Improved culture medium for short-term proliferation of cultured meat seed cells

A technology of proliferation medium and seed cells, which is applied in the field of improved medium for cultivating meat seed cells in vitro for short-term proliferation, can solve problems such as environmental pollution, difficulty in meeting human meat needs, animal welfare, etc., and achieve faster proliferation and better Differentiation potential, effect of enhancing differentiation ability

Active Publication Date: 2021-04-09
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] With economic development and population expansion, traditional meat production methods are increasingly difficult to meet human m...

Method used

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  • Improved culture medium for short-term proliferation of cultured meat seed cells
  • Improved culture medium for short-term proliferation of cultured meat seed cells
  • Improved culture medium for short-term proliferation of cultured meat seed cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Example 1 Porcine muscle stem cells proliferative ability detection in vitro short-term culture:

[0054] 1) Short-term culture of muscle stem cells: this embodiment is divided into 4 groups, which are respectively the conventional proliferation medium control group and the experimental group of the improved proliferation medium group with a water-soluble vitamin E concentration of 50 μmol / L, and a water-soluble vitamin E concentration of 100 μmol / L. The experimental group of the modified proliferation medium group of L, the experimental group of the modified proliferation medium group with the concentration of water-soluble vitamin E at 200 μmol / L. Porcine muscle stem cells were divided into 1.5×10 5 / dish was inoculated into 10cm culture dishes of conventional proliferation medium and improved proliferation medium, and the medium was changed for two days, digested with 0.25% trypsin for three days, and counted with a hemocytometer.

[0055] 2) The results show that t...

Embodiment 2

[0056] Example 2 Detection of Muscle Stem Cell Differentiation Potential

[0057] Get the conventional proliferation medium control group in Example 1, the improved proliferation medium group experimental group whose water-soluble vitamin E concentration is 50 μmol / L, the water-soluble vitamin E concentration is 100 μmol / L, and the water-soluble vitamin E concentration is 200 μmol / L. The cells cultured in the proliferation medium group and the experimental group were extracted from the RNA, and the gene expression of the stemness gene "Pax7" that characterizes the differentiation potential of the cells cultured in the improved proliferation medium and the conventional proliferation medium was detected by q-PCR technology.

[0058] The results showed that the short-term culture of the muscle stem cells in the improved proliferation medium added with water-soluble vitamin E could maintain its differentiation potential. When the concentration of water-soluble vitamin E was 50 μmol...

Embodiment 3

[0059] Example 3 Detection of Muscle Stem Cell Differentiation Level

[0060] Take the muscle stem cells obtained from the modified proliferation medium and conventional proliferation medium in Example 1 where the water-soluble vitamin E is 100 μmol / L, and then induce differentiation: the process of muscle stem cell differentiation in vitro includes two stages, the first stage is muscle stem cells In the proliferation stage of stem cells, the cells are propagated in a differentiation culture dish containing improved proliferation medium and conventional proliferation medium added with water-soluble vitamin E, and the proliferation reaches the pre-differentiation stage after 5 days.

[0061] The cells in the pre-differentiation stage cultured with the improved proliferation medium and conventional proliferation medium supplemented with water-soluble vitamin E were induced to differentiate, and the medium was changed every two days, that is, half of the differentiation medium was...

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Abstract

The invention provides an improved culture medium for short-term proliferation of cultured meat seed cells, which can accelerate the proliferation speed of the current most potential seed cells-muscle stem cells of cultured meat in short-term culture in vitro. The improved culture medium is a cell proliferation culture medium added with water-soluble vitamin E, the proliferation speed of the cells cultured by the improved proliferation culture medium in short-term culture in vitro is significantly improved, and the number of the cells is 1.2 times of that of the cells cultured by a normal culture medium; and the cells cultured by the improved proliferation culture medium added with the water-soluble vitamin E are induced and differentiated in a differentiation culture medium, so that the differentiation ability of the cells can be significantly improved, and more muscle proteins can be produced. Therefore, the improved proliferation culture medium can help to obtain a large number of cultured meat seed cells in short term in vitro, and plays a very beneficial role in the production of the cultured meat.

Description

technical field [0001] The invention belongs to the technical field of stem cells and animal cell cultured meat, and in particular relates to an improved medium for short-term proliferation of meat seed cells in vitro. Background technique [0002] With economic development and population expansion, traditional meat production methods are becoming more and more difficult to meet human meat needs. At the same time, traditional animal husbandry is facing many challenges, such as environmental pollution and animal welfare issues. Therefore, we need efficient and environmentally friendly new meat production methods. The birth of cultured meat technology can meet human demand for meat in the future. Cell-cultured meat refers to the use of technologies such as cell culture engineering and tissue engineering to cultivate animal muscle tissue in vitro as food materials. It does not rely on animal breeding and directly produces meat through cell factories. According to estimates, com...

Claims

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Application Information

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IPC IPC(8): C12N5/077C12N5/074
CPCC12N5/0659C12N5/0658C12N2500/38C12N2501/115
Inventor 周光宏唐长波胡荣蓉丁世杰朱浩哲
Owner NANJING AGRICULTURAL UNIVERSITY
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