabolites.

[0050] The preparation method of the oyster shell powder-kaolin modified material in step (2) is as follows: by weight, 6 parts of oyster shell powder and 10 parts of kaolin are added to 20 parts of anhydrous ethanol, and after standing for 15 minutes, 3 parts of hexadecyltrimethoxysilane are added. While stirring, the mixture is heated to 82°C, followed by ultrasonic vibration for 20 minutes. The mixture is then filtered, washed with water, and dried to obtain the modified material.

[0051] The preparation method of Clostridium butyricum metabolites is as follows: Clostridium butyricum is activated and inoculated into a Clostridium-enriched culture medium, and anaerobic static culture is carried out at 35℃ for 16h to obtain bacterial solution; 1% of the bacterial solution is inoculated into LB liquid culture medium, and cultured at 32℃ and 260rpm for 8h with shaking, centrifugation is performed, and the supernatant is collected to obtain Clostridium butyricum metabolites.

[0052] The preparation method of the artificial material in step (2) is as follows: oyster shell powder-kaolin modified material, sodium silicate and superphosphate are mixed to obtain a mixture; 5 times the volume of anhydrous ethanol is added to the mixture, and the mixture is subjected to ultrasonic vibration at a frequency of 25kHz for 20 minutes, and then fully ball-milled. Clostridium butyricum metabolites are added, and the mixture is subjected to ultrasonic vibration at a frequency of 40kHz for 10 minutes to obtain the artificial material.

[0053] Example 3

[0054] According to the existing indoor breeding and aquaculture methods, the water quality indicators are controlled as follows: pH 7.5-7.9, dissolved oxygen ≥5mg / L, ammonia nitrogen ≤0.5mg / L, and nitrite ≤0.05mg / L; red sea bass fry are bred according to the following control methods.

[0055] A method for cultivating red snapper fry with high survival rate, the method comprising the following steps:

[0056] (1) Controlling the light source environment: During the growth of red sea bass fry, a blue light source with a wavelength range of 430nm and a light intensity of 120lux was used to irradiate for 70 days, with a photoperiod of 17L:7D; the blue light source was placed 90cm above the culture water.

[0057] (2) Aquatic environment modification: Lay a layer of artificial material with a thickness of 3-6cm on the bottom of the water, introduce water with a salinity of 32‰ as the aquaculture water, and use an oxygen pump to aerate all day long;

[0058] (3) Alternate feeding of feed A and feed B: Feed A for 9 days first, then feed B for 9 days, feeding 3 times / day at 09:00, 13:00 and 18:00 respectively, with a daily feeding amount of 5% of the fish fry's body weight, and alternate feeding until the juvenile stage; feed A is prepared by adding 7wt% of Acer palmatum to the basic feed, and feed B is prepared by adding 7wt% of loperamide to the basic feed;

[0059] The artificial material, by weight, comprises 15 parts oyster shell powder-kaolin modified material, 12 parts sodium silicate, 6 parts superphosphate, and 5 parts Clostridium butyricum metabolites.

[0060] The preparation method of the oyster shell powder-kaolin modified material in step (2) is as follows: by weight, 5 parts of oyster shell powder and 7 parts of kaolin are added to 15 parts of anhydrous ethanol, and after standing for 13 minutes, 2 parts of hexadecyltrimethoxysilane are added. While stirring, the mixture is heated to 80°C, followed by ultrasonic vibration for 15 minutes. The mixture is then filtered, washed with water, and dried to obtain the modified material.

[0061] The preparation method of Clostridium butyricum metabolites is as follows: Clostridium butyricum is activated and inoculated into a Clostridium-enriched culture medium, and anaerobic static culture is carried out at 32℃ for 15h to obtain bacterial solution; 1% of the bacterial solution is inoculated into LB liquid culture medium, and cultured at 30℃ and 250rpm for 7h with shaking, centrifugation is performed, and the supernatant is collected to obtain Clostridium butyricum metabolites.

[0062] The preparation method of the artificial material in step (2) is as follows: oyster shell powder-kaolin modified material, sodium silicate and superphosphate are mixed to obtain a mixture; 4 times the volume of anhydrous ethanol is added to the mixture, and the mixture is subjected to ultrasonic vibration at a frequency of 22kHz for 18 minutes for the first time, and then fully ball-milled. Then, Clostridium butyricum metabolites are added, and the mixture is subjected to ultrasonic vibration at a frequency of 35kHz for 9 minutes for the second time to obtain the artificial material.

[0063] Comparative Example 1

[0064] The difference between this comparative example and Example 3 is that the blue light source is replaced with a white LED light, while the other components and steps remain unchanged.

[0065] According to the existing indoor breeding and aquaculture methods, the water quality indicators are controlled as follows: pH 7.5-7.9, dissolved oxygen ≥5mg / L, ammonia nitrogen ≤0.5mg / L, and nitrite ≤0.05mg / L; red sea bass fry are bred according to the following control methods.

[0066] A method for cultivating red snapper fry with high survival rate, the method comprising the following steps:

[0067] (1) Controlling the light source environment: During the growth of red sea bass fry, a white light source with a wavelength range of 450nm and a light intensity of 120lux was used to irradiate for 70 days, with a photoperiod of 17L:7D; the white light source was placed 90cm above the culture water.

[0068] (2) Aquatic environment modification: Lay a layer of artificial material with a thickness of 3-6cm on the bottom of the water, introduce water with a salinity of 32‰ as the aquaculture water, and use an oxygen pump to aerate all day long;

[0069] (3) Alternate feeding of feed A and feed B: Feed A for 9 days first, then feed B for 9 days, feeding 3 times / day at 09:00, 13:00 and 18:00 respectively, with a daily feeding amount of 5% of the fish fry's body weight, and alternate feeding until the juvenile stage; feed A is prepared by adding 7wt% of Acer palmatum to the basic feed, and feed B is prepared by adding 7wt% of loperamide to the basic feed;

[0070] The artificial material, by weight, comprises 15 parts oyster shell powder-kaolin modified material, 12 parts sodium silicate, 6 parts superphosphate, and 5 parts Clostridium butyricum metabolites.

[0071] The preparation method of the oyster shell powder-kaolin modified material in step (2) is as follows: by weight, 5 parts of oyster shell powder and 7 parts of kaolin are added to 15 parts of anhydrous ethanol, and after standing for 13 minutes, 2 parts of hexadecyltrimethoxysilane are added. While stirring, the mixture is heated to 80°C, followed by ultrasonic vibration for 15 minutes. The mixture is then filtered, washed with water, and dried to obtain the modified material.

[0072] The preparation method of Clostridium butyricum metabolites is as follows: Clostridium butyricum is activated and inoculated into a Clostridium-enriched culture medium, and anaerobic static culture is carried out at 32℃ for 15h to obtain bacterial solution; 1% of the bacterial solution is inoculated into LB liquid culture medium, and cultured at 30℃ and 250rpm for 7h with shaking, centrifugation is performed, and the supernatant is collected to obtain Clostridium butyricum metabolites.

[0073] The preparation method of the artificial material in step (2) is as follows: oyster shell powder-kaolin modified material, sodium silicate and superphosphate are mixed to obtain a mixture; 4 times the volume of anhydrous ethanol is added to the mixture, and the mixture is subjected to ultrasonic vibration at a frequency of 22kHz for 18 minutes for the first time, and then fully ball-milled. Then, Clostridium butyricum metabolites are added, and the mixture is subjected to ultrasonic vibration at a frequency of 35kHz for 9 minutes for the second time to obtain the artificial material.

[0074] Comparative Example 2

[0075] The difference between this comparative example and Example 3 is that no artificial materials are laid, while the other components and steps remain unchanged.

[0076] According to the existing indoor breeding and aquaculture methods, the water quality indicators are controlled as follows: pH 7.5-7.9, dissolved oxygen ≥5mg / L, ammonia nitrogen ≤0.5mg / L, and nitrite ≤0.05mg / L; red sea bass fry are bred according to the following control methods.

[0077] A method for cultivating red snapper fry with high survival rate, the method comprising the following steps:

[0078] (1) Controlling the light source environment: During the growth of red sea bass fry, a blue light source with a wavelength range of 430nm and a light intensity of 120lux was used to irradiate for 70 days, with a photoperiod of 17L:7D; the blue light source was placed 90cm above the culture water.

[0079] (2) Alternate feeding of feed A and feed B: feed A for 9 days first, then feed B for 9 days, feeding 3 times / day at 09:00, 13:00 and 18:00 respectively, with a daily feeding amount of 5% of the fish fry's body weight, and alternate feeding until the juvenile stage; feed A is prepared by adding 7wt% of Acer buergerianum to the basic feed, and feed B is prepared by adding 7wt% of loperamide to the basic feed.

[0080] Comparative Example 3

[0081] The difference between this comparative example and Example 3 is that the oyster shell powder-kaolin in the artificial material is not modified, while the other components and steps remain unchanged.

[0082] According to the existing indoor breeding and aquaculture methods, the water quality indicators are controlled as follows: pH 7.5-7.9, dissolved oxygen ≥5mg / L, ammonia nitrogen ≤0.5mg / L, and nitrite ≤0.05mg / L; red sea bass fry are bred according to the following control methods.

[0083] A method for cultivating red snapper fry with high survival rate, the method comprising the following steps:

[0084] (1) Controlling the light source environment: During the growth of red sea bass fry, a blue light source with a wavelength range of 430nm and a light intensity of 120lux was used to irradiate for 70 days, with a photoperiod of 17L:7D; the blue light source was placed 90cm above the culture water.

[0085] (2) Aquatic environment modification: Lay a layer of artificial material with a thickness of 3-6cm on the bottom of the water, introduce water with a salinity of 32‰ as the aquaculture water, and use an oxygen pump to aerate all day long;

[0086] (3) Alternate feeding of feed A and feed B: Feed A for 9 days first, then feed B for 9 days, feeding 3 times / day at 09:00, 13:00 and 18:00 respectively, with a daily feeding amount of 5% of the fish fry's body weight, and alternate feeding until the juvenile stage; feed A is prepared by adding 7wt% of Acer palmatum to the basic feed, and feed B is prepared by adding 7wt% of loperamide to the basic feed;

[0087] The artificial material, by weight, comprises 15 parts oyster shell powder-kaolin mixture, 12 parts sodium silicate, 6 parts superphosphate, and 5 parts Clostridium butyricum metabolites.

[0088] The preparation method of Clostridium butyricum metabolites is as follows: Clostridium butyricum is activated and inoculated into a Clostridium-enriched culture medium, and anaerobic static culture is carried out at 32℃ for 15h to obtain bacterial solution; 1% of the bacterial solution is inoculated into LB liquid culture medium, and cultured at 30℃ and 250rpm for 7h with shaking, centrifugation is performed, and the supernatant is collected to obtain Clostridium butyricum metabolites.

[0089] The preparation method of the artificial material in step (2) is as follows: mix oyster shell powder-kaolin mixed material, sodium silicate and superphosphate to obtain a mixture; add 4 times the volume of anhydrous ethanol to the mixture, perform the first ultrasonic oscillation at a frequency of 22kHz for 18min, ball mill thoroughly, then add Clostridium butyricum metabolites, and perform the second ultrasonic oscillation at a frequency of 35kHz for 9min to obtain the artificial material.

[0090] Comparative Example 4

[0091] The difference between this comparative example and Example 3 is that the butyric acid bacteria metabolites in the artificial material are replaced with lactic acid bacteria metabolites, while the other components and steps remain unchanged.

[0092] According to the existing indoor breeding and aquaculture methods, the water quality indicators are controlled as follows: pH 7.5-7.9, dissolved oxygen ≥5mg / L, ammonia nitrogen ≤0.5mg / L, and nitrite ≤0.05mg / L; red sea bass fry are bred according to the following control methods.

[0093] A method for cultivating red snapper fry with high survival rate, the method comprising the following steps:

[0094] (1) Controlling the light source environment: During the growth of red sea bass fry, a blue light source with a wavelength range of 430nm and a light intensity of 120lux was used to irradiate for 70 days, with a photoperiod of 17L:7D; the blue light source was placed 90cm above the culture water.

[0095] (2) Aquatic environment modification: Lay a layer of artificial material with a thickness of 3-6cm on the bottom of the water, introduce water with a salinity of 32‰ as the aquaculture water, and use an oxygen pump to aerate all day long;

[0096] (3) Alternate feeding of feed A and feed B: Feed A for 9 days first, then feed B for 9 days, feeding 3 times / day at 09:00, 13:00 and 18:00 respectively, with a daily feeding amount of 5% of the fish fry's body weight, and alternate feeding until the juvenile stage; feed A is prepared by adding 7wt% of Acer palmatum to the basic feed, and feed B is prepared by adding 7wt% of loperamide to the basic feed;

[0097] The artificial material, by weight, includes 15 parts oyster shell powder-kaolin modified material, 12 parts sodium silicate, 6 parts superphosphate, and 5 parts lactic acid bacteria metabolites.

[0098] The preparation method of the oyster shell powder-kaolin modified material in step (2) is as follows: by weight, 5 parts of oyster shell powder and 7 parts of kaolin are added to 15 parts of anhydrous ethanol, and after standing for 13 minutes, 2 parts of hexadecyltrimethoxysilane are added. While stirring, the mixture is heated to 80°C, followed by ultrasonic vibration for 15 minutes. The mixture is then filtered, washed with water, and dried to obtain the modified material.

[0099] The preparation method of lactic acid bacteria metabolites is as follows: After activation, lactic acid bacteria are inoculated into agar medium and anaerobic static culture at 32℃ for 15h to obtain bacterial solution; 1% of bacterial solution is inoculated into LB liquid medium and cultured at 30℃ and 250rpm for 7h with shaking, centrifugation is performed, and the supernatant is collected to obtain lactic acid bacteria metabolites.

[0100] The preparation method of the artificial material in step (2) is as follows: oyster shell powder-kaolin modified material, sodium silicate and superphosphate are mixed to obtain a mixture; 4 times the volume of anhydrous ethanol is added to the mixture, and the mixture is subjected to ultrasonic vibration at a frequency of 22kHz for 18 minutes for the first time, and then fully ball-milled. Then, lactic acid bacteria metabolites are added, and the mixture is subjected to ultrasonic vibration at a frequency of 35kHz for 9 minutes for the second time to obtain the artificial material.

[0101] Comparative Example 5

[0102] The difference between this comparative example and Example 3 is that the fish fry feed is the basic feed, without the addition of ox-ear fungus and loperamide, while the other ingredients and steps remain unchanged.

[0103] According to the existing indoor breeding and aquaculture methods, the water quality indicators are controlled as follows: pH 7.5-7.9, dissolved oxygen ≥5mg / L, ammonia nitrogen ≤0.5mg / L, and nitrite ≤0.05mg / L; red sea bass fry are bred according to the following control methods.

[0104] A method for cultivating red snapper fry with high survival rate, the method comprising the following steps:

[0105] (1) Controlling the light source environment: During the growth of red sea bass fry, a blue light source with a wavelength range of 430nm and a light intensity of 120lux was used to irradiate for 70 days, with a photoperiod of 17L:7D; the blue light source was placed 90cm above the culture water.

[0106] (2) Aquatic environment modification: Lay a layer of artificial material with a thickness of 3-6cm on the bottom of the water, introduce water with a salinity of 32‰ as the aquaculture water, and use an oxygen pump to aerate all day long;

[0107] (3) Feeding basic feed: Feed 3 times / day, at 09:00, 13:00 and 18:00 respectively, with a daily feed amount of 5% of the fish fry's body weight, and feed alternately until the juvenile stage; the basic feed consists of 35% white fish meal, 15% soybean meal, 4% dried shrimp, 4% soybean lecithin, 30.2% protein powder, 2.68% fish oil, 1.32% soybean oil, 6.5% starch, 1% choline, 0.1% Vino fish multivitamins, 0.1% Vino mycotoxin multimineral, and 0.1% Vino compound probiotics;

[0108] The artificial material, by weight, comprises 15 parts oyster shell powder-kaolin modified material, 12 parts sodium silicate, 6 parts superphosphate, and 5 parts Clostridium butyricum metabolites.

[0109] The preparation method of the oyster shell powder-kaolin modified material in step (2) is as follows: by weight, 5 parts of oyster shell powder and 7 parts of kaolin are added to 15 parts of anhydrous ethanol, and after standing for 13 minutes, 2 parts of hexadecyltrimethoxysilane are added. While stirring, the mixture is heated to 80°C, followed by ultrasonic vibration for 15 minutes. The mixture is then filtered, washed with water, and dried to obtain the modified material.

[0110] The preparation method of the artificial material in step (2) is as follows: oyster shell powder-kaolin modified material, sodium silicate and superphosphate are mixed to obtain a mixture; 4 times the volume of anhydrous ethanol is added to the mixture, and the mixture is subjected to ultrasonic vibration at a frequency of 22kHz for 18 minutes for the first time, and then fully ball-milled. Then, Clostridium butyricum metabolites are added, and the mixture is subjected to ultrasonic vibration at a frequency of 35kHz for 9 minutes for the second time to obtain the artificial material.

[0111] The method for preparing the butyric acid clostridium metabolites is as follows: after activation, butyric acid clostridium is inoculated into a reinforced clostridium culture medium and anaerobic static culture at 32°C for 15 hours to obtain bacterial suspension; 1% of the bacterial suspension is inoculated into LB liquid culture medium and cultured at 30°C and 250 rpm for 7 hours by shaking, centrifugation is performed, and the supernatant is collected to obtain the butyric acid clostridium metabolites.

[0112] Comparative Example 6

[0113] The difference between this comparative example and Example 3 is that the fish fry feed is not alternated, while the other ingredients and steps remain unchanged.

[0114] According to the existing indoor breeding and aquaculture methods, the water quality indicators are controlled as follows: pH 7.5-7.9, dissolved oxygen ≥5mg / L, ammonia nitrogen ≤0.5mg / L, and nitrite ≤0.05mg / L; red sea bass fry are bred according to the following control methods.

[0115] A method for cultivating red snapper fry with high survival rate, the method comprising the following steps:

[0116] (1) Controlling the light source environment: During the growth of red sea bass fry, a blue light source with a wavelength range of 430nm and a light intensity of 120lux was used to irradiate for 70 days, with a photoperiod of 17L:7D; the blue light source was placed 90cm above the culture water.

[0117] (2) Aquatic environment modification: Lay a layer of artificial material with a thickness of 3-6cm on the bottom of the water, introduce water with a salinity of 32‰ as the aquaculture water, and use an oxygen pump to aerate all day long;

[0118] (3) Feeding a mixture of feed A and feed B: Feeding 3 times / day, at 09:00, 13:00 and 18:00 respectively, with a daily feed amount of 5% of the fish fry's body weight, and feeding alternately until the juvenile stage; The mixed feed is prepared by adding 7wt% of Acer buergerianum and 7wt% of loperamide to the basic feed.

[0119] The artificial material, by weight, comprises 15 parts oyster shell powder-kaolin modified material, 12 parts sodium silicate, 6 parts superphosphate, and 5 parts Clostridium butyricum metabolites.

[0120] The preparation method of the oyster shell powder-kaolin modified material in step (2) is as follows: by weight, 5 parts of oyster shell powder and 7 parts of kaolin are added to 15 parts of anhydrous ethanol, and after standing for 13 minutes, 2 parts of hexadecyltrimethoxysilane are added. While stirring, the mixture is heated to 80°C, followed by ultrasonic vibration for 15 minutes. The mixture is then filtered, washed with water, and dried to obtain the modified material.

[0121] The preparation method of Clostridium butyricum metabolites is as follows: Clostridium butyricum is activated and inoculated into a Clostridium-enriched culture medium, and anaerobic static culture is carried out at 32℃ for 15h to obtain bacterial solution; 1% of the bacterial solution is inoculated into LB liquid culture medium, and cultured at 30℃ and 250rpm for 7h with shaking, centrifugation is performed, and the supernatant is collected to obtain Clostridium butyricum metabolites.

[0122] The preparation method of the artificial material in step (2) is as follows: oyster shell powder-kaolin modified material, sodium silicate and superphosphate are mixed to obtain a mixture; 4 times the volume of anhydrous ethanol is added to the mixture, and the mixture is subjected to ultrasonic vibration at a frequency of 22kHz for 18 minutes for the first time, and then fully ball-milled. Then, Clostridium butyricum metabolites are added, and the mixture is subjected to ultrasonic vibration at a frequency of 35kHz for 9 minutes for the second time to obtain the artificial material.

[0123] Experiment Example 1: Survival rate of fish fry

[0124] Red snapper fry were cultured using the methods of Examples 1-3 and Comparative Examples 1-6, respectively. The number of fish stocked and harvested were counted, and the survival rate was calculated as follows: Survival rate = number of fish harvested / number of fish stocked. The results are shown in Table 1.

[0125] Table 1 Survival rate of fish fry rearing

[0126] As shown in Table 1, the survival rate of red snapper fry cultured using the methods in Examples 1-3 was high (as shown in Figures 1 and 2), reaching 98.0% or higher. Comparing Example 3 with Comparative Example 1, it can be seen that the survival rate of the fry decreased after switching to a white light source, indicating that the blue light source used in this technical solution can better improve the survival rate of the fry. The blue light source can simulate the light conditions in the natural environment, providing a comfortable and safe growth environment for the fish, promoting the growth of the fry, and making the muscle cells grow stronger. Comparing Example 3 with Comparative Examples 2-4, it can be seen that the survival rate of red snapper fry significantly decreased in Comparative Example 2 (no artificial material was used), in Comparative Example 3 (the oyster shell powder-kaolin material was not modified), and in Comparative Example 4 (lactic acid bacteria were used instead of butyric acid bacteria). This indicates that the metabolites of Clostridium butyricum have a specific effect on improving the immunity of red snapper fry. Metabolites obtained by replacing the fry with probiotic strains of similar function show significantly lower levels of immunity enhancement. The oyster shell powder-kaolin modified material, in synergy with sodium silicate, superphosphate, and Clostridium butyricum metabolites, purifies the water, improves the aquatic environment, and is beneficial for increasing the survival rate of red snapper fry. If the oyster shell powder-kaolin is not modified, the adsorption effect of the oyster shell powder-kaolin mixture on harmful substances in the water will be reduced, easily causing water turbidity, reducing oxygen content, and thus decreasing the survival rate of the fry. Comparison of Example 3 with Comparative Examples 5 and 6 shows that adding *Aristolochia debilis* and loperamide to ordinary feed is beneficial for increasing the fry's feed intake and strengthening their constitution. Through the synergistic effect of traditional Chinese medicine and Western medicine, the fish's immune capacity is enhanced, improving the survival rate after rearing.

[0127] Experiment Example 2: Weight and Total Length Test

[0128] The weight and total length of red snapper fry were measured during the rearing period to evaluate their growth performance. Fifty red snapper fry were randomly selected, and their weight and total length were measured at 30, 60, and 90 days using a weighing scale with an accuracy of 0.01 g and a ruler with an accuracy of 0.01 mm. The conventional rearing method was based on application number CN202311642700.6, "A Method for Factory-Scale Artificial Breeding of Red Snapper Fry," and the resulting fry served as a control group. The results are shown in Table 2.

[0129] Table 2 Results of Weight and Total Length Tests

[0130] As shown in Table 2, the method for raising juvenile nine-spined perch fry using the method of this application can increase the weight and total length of the fish and increase the growth rate faster compared with conventional farming. Furthermore, as shown in the data from Examples 1 to 3, the juveniles raised using the method of this application are uniform in size. Juvenile juveniles are more consistent in feeding and growth rate, which reduces competition and survival pressure caused by individual differences, thereby improving the overall survival rate.

[0131] The above description is merely a preferred embodiment of this application and is not intended to limit this application. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this application should be included within the protection scope of this application.

Claims

1. A method for cultivating red snapper fry with a high survival rate, characterized in that, The cultivation method includes the following steps: (1) Controlling the light source environment: During the growth of red sea bass fry, use a blue light source with a wavelength range of 420-450nm to irradiate for 60-75 days; (2) Aquatic environment modification: Lay a layer of artificial material on the bottom of the water, introduce water with a salinity of 30-35‰ as aquaculture water, and use oxygen pumps to aerate all day long; (3) Alternate feeding of feed A and feed B: Alternate feeding until the juvenile stage; feed A is prepared by adding ox ear fungus to the basic feed, and feed B is prepared by adding loperamide to the basic feed; The artificial material, by weight, includes 12-20 parts of oyster shell powder-kaolin modified material, 10-15 parts of sodium silicate, 5-8 parts of superphosphate, and 4-10 parts of Clostridium butyricum metabolites. Step (2) The thickness of the artificial material to be laid is 3-6 cm; The preparation method of the oyster shell powder-kaolin modified material is as follows: by weight, 2-6 parts of oyster shell powder and 5-10 parts of kaolin are added to 10-20 parts of anhydrous ethanol, and after standing for 10-15 minutes, 1-3 parts of hexadecyltrimethoxysilane are added. The mixture is heated to 75-82°C while stirring, followed by ultrasonic vibration for 10-20 minutes. After filtration, washing with water, and drying, the modified material is obtained. The preparation method of the butyric acid clostridium metabolites is as follows: After activation, butyric acid clostridium is inoculated into a clostridium-enriched culture medium and anaerobic static culture is carried out at a temperature of 30-35℃ for 12-16 hours to obtain bacterial suspension; 1% of the bacterial suspension is inoculated into LB liquid culture medium and cultured at a temperature of 28-32℃ and a rotation speed of 220-260 rpm for 5-8 hours. After centrifugation, the supernatant is collected to obtain the butyric acid clostridium metabolites. The preparation method of the artificial material in step (2) is as follows: oyster shell powder-kaolin modified material, sodium silicate and superphosphate are mixed to obtain a mixture; 2 to 5 times the volume of anhydrous ethanol is added to the mixture, and the mixture is subjected to the first ultrasonic vibration and ball milling. Then, Clostridium butyricum metabolites are added and subjected to the second ultrasonic vibration to obtain the artificial material. The first ultrasonic oscillation frequency is 20-25 kHz, and the duration is 12-20 min; the second ultrasonic oscillation frequency is 30-40 kHz, and the duration is 5-10 min. In step (3), the amount of ox-ear fungus and loperamide added to feed A and feed B is 5-9 wt%, respectively, and the feeding frequency is 3 times / day, with a daily feeding amount of 3-6% of the fish fry's body weight.

2. The method for cultivating red snapper fry with high survival rate according to claim 1, characterized in that, The blue light source is placed 80-100cm above the aquaculture water.

3. The method for cultivating red snapper fry with high survival rate according to claim 1, characterized in that, The light intensity of the blue light source in step (1) is 100-150 lux, and the light period is 17L:7D.

4. The method for cultivating red snapper fry with high survival rate according to claim 1, characterized in that, The artificial material described in step (2) comprises, by weight, 15 parts oyster shell powder-kaolin modified material, 12 parts sodium silicate, 6 parts superphosphate, and 5 parts Clostridium butyricum metabolites.

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