Method for drawing blood from coilia ectenes under anaesthesia

A technology of anchovy and syringe, applied in the field of anesthesia and blood drawing of anchovy, can solve the problems of low efficiency, slow work progress, and easy death, and achieve the effect of low cost, large amount of blood drawn, and easy-to-learn method
CN101707983AInactive Publication Date: 2010-05-19FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI +1
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
Publication Date
2010-05-19
Estimated Expiration
Not applicable · inactive patent
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Abstract

The invention relates to a method for drawing blood from coilia ectenes under anaesthesia, and the operation steps are as follows: firstly adding mixed water of pond water and deep well water into a container, regulating the water temperature to 10-20 DEG C, and adding edible salt and tricaine sulfonate into the mixed water for preparing anaesthetic solution, wherein the salt degree is 5-7 per mill and the percentage concentration of the tricaine sulfonate is 0.01%-0.03%. The coilia ectenes is placed in the anaesthetic solution, and the anaesthetic time is 2-4 minutes. A syringe is used for drawing the blood, the part is located at the bottom of the spine above the anus, the time for drawing the blood is 1-1.5 minutes, and the coilia ectenes after drawing the blood is transferred into a temporary cultivation pond for regaining consciousness. The method can successfully carry out anaesthesia and blood drawing on the coilia ectenes against the physiological characteristics of the coilia ectenes, the method is simple, the cost is low and the volume of drawing the blood is large.
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Description

Technical field

[0001] The invention belongs to the technical field of fish breeding. Specifically, it relates to a method for drawing blood under anesthesia with coilia. Background technique

[0002] Coilia nasus (Coilia nasus) is one of the valuable migratory fishes that enjoys the reputation of "Three Fresh Products of the Yangtze River". Research on the germplasm preservation and artificial propagation technology of Coilia nasus has always been the focus and hotspot of domestic and foreign experts.

[0003] At present, with the rapid development of modern molecular biology technology and biochemical technology, various high-tech technologies and instruments such as DNA and RNA have also been continuously introduced into various research fields of Coilia. However, the efficiency of extracting DNA and RNA from the muscles and organs of Coilia spp. currently used is low, and many tasks that require blood to be measured are progressing slowly. Coilia vulgaris is extremely delicat...

Claims

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