53 results about "Order copepoda" patented technology

Larviculture is performed using Parvocalanus sp as a feed for fish larvae. A system is described using tanks for growing Parvocalanus sp nauplii with a microalgae feed and transferring the grown Parvocalanus sp nauplii to tanks containing the fish larvae, where the functions of the tanks is interchanged. The Parvocalanus sp feed provide for higher numbers of larger juvenile fish and the rearing of larvae heretofore not reared in culture.

The invention discloses a benthic diatom culture method for growing seedlings and an abalone fry culture method, which relate to an abalone culture technology. The invention is characterized by comprising the following steps: (1) starting culturing algaes 45 days before pulling seedlings, keeping the culture water temperature at 14 DEG C and the illuminance between 20001 and 30001x, controlling the light between 10001 and 15001x at the last culture after screening and eliminating algae culture for one and half a month, and laying ovum after 7 days; (2) adding nutrient salt at primary culture,wherein the adding ratio of N:P:Si:Fe is 5:1:1:1(ppm); (3) laying ovums about 5 millions for each pool and carrying out micro inflation; (4) changing water regularly after the ovums are laid and changing water twice a measuring range which is 24 hours; (5) carrying out running water culture after the ovums swim for 5 to 6 days and are adhered; (6) controlling the illuminance within 10001x after larvas metamorphose; and (7) dumping the pool after treating copepods at about 30 days of the culture period, adjusting the illuminance between 15001 and 20001x and peeling off baby abalones after about45 days. The methods have the advantages of improving the yield and preventing and treating a plate stripping disease.

The invention relates to a composite nutrition enhancer and a preparation method thereof, relating to a feed additive. The invention provides the composite nutrition enhancer, which not only contains large amount of DHA, ARA and natural yeast astaxanthin, but also contains multiple Omega 3 unsaturated fatty acids, multiple amino acids and trace elements. The composite nutrition enhancer is formedby composition of fission chytrid microalgae and phaffia rhodozyma with the weight ratio of 50 percent to 80 percent and 20 percent to 50 percent. All the components are weighed and mixed according to matching ratio after being crushed, mixed evenly, powder-sieved till the particle size is 8-25mum, inspected, weighed and packaged. The invention is especially applicable to feeding of live eels fryand shrimp postlarvae, artificial seawater seedling enhanced artemia salina sinnaeus, rotifer and cladocera (micrura), copepods and the like and various egg-laying poultries.

A method to killing copepod in Rotifer culture relates to bait applicator culture aimed at solving the problem of killing copepod in Rotifer culture. The technology program of the invention includes: equipping inflatable stone in the pool, injecting seawater and seawater Chlorella, it is characterized by equipping inflatable stone in the pool by one/ m2, injecting seawater and seawater Chlorella by3:1 to 4:1, the salinity of mixed seawater is 15-20%., the rotifer asylum density is 1000-1500/ml, the water temperature is 25-30degreeC, pouring the anthon solution into the pool by the density of 1.2g/m3, collecting the rotifer using the 250 mesh sieve silk bags after 24 hours and maintaining inflatable in the rotifer process. The invention applies to the bait applicator culture of fish, shrimp, crab seed.

The invention discloses an ecological Japanese sea horse breeding method. The ecological Japanese sea horse breeding method comprises the steps of building an ecological sea horse breeding pond one week before breeding, cultivating microalgae in the built ecological breeding pond, and measuring and controlling water quality to reach newborn sea horse breeding indexes before newborn sea horses are bred. When the newborn sea horses is younger than 15 days, the released density is 1000-1200 tail/m<3>, and baits are composed of rotifer, copepods larvae, chlorella, photosynthetic bacteria, tetraselmis and fairy shrimps which are just incubated. During 15-30 days, the released density is 600-1000 tail/m<3>, baits are composed of fairy shrimps, copepods larvae and shrimp seeds, and the baits are fed into the breeding pond four times per day. A sea horse juvenile fish breeding stage comprises the step of enabling the water quality to reach juvenile fish breeding indexes before breeding. During 30-40 days, the released density is 600-800 tail/m<3>, and the baits are fed into the breeding pond three times per day. A sea horse adult fish breeding stage comprises the step of enabling the water quality to reach sea horse adult fish breeding indexes. The released density of sea horse adult fish is 200-400 tail/m<3>, baits are fresh and alive shrimps, and the baits are fed into the breeding pond three times per day. The ecological Japanese sea horse breeding method has the advantages of reducing production cost.

The invention discloses a cultivation method for green crab seedlings in a pond. The cultivation method consists of seedling cultivating facilities and a seedling cultivating process and is characterized by comprising the following steps of: preparing a mud or sediment bottom seedling cultivating pond; arranging miropores for inflating and an aerator in the seedling cultivating pond; carrying out pond cleaning and sterilization on a water reservoir and the seedling cultivating pond before the seedling is cultivated; filtering inflowing water by using a bolting-cloth mesh bag with 120 meshes; directly incubating green crab larva in the seedling cultivating pond; starting all aerating facilities from the second day after the green crab larva are incubated; feeding the green crab larva with the following baits: feeding with wheel animalcule at the earlier stages of Z1 to Z3, preventing copepods from entering into a seedling cultivating water body at the stages of Z1 to Z2, feeding with the wheel animalcule and supplementing the feeding of the copepods with small specifications at the later stage of Z3, feeding with the copepods with small specifications at the stage of Z4 and feeding with the copepods at the stage of Z5; and gradually adding new water from the stage of Z3, wherein the water level at the initial stage of seedling cultivation is 70-80cm and the water quality indexes during seedling cultivation are that dissolved oxygen is not smaller than 5mg/L, the contents of ammonia and nitrogen are not greater than 0.4mg/L, the pH value is 7.8-8.5 and the salinity is 28-34.

The invention provides a method for culturing and transplanting Scylla fries stage by stage, which relates to a Scylla culture method. The method comprises the following steps of: culturing zoeae in a culture pond for culturing for 12 to 18 days, and transferring the zoeae into a megalop culture pond with the area of 100 to 300 square meters for further culture; culturing live foods such as algae, fairy shrimps, copepods and the like in the megalop culture pond; transferring the fries at the last day of the fifth stage of the zoeae or at the first day of megalop, wherein the culture density of the megalop is controlled to be 1,000 to 2,000 fries per cubic meter; or culturing the zoeae at the fifth stage for 4 to 8 days, wherein the culture density is 2,000 to 4,000 fries per cubic meter; the water temperature of the megalop culture pond is between 24 and 32 DEG C; and the salinity is 1.5 to 2.6 percent; and transferring the megalop into a young Scylla culture pond, wherein the area ofthe young Scylla culture pond is 0.5 to 3 mu; the water temperature is between 26 and 33 DEG C; and the salinity is 1.0 to 2.0 percent.

The invention provides a breeding method for amphiprion ocellarises. The breeding method comprises the following three steps: a. breeding parent fishes; b. incubating oosperms; c. breeding young juvenile fish. Fry is bred for 60 d after being incubated, the overall length of the juvenile fish is up to 2 cm to 3 cm, and the breeding process is ended up; during the breeding process, the minced-meat-shaped fresh bait made of rotifer intensified by seawater chlorella for 24 h, artemia and copepods intensified by DHA and the chlorella for 12 h, and marine small animals of which phospholipid content is more than 25% in succession and compound feed are fed as the bait in an individual manner or a superimposed manner; furthermore, water is changed and the amphiprion ocellarises are divided and put in fish tanks in proper time. The breeding method for the amphiprion ocellaris, provided by the invention, provides a way which can be operated in an industrialized and large-scale manner; compared with the prior art, as the breeding method provided by the invention is of popularity to a higher degree, a foundation is provided for large-scale breeding of the amphiprion ocellaris.

The invention discloses a technology for cultivating takifugu flavidus summer fingerlings in a cement pond of a simple plastic greenhouse. The cement pond is adopted, and an arch-shaped ring top is formed above the cement pond. The technology is characterized in that: a dimmable shading film covers on the arch-shaped ring top; the air density in the cement pond is 1-1.5 pieces per square meter; the stocking sizes are 10-12 millimeters in full lengths per fingerling; the stocking density is 1,000-1,500 fingerlings per square meter; the water level is 70 centimeters during stocking; 20 centimeters of fresh water is injected every day from the second day, the cement pond is full of water four days later, and 50 percent of water is changed every other day from the fifth day; after 10-15 days of cultivation, bait scraps and fish excrement are cleared; the water quality indexes are that: the salinity of sea water is 0.8-1.2 percent, the water temperature is 25-28 DEG C, the dissolved oxygen is over 5 mg/L, and the pH is 7.5-8.5; medium-sized cladocerans and copepods are fed at an earlier stage, and large frozen fairy shrimps are fed at a middle stage; and after the full lengths of fingerlings are up to 30 millimeters, a compound feed is fed instead of the frozen fairy shrimps, and an eel feed is fed completely after 3-4 days of transition.

The invention relates to a feeding method of controllable bait for fries of Takifugu flavidus. The feeding method is characterized by comprising the following steps of: taking egg yolk as the bait during the initial stage when the fries are aged 2-10 days, and uniformly splashing and feeding in a full pool six times per day; taking Artemia larvae which are justly hatched as the bait when the fries are aged 7-10 days, and supplementing twice per day, wherein the feeding density is 2-3 larvae/ml; taking the Artemia larvae as the bait when the fries are aged 11-19 days, and supplementing twice per day, wherein the feeding density is 3-5 larvae/ml; taking frozen copepods as the bait when the fries are aged 14-19 days, uniformly splashing and feeding in the full pool, and supplementing six times per day, wherein the feeding density is 4-6 copepods/ml; taking the frozen copepods as the bait when the fries are aged 20-25 days, uniformly splashing and feeding in the full pool, and supplementing six times per day, wherein the feeding density is 4-6 copepods/ml; taking frozen large Artemia as the bait when the fries are aged 25-35 days, melting the bait at normal temperature, feeding to a bait table, and supplementing three times per day; and feeding compound feed when the fries are aged 30-35 days, placing 4-6 blocks of the compound feed on each bait table, and feeding twice per day, wherein the compound feed is kneaded into pastes.

The invention discloses a method for mixed cultivation of sea horses and hemifusus ternatanus.Hemifusus ternatanus and sea horses are cultivated inside a culture pond to construct a fish and shellfish circulation ecological cultivation system, suitable culture objects can adapt to all ecological niches and trophic levels in the culture pond, and bait waste and water pollution caused by residual bait are avoided.The sea horses and the hemifusus ternatanus occupy different ecological niches in the cultivation environment respectively, various kinds of natural bait in water and artificial feed put into the water can be sufficiently utilized, the total conversion rate of objects and energy in the culture pond is increased, the water quality is effectively stabilized, and entrance of etiology is controlled.The sea horse bait mainly includes copepods, artemia and small shrimps; the hemifusus ternatanus is demersal sarcophagy, and no additional feeding is needed in the cultivation process, so that the hemifusus ternatanus eat residual bait generated in the cultivation process of the sea horses, and the role of a scavenger is brought into play.Accordingly, the purposes that the bait utilization rate is improved, water pollution caused by residual bait is avoided, diseases are prevented and treated, and the production cost is saved are achieved.

A method for sea anemone culture in a pond is characterized in that a net cage suspended under the water surface of the pond is used for artificial culture. The net cage is shaped like a cuboid with dimensions of 2*1*1m, the upper face of the net cage is opened, the bottom face of the net cage and the surrounding side faces are fenced by net plates, and the net size is eight meshes; the multiple net cages are disposed and arranged in a line along the length direction of the net cages, the upper edge of each net cage is connected by two parallel main ropes, multiple supporting rods are fixed at an interval between the two main ropes, and a floating ball is connected at the joint between each supporting rod and the main rope; auxiliary ropes are connected to two end heads of the main rope, and the end head of each auxiliary rope is connected to a rope anchor located at the bottom of the pond; and an attaching baseplate is disposed in the net cage, the attaching baseplate comprises a bottom plate, a corrugated plate is fixed on the bottom plate, and through holes are arranged in the corrugated plate. Materials could be obtained easily; the method is simple; operations are easy; management is easy; sea anemones could be bred separately or bred together with trepang to eat trepang enemies such as copepod and gammarid; space in a pond water area is fully used; the survival rate is high; and the growth speed is high.

The invention discloses a method for preventing and controlling copepods in stichopus japonicus seedling culture. The method is characterized by comprising the following steps: selecting jelly fish seedlings with the umbrella diameter of 3-4 cm; putting 4-5 jelly fish seedlings into each cubic meter of water in a pond with copepods in a stichopus japonicus seedling culture workshop; determining the total number of jelly fish seedlings put into each pond, wherein the jelly fish seedlings eat the copepods in the stichopus japonicus seedling culture pond, and can eat over 95% of the copepods in the stichopus japonicus seedling culture pond after 1-2 days without hurting the young stichopus japonicus; and finally fishing out the jelly fishes in the stichopus japonicus seedling culture pond and putting into other ponds with copepods to eat the copepods continuously, wherein feeding and water exchange management during the stichopus japonicus seedling culture are the same as normal management. By adopting the method disclosed by the invention, the jelly fish seedlings can be uniformly distributed in water to continuously eat copepods in water so that the copepods in the stichopus japonicus seedling culture pond are eaten without using medicine; and moreover, the method prevents the death of stichopus japonicus seedlings caused by the copepods in the stichopus japonicus production, improves the survival rate of stichopus japonicus and provides guarantee to the stable high yield of stichopus japonicus seedlings on one hand, and on the other hand, reduces the application of trichlorfon and miezaoling, improves the quality of stichopus japonicus seedlings and is environment-friendly while culturing the jelly fishes and reducing the production cost.

The invention discloses a method for extracting DNA from single eggs of copepods, relating to a DNA extraction technology. The single eggs have different egg diameters and are generated by different egg producing ways. The method can ensure the extraction efficiency of DNA, and the extracted DNA can be directly applied to downstream PCR experiments. The method comprises the following steps of placing the single eggs of copepods in a 1*TE buffer solution, adding protease K until the final concentration of the protease K reaches 2.5-5.5mg/mLto obtain a mixed extraction solution; carrying out a thermal incubation program on the mixed extraction solution obtained to obtain a crude extract of DNA of the single eggs; and depositing the crude extract of DNA of the single eggs with a nucleic acid coprecipitator to obtain DNA precipitate. The precipitate can be dissolved in sterilized water and stored in a refrigerator for later use.

The invention discloses a method for preventing and controlling copepods in a young sea cucumber breeding pond of tropical sea cucumbers. The method comprises the steps of after the young sea cucumbers are subjected to metamorphosis and attachment for two weeks and the length of the young sea cucumber is 0.5cm the young sea cucumber is 0.5cm long, putting amphiprioninae or chrysiptera cyaned or both into the breeding pond when copepods appear in the breeding pond, and culturing and managing the young sea cucumbers according to a conventional method. A large number of experiments show that the amphiprioninae and chrysiptera cyaned can coexist with the young tropical sea cucumbers without damaging the same and can control the copepods, so that the survival rate of the young sea cucumbers is high. Compared with the prior art, the method can reduce the use of medicines, is beneficial to protecting environments and improving the quality of sea cucumbers, and meanwhile, reduces workload in a young sea cucumber culturing period, and avoids mechanical losses of the young sea cucumbers in an operating process, thus being beneficial to the rapid and healthy growth of the young sea cucumbers.

The invention relates to an ecological cultivating method for initial feed of sebastiscus marmoratus larva. The method is characterized in that a cement or film side slope is arranged in a feed cultivating pool; the non-leaking muddy or sandy sediment is arranged as the bottom sediment; the water depth is 1-2m; independent water feeding/draining facility and aerator are arranged. The method comprises the following steps: sterilizing the feed cultivating pool with water containing 20ppm bleaching powder in the period from each December to the January in next year; after 2 days, dryly disinfecting the pool with 150kg/mu quick lime; exposing for one week and then feeding water; covering a 60-mesh silk gauze at a water inlet, wherein the water resource is natural river water and the salinity is 1.2-2.0; collecting copepods from the other pond and inoculating into the feed cultivating pool, after water is fed into the feed cultivating pool for one week and water in the pool becomes yellow green; after inoculating the copepods, stocking palaemon carinicauda and 2-year old coilia ectenes and feeding prawn mixed feed for one time per day; collecting the small zooplankters including copepods nauplius, small anhistozoa, rotifer, and the like, as the initial feed of sebastiscus marmoratus larva, when the water temperature is 15 DEG C or above.

The invention discloses an automatic cleaning device for copepod biological bait and belongs to the technical field of bait cleaning devices. The automatic cleaning device comprises a bolting silk baghanging inside a box body; a jolting board is arranged under the bolting silk bag; a flipper is connected to the lower part of the jolting board through a spring; the flipper is connected to the boxbody through a support; a motor is installed at a lower side of one end of the flipper; a flushing plate is installed over the bolting silk bag. The cleaning device of the invention can automaticallyand efficiently clean the copepods, and the biological integrity after cleaning is high, saving manpower and material resources.

The invention discloses a method for measuring activity of maltase in copepods. The method comprises the following steps of: grinding and centrifuging 5 to 15 copepods in a buffer solution, reacting with a maltose substrate in a water bath to obtain a water bath product, mixing the water bath product, Tris-HCl, MgCl2, dithiothreitol, hexokinase, nicotinamide adenine dinucleotide phosphate (NADP)<+>, adenosine triphosphate (ATP) and glucose 6-phosphate dehydrogenase, performing enzymatic reaction, and calculating the activity of the maltase in each copepod according to a formula by using absorbance of reductive coenzyme II in the enzymatic reaction product and the number of the copepods as parameters. According to the method, the activity of digestive enzyme can be measured only by a few to more than ten copepods, and the absorbance of the reductive coenzyme II can be obtained by the water bath reaction and the enzymatic reaction of the maltase in the copepods; and the method is easy and convenient to operate, short in reaction time and less in errors, so the method has the advantages of easiness and convenience in operation, low sample consumption, short test time and high test efficiency.

The invention relates to a method for manually breeding Charybdis feriatus seedlings in an earth pond and belongs to the technical field of seedling culture for aquaculture. The method comprises the following steps that incubated parent crabs subjected to intensive culture in a concrete pond are placed in an incubation basket which is prepared in the earth pond in advance, rotifers are fed at the same day of incubation, the water color of the earth pond is observed every day and the transparency of the earth pond is kept about 35 cm; after being developed to the third stage of zoea, copepods are fed to the earth pond, and the density of the copepods is kept at 5 per milliliter; during megalops period, fish and meat are fed to the earth pond according to 200% of the estimated larva weight until the larvae are developed to young cabs. During the seedling breeding period, the requirements on the water quality are that the salinity is reliably stable and is kept at 2-3%, the temperature is 26-29 DEG C, the pH is 7.8-8.6, the dissolved oxygen is more than 4 mg/L and the transparency is kept at 30-50 cm. According to the method, a technology for manually breeding the Charybdis feriatus seedlings in the earth pond is implemented, and the method is simple, quick and low in production cost, and provides a feasible way for scale production of the Charybdis feriatus.

This invention relates to a biological oil composition, preferably obtained from a copepod, most preferably the copepod Calanus finmarchicus and the use thereof to prevent or treat formation of atherosclerotic plaques and hence development of coronary heart disease. The composition comprises the same marine n-3 polyunsaturated fatty acids (PUFAs) generally regarded as being responsible for the anti-atherosclerotic effect of marine oils, namely EPA (C20:5n-3 eicosapentaenoic acid) and DHA (C22:6n-3 docosahexaenoic acid). However, quite unexpectedly, it has been found that the oil composition of the present invention has a remarkably higher ability to prevent formation of atherosclerotic plaques than what can be attributed to EPA and DHA alone, and moreover, unlike EPA and DHA alone it has a notable blood cholesterol lowering effect.

The invention relates to the technical field of aquaculture, in particular to a method for ecologically breeding coilia nasus schlegel fries in a takifugu obscures culture pond, which comprises eightsteps of pond preparation, stocking of coilia nasus schlegel parents, inoculation of cladocerans and copepods, stocking of takifugu obscures, bait feeding, stocking of brooding freshwater shrimps, daily management, and net pulling for catching. The invention fully utilizes the water space of a takifugu obscurus parent culture pond, increases yield of the adult coilia nasus schlegel and large-sizedfries under the conditions of not increasing the input and not reducing the takifugu obscures parent culture density, improves the water utilization rate of the pond, improves the economic benefit, makes the water quality of the pond more stable, improves the feed utilization rate, and reduces the takifugu obscures culture cost; the method of the invention is easy to operate and can be easily accepted and promoted.