Method for researching age and migration life history of coilia by eardust
A life history and otolith technology, applied in the field of fish ecology, can solve the problems of difficulty in marking the recapture operation, and no life history of dagger migratory, and achieve high efficiency.
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[0041] Below in conjunction with accompanying drawing and embodiment each step of the present invention is described in detail:
[0042] 1. Step ①
[0043] Open the brain of the anchovy, take out the otolith from the balloon, wash it with clean water, then wash it with 75% alcohol, and dry it; the purpose of alcohol cleaning is to remove the grease stuck on the otolith, so that it can be observed after grinding Effect.
[0044] 2. Step ②
[0045] Take the left sagittal otolith as the material for age identification, first use dissecting scissors to cut off the basal and wing leaves of the front end of the otolith, and cut out a plane that is completely perpendicular to the long axis of the otolith; choose a hot melt adhesive with higher hardness, Make a cube of about 1 cm in length, width and height, place it on a glass slide, heat it with an alcohol lamp until it melts, fix the cut otolith on the glass slide with hot melt adhesive, and ensure the length of the otolith The ...
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