Method for researching age and migration life history of coilia by eardust

A life history and otolith technology, applied in the field of fish ecology, can solve the problems of difficulty in marking the recapture operation, and no life history of dagger migratory, and achieve high efficiency.

Inactive Publication Date: 2009-10-07
INST OF AQUATIC LIFE ACAD SINICA
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  • Description
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AI Technical Summary

Problems solved by technology

The life history of fish migration is generally studied using the method of marking and releasing. Because the anchovy is small and dies when it comes out of

Method used

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  • Method for researching age and migration life history of coilia by eardust
  • Method for researching age and migration life history of coilia by eardust

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Experimental program
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Embodiment Construction

[0041] Below in conjunction with accompanying drawing and embodiment each step of the present invention is described in detail:

[0042] 1. Step ①

[0043] Open the brain of the anchovy, take out the otolith from the balloon, wash it with clean water, then wash it with 75% alcohol, and dry it; the purpose of alcohol cleaning is to remove the grease stuck on the otolith, so that it can be observed after grinding Effect.

[0044] 2. Step ②

[0045] Take the left sagittal otolith as the material for age identification, first use dissecting scissors to cut off the basal and wing leaves of the front end of the otolith, and cut out a plane that is completely perpendicular to the long axis of the otolith; choose a hot melt adhesive with higher hardness, Make a cube of about 1 cm in length, width and height, place it on a glass slide, heat it with an alcohol lamp until it melts, fix the cut otolith on the glass slide with hot melt adhesive, and ensure the length of the otolith The ...

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Abstract

The present invention discloses a method for researching age and migration life history of coilia by eardust which belongs to the ichthyological ecology field. The method selects the eardust as researching material, processes grinding to an intersecting surface of the eardust, observes a micro-structure of the eardust for researching age, growth and life history of the coilia. Compared with traditional method, the method provides a new method for researching fish type annual zone, fills blank of unclear bright band and dark lane on the eardust to research fish type age; the eardust grinding has high efficiency, each eardust can be ground only five minutes, and the result is more reliable than other hard bone tissue analyzing; a nail enamel has transparent function to the eardust in embedding and enveloping simultaneity which is more safety and has better transparent effect than using dimethylbenzene. The method is suitable for researching relative age and migration life history of all hard bone fish type with eardust back and belly morphology curve.

Description

technical field [0001] The invention belongs to the field of fish ecology, and in particular relates to a method for studying the age and migratory life history of anchovy through otoliths. Background technique [0002] The research object Coilia ectenes Jordan et Seale belongs to Clupeiformes and Engraulidae. It is an important economic fish in the middle and lower reaches of the Yangtze River and its affiliated water bodies. It usually lives in the Yangtze River Estuary and near the bottom of the shallow sea. During the breeding season, sexually mature individuals cluster up the river. Breeding began in Chongming in May, and as time progressed upwards, the breeding colony migrating to Anqing began to spawn in August. Historically, the Yangtze River is extremely rich in migratory anchovy resources, with a maximum annual output of 3,945 tons. The fishing season begins in February every year and reaches its peak in mid-March. The flood season is about 20 days, and the migrat...

Claims

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Application Information

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IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 何文平黎雨轩谢松光
Owner INST OF AQUATIC LIFE ACAD SINICA
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