40 results about "Muscle protein" patented technology

The invention relates to a Kluyveromyces marxianus strain with high protein yield and application of the Kluyveromyces marxianus strain, which is characterized in that the Kluyveromyces marxianus strain is firstly cultured on a slant, then is transferred into a seed culture medium for seed preparation, and then is inoculated into a fermentation tank, and yeast protein is obtained by adopting index feeding; the bacterial strain is a dominant mutant strain Kluyveromyces marxianus YWX-1, the protein content in a dry thallus reaches 55% or above and is increased by 26% or above compared with that of an original bacterial strain, the bacterial strain can be used for production of single-cell protein, the essential amino acid index (EAAI) value in the protein reaches 1.2, and the bacterial strain is rich in arginine, threonine and lysine and can be applied to production of single-cell protein. These amino acids play a key role in immune regulation, intestinal mucosal barrier function and muscle protein synthesis of young animals. The YWX-1 kluyveromyces marxianus strain has a wide application prospect in the production of neoplasm protein.

The application discloses a meat protein emulsion with low-sodium and low-oil phase characteristics for 3D printing and a preparation method and application thereof. The application utilizes anionic polysaccharide xanthan gum and L-arginine to synergistically stabilize the low-sodium and low-oil phase meat protein emulsion, and improves the 3D printing performance thereof. The xanthan gum and L-arginine enhance the stability and rheological properties of the emulsion by inducing the unfolding of meat protein structure and reducing the oil-water interfacial tension. The xanthan gum can also prevent the aggregation of droplets by constructing a weak gel network in the water phase and providing negative charges. Therefore, the meat protein emulsion stabilized by the synergistic action of the xanthan gum and L-arginine overcomes the problems of poor emulsion stability and inability to print caused by partial replacement of sodium salt and low oil phase, and endows the meat protein emulsion with good viscoelasticity, thixotropic recovery performance and self-supporting property, thereby realizing high-fidelity printing. The application recommends the dual use of the xanthan gum and L-arginine, which can not only reduce the intake of sodium and oil to ensure health, but also help produce high-quality or personalized muscle protein food.

The invention belongs to the field of fish breeding, and discloses a construction method and primers of a fast-growing red crucian carp homozygous strain, and the construction method comprises the following steps: preparing gRNA of a red crucian carp acvr2b gene, microinjecting a mixture of the gRNA and Cas9 protein into a one-cell stage fertilized egg of the red crucian carp, and screening to obtain an F0-generation gene knockout mutant; carrying out selfing on the F0-generation gene knockout mutant to obtain an F1-generation mutant, and screening to obtain a homozygous mutant; and selfing and propagating the homozygous mutant to obtain the product. The acvr2b homozygous knockout strain is successfully established in the red crucian carp through a CRISPR / Cas9 gene editing technology for the first time, the body weight of the obtained acvr2b mutant red crucian carp at the age of 12 months is increased by 30.68% compared with that of a wild type, the muscle protein content is increased by 13.81%, the intestinal digestion and absorption capacity is obviously improved, high-quality germplasm resources are provided for fish genetic breeding, and the acvr2b mutant red crucian carp has a good application prospect.

The invention discloses application of caffeic acid phenethyl ester to improvement of negative effects of high-carbohydrate feed on fish growth and metabolism, and belongs to the technical field of aquaculture. The caffeic acid phenethyl ester is added into a high-carbohydrate feed formula, so that the metabolic capability of adipose tissues, livers and muscles of the grass carp and the weever on glucose can be remarkably enhanced, ectopic deposition of lipid in the livers and the muscles is relieved, fatty liver diseases are improved, muscle protein deposition is promoted, and the immunity of the grass carp and the weever is improved. The inhibiting effect of the high-carbohydrate feed on the growth performance of the grass carp and the weever is reversed. The invention further provides formulas of the high-carbohydrate feed containing the caffeic acid phenethyl ester for the grass carp and the weever respectively, and data support is provided for research on improvement of the utilization capacity of the grass carp and the weever on the high-carbohydrate feed.

Administration of certain chromium complexes in combination with a starch provide unexpected benefits regarding increasing amino acid absorption, protein synthesis, exercise tolerance, lean muscle mass, skeletal muscle hypertrophy, muscle power, muscle endurance, muscle strength, FSR, and decreasing delayed onset muscle soreness, muscle protein breakdown, and fat mass.

The invention relates to the field of food processing, and discloses a preparation method of a flavored duck, and a processing technology of the flavored duck mainly comprises the following steps: unfreezing; performing shaping; performing pickling; hanging and airing; charcoal roasting; baking with steam; and vacuum packaging. The curing effect of a Maillard reaction precursor substance in the duck meat product is enhanced through the pickling-air drying-charcoal roasting process, the steam roasting is combined, muscle protein is modified through the Maillard reaction, dicarbonyl compounds and lipid in an intermediate product react with degradation products of thiamine, a compound with meat fragrance is generated, and the meat flavor is improved. The flavor adsorption capacity of the duck meat product protein is improved. Besides, absorption of the pickling liquid is promoted through a rolling massage technology, the water-retaining property and the texture of the duck meat product are improved, and sodium erythorbate, tea polyphenols and a rosemary extract are added, so that the problem of color fading in the processing process and the later period of the flavored duck is effectively solved. The preparation method of the flavored duck provided by the invention is simple in processing flow and convenient to operate, and can be widely applied to the processing process of the flavored duck.

The invention provides a China-hemp peptide nutrition reduced protein bar and a preparation method thereof. The China-hemp peptide nutrition reduced protein bar comprises 10-20 parts of China-hemp peptide powder, 10-20 parts of resistant dextrin, 5-10 parts of chia seeds, 5-8 parts of psyllium husk powder, 6-10 parts of China-hemp seeds, 4-7 parts of pumpkin seed kernels, 1-2 parts of stevioside and 1-2 parts of China-hemp seed oil micro-capsule powder. The China-hemp peptide is applied to the fat-reducing protein bar, the proportion of branched-chain amino acid rich in the China-hemp peptide to essential amino acid is close to the metabolism requirement of a human body, the China-hemp peptide can directly participate in muscle protein synthesis, muscle catabolism is inhibited, the basal metabolic rate is effectively maintained in the fat-reducing period, and muscle loss is prevented. The resistant dextrin and the psyllium husk powder can prolong gastric emptying time and induce satiety hormone secretion, high-quality unsaturated fatty acid provided by the hemp seeds and the pumpkin seed kernels can regulate expression of lipid metabolism related genes and promote fat oxygenolysis, and conversion from carbohydrates to fat is reduced due to the rapid energy supply characteristic of the medium-chain triglyceride.

The invention discloses a preparation method of muscle protein soft meal food suitable for being swallowed by old people, and belongs to the technical field of food processing. According to the invention, the myofibrillar protein is subjected to charge modification by a PG enzymolysis method and is mixed with the vegetable puree in different proportions, and the mixture is subjected to thermal induction to form a reversible gel system, so that IDDSI II-IV level is covered, and the requirements of patients with mild to severe dysphagia are met. The system viscosity is reduced, and the colloidal feeling caused by a traditional thickening agent is avoided; the high-fiber vegetable puree enhances cohesion, solves the problem that natural vegetable puree is easy to layer, and the modified myofibrillar protein and the vegetable puree are mixed, so that the problems that the vegetable puree is high in viscosity and not easy to swallow after being heated are solved.

The application belongs to the field of fish breeding, and discloses a construction method and primers of a homozygous line of fast-growing red crucian carp. acvr2b The construction method comprises the following steps: preparing gRNA of a red crucian carp gene, microinjecting a mixture of the gRNA and a Cas9 protein into a one-cell fertilized egg of the red crucian carp, screening to obtain F0 generation gene knockout mutants, self-crossing the F0 generation gene knockout mutants to obtain F1 generation mutants, screening to obtain homozygous mutants, and self-crossing the homozygous mutants to propagate. acvr2b The application successfully establishes a homozygous knockout line in the red crucian carp for the first time through a CRISPR / Cas9 gene editing technology. acvr2b The mutant red crucian carp obtained in the application has a body weight increased by 30.68% and a muscle protein content increased by 13.81% compared with a wild type at 12 months of age, and has a significantly improved intestinal digestion and absorption capacity, thereby providing high-quality germplasm resources for fish genetic breeding and having a good application prospect.

Proposed is a muscle strength-enhancing composition being capable of maintaining or strengthening muscles or preventing muscle-related diseases. More specifically, proposed is a food composition containing a composite extract as an active ingredient obtained by mixing Angelica keiskei and Morus alba L. at a weight ratio of 1:8 to 16 and extracting the resulting mixture with alcohol. The food composition promotes antioxidant activity (for example, DPPH scavenging activity and ABTS scavenging activity) and muscle function enhancement activity which suppresses muscle protein breakdown and promotes muscle building, whereby the food composition prevents muscle-related diseases by enabling muscles to be maintained or strengthened.

The present disclosure relates to highly purified (R)-3'-hydroxybutyl (R)-3'-hydroxybutyrate and biomedical uses thereof. In particular highly purified (R)-3'-hydroxybutyl (R)-3'-hydroxybutyrate, is provided for enhancing bodily function and treating various disease states. The methods described herein provide for production of a highly purified (R)-3'-hydroxybutyl (R)-3'-hydroxybutyrate via processes external to the human body and administering effective amounts of highly purified (R)-3'-hydroxybutyl (R)-3'-hydroxybutyrate to human subjects to achieve a range of therapeutic benefits, including but not limited to, one or more of: inhibiting inflammasome activation, reducing pro-inflammatory cytokines, improving insulin sensitivity, enhancing mitochondrial biogenesis, and promoting muscle protein synthesis. Additionally, highly purified (R)-3'-hydroxybutyl (R)-3'-hydroxybutyrate is administered in an amount and frequency conducive to one or more of: improving cognitive function, reducing oxidative stress, enhancing cardiovascular health, and supporting neuroprotection. Methods for manufacturing and purifying (R)-3'-hydroxybutyl (R)-3'-hydroxybutyrate compositions to ensure high specificity and efficacy in therapeutic applications are also provided.

This invention discloses a high-efficiency frying process for crispy, low-fat prepared chicken products. The invention relates to the field of chicken product processing technology. The process includes: raw meat processing, auxiliary ingredient preparation, low-temperature chopping, gradient vacuum intermittent tumbling, low-temperature settling, shaping, low-temperature pre-curing, two-stage differential temperature frying, gradient clean air cooling, ultra-low temperature quick-freezing, inspection, packaging, and low-temperature storage. This high-efficiency frying process for crispy, low-fat prepared chicken products effectively inhibits the temperature rise of the raw meat during processing by combining staged low-temperature controlled chopping with gradient vacuum intermittent tumbling. This maximizes the preservation of the natural characteristics of muscle proteins, promotes the full dissolution of myofibril proteins, and forms a dense, uniform, and highly stable gel network structure. This significantly improves the water retention and structural strength of the product, resulting in a delicate, juicy, and tender texture, while also laying a solid structural foundation for subsequent low-oil-absorption frying.

The invention belongs to the technical field of special diet food, and discloses muscle-building and qi-tonifying protein powder and a preparation method thereof, the muscle-building and qi-tonifying protein powder comprises the following components by weight: 75%-80% of a compound protein matrix, 10%-12% of a slow-release carbon-water system, 8%-10% of a medicinal and edible spleen-tonifying composition and 0.5%-2% of a function enhancer; wherein the slow-release carbon water system is composed of low-GI maltodextrin and oat powder, the medicinal and edible spleen-tonifying formula is composed of an astragalus extract, a Chinese yam extract and a white hyacinth bean extract, the compound protein matrix is composed of whey protein, casein and soybean protein isolate, and the function enhancer is composed of BCAA peptide and glutamine. According to the protein powder disclosed by the invention, through a'protein-carbohydrate-spleen-tonifying extract 'ternary synergistic mechanism, while the muscle protein synthesis rate is increased, the incidence rate of spleen deficiency type dyspepsia is remarkably reduced, and the incidence rate of upper respiratory tract infection is reduced.

To provide methods and compositions for stimulating muscle protein synthesis.SOLUTION: Provided is a method for stimulating muscle protein synthesis, the method comprising administering to a subject a composition comprising the following concentrations of amino acids in terms of w / w%: about 1 to 2% of histidine, about 9 to 11% of isoleucine, about 35 to 38% of leucine, about 14 to 17% of lysine, about 2 to 4% of methionine, about 5 to 7% of phenylalanine, about 8 to 9% of threonine, about 9 to 11% of valine, about 0.005 to 0.8% of tryptophan, and about 8 to 11% of arginine, and increasing the stimulation of muscle protein synthesis compared to a subject not administered the amino acid composition.SELECTED DRAWING: None

In some embodiments, the gene is integrated into the genome of the cyanobacteria. In some of these embodiments, the gene is integrated into an NS1 site of the cell. The sequence encoding an animal protein is optimized for expression in the cyanobacteria. In some embodiments, the animal protein is a muscle protein. In some of these embodiments, the muscle protein is a myosin subunit, myoglobin, or alpha-actin. In other embodiments, the animal protein is a subunit of a complex. In some of these embodiments, the complex is a myosin complex. The production of proteins by modified cells may be performed in multiple different cells, in some embodiments, the cyanobacteria cell is S. elongatus is PCC7942 or UTEX2973 or PCC7002, or Synechocystis sp. PCC6803. In some embodiments, multiple genes are incorporated into a cell. In some embodiments, each gene comprises a myosin complex subunit. The myosin complex subunits are MLC1, MLC2, and MYH2.

The invention discloses a local black pig pork flavor improvement method based on microbial fermentation, and relates to the technical field of microbial fermentation, and the method comprises the following specific steps: raw material pretreatment, enzymolysis treatment, low-salt pickling, sectional dynamic fermentation and post-treatment. The pork can be subjected to mild enzymolysis through neutral protease, micropores are formed in the surface of a pork matrix to expand microbial action sites, then the pork subjected to enzymolysis is treated through the compounded low-salt pickling liquid, salt in the low-salt pickling liquid can construct a low-permeability environment to inhibit infectious microbe colonization, and then the pork is subjected to enzymolysis; the white granulated sugar can provide a carbon source for subsequent strain metabolism, the composite phosphate can maintain the structural stability of muscle protein, the vitamin C can delay oxidation of myoglobin, the whole set of treatment process can achieve synchronous achievement of matrix modification, infectious microbe inhibition and matrix protection, and a stable early-stage foundation is built for subsequent fermentation. And the strain can start metabolism on a clean matrix with multiple acting sites.

The present disclosure relates to highly purified (R)-3′-hydroxybutyl (R)-3′-hydroxybutyrate and biomedical uses thereof. In particular highly purified (R)-3′-hydroxybutyl (R)-3′-hydroxybutyrate, is provided for enhancing bodily function and treating various disease states. The methods described herein provide for production of a highly purified (R)-3′-hydroxybutyl (R)-3′-hydroxybutyrate via processes external to the human body and administering effective amounts of highly purified (R)-3′-hydroxybutyl (R)-3′-hydroxybutyrate to human subjects to achieve a range of therapeutic benefits, including but not limited to, one or more of: inhibiting inflammasome activation, reducing pro-inflammatory cytokines, improving insulin sensitivity, enhancing mitochondrial biogenesis, and promoting muscle protein synthesis. Additionally, highly purified (R)-3′-hydroxybutyl (R)-3′-hydroxybutyrate is administered in an amount and frequency conducive to one or more of: improving cognitive function, reducing oxidative stress, enhancing cardiovascular health, and supporting neuroprotection. Methods for manufacturing and purifying (R)-3′-hydroxybutyl (R)-3′-hydroxybutyrate compositions to ensure high specificity and efficacy in therapeutic applications are also provided.

The application discloses application of alpha-ketobutyric acid in promoting transformation of skeletal muscle fiber type from fast muscle to slow muscle, and belongs to the technical field of animal nutrition. In the application, pig skeletal muscle satellite cells are treated by alpha-ketobutyric acid, mRNA expression levels of slow muscle genes MyHC I and MyHC IIa are significantly up-regulated, and expression level of fast muscle gene MyHC IIb is significantly down-regulated. Alpha-ketobutyric acid treatment significantly increases expression of slow muscle protein and reduces expression of fast muscle protein. In the myotube of the alpha-ketobutyric acid treatment group, MyHC-slow positive staining area is obviously higher than that of the control group, and MyHC-fast positive staining area is obviously lower than that of the control group. The above results show that the muscle fiber can be transformed from fast muscle to slow muscle after alpha-ketobutyric acid treatment. Therefore, the application provides a brand-new technical means and thought for regulating muscle physiology and improving meat quality.

The utility model discloses a fishy smell removal device in the aquatic product muscle protein enzymolysis process, and relates to the technical field of aquatic product muscle protein enzymolysis processing, the fishy smell removal device comprises an upper tank assembly and a purification assembly, the top of the upper tank assembly is connected with a sealing cover, and the purification assembly is installed on one side of the upper tank assembly; a deodorization assembly is installed on the side, away from the purification assembly, of the upper tank assembly. According to the fishy smell removing device in the aquatic muscle protein enzymolysis process, the fishy smell removing filter element and the fishy smell removing filter box are both filled with fishy smell removing filter materials, so that when an aquatic muscle protein solution is effectively purified and filtered, smell generated in enzymolysis of the aquatic muscle protein solution is rapidly extracted out of the whole device, and effective air filtration is carried out; meanwhile, through cooperation with operation of the purification assembly, the fishy smell in the solution can be removed as much as possible, the fishy smell is prevented from being deposited in the device to affect the quality of the enzymolysis aquatic product muscle protein solution, and meanwhile, the adverse effect caused by the fact that air in the device is directly exhausted to the outside is avoided.

The present invention relates to a composition for preventing or treating muscular diseases, or for improving muscular functions, containing gagnlioside. More specifically, the present invention relates to a pharmaceutical composition for preventing or treating muscular diseases, a food composition for preventing or alleviating muscular diseases, or a cosmetic composition for preventing or alleviating muscular diseases, all of which comprise gagnlioside, which can increase the expression of proteins associated with muscle protein synthesis and muscle mass growth in muscle cells and inhibit the expression of enzymes involved in muscle protein degradation at the mRNA level.

The present invention relates to a method for preparing peptone derived from a salmon by-product, and a medium composition, for culturing microorganisms, comprising the peptone. The method for preparing peptone derived from a salmon by-product comprises the steps of: heating a salmon by-product and pulverizing muscle proteins separated from bones; mixing the muscle protein powder with water to prepare a mixture; adding hydrolase to the mixture to hydrolyze same; and drying the hydrolyzed mixture to obtain peptone powder. In addition, the present invention provides peptone derived according to the present invention from a salmon by-product, and a medium composition comprising the peptone for culturing microorganisms, the peptone having skin regeneration, skin barrier strengthening, anti-aging, reverse aging, and antioxidant effects, and thus the present invention can be used in the commercial development of health functional foods, cosmetics, and pharmaceuticals, and can help reduce environmental pollution since fish waste is utilized.

The invention provides a probiotic fermentation composition capable of improving athletic performance and a preparation method of the probiotic fermentation composition, and belongs to the field of non-alcoholic beverages. The composition comprises the following components: a fermented red ginseng extract, a fermented radix astragali extract, a radix rehmanniae preparata extract, a radix salviae miltiorrhizae extract, a rhizoma chuanxiong extract, donkey-hide gelatin and a black tea extract. The composition prepared by a specific probiotic fermentation process has the following beneficial effects: firstly, through a multi-target synergistic effect, the motor function is systematically improved, including enhancing endurance, accelerating fatigue recovery and relieving motor-induced oxidative stress injury; 2, improving body energy metabolism and blood circulation, and promoting muscle protein synthesis and repair; thirdly, a process of firstly fermenting and then extracting is adopted, so that the bioavailability of active ingredients is remarkably improved, and the product is pure and safe; and 4, the preparation process is stable and controllable, and is suitable for physical maintenance and function improvement requirements of various groups such as athletes, middle-aged and elderly people, rehabilitation people and the like.

The invention discloses a crucian feed with soybean meal replaced by cottonseed protein concentrate and application of the crucian feed. Cottonseed protein concentrate is used as a main plant protein source and completely replaces soybean meal in a feed formula, and the mass percent of the cottonseed protein concentrate is 19.78%. The feed further comprises the following components: fish meal, casein, flour, fish oil, soybean oil, vitamins, mineral premix and the like. The feed provided by the invention can significantly improve the ingestion speed and the ingestion amount of crucian carps and effectively promote the growth of crucian carps, and the weight gain rate, the specific growth rate and the feed efficiency of the feed are significantly superior to those of a feed containing soybean meal. The feed can regulate and control lipid metabolism related gene expression of crucian muscle and liver, enhance fat synthesis and catabolism, promote muscle protein and fat deposition and increase muscle polyunsaturated fatty acid content, and has no negative influence on liver health and intestinal flora homeostasis. The invention provides an efficient solution for soybean meal decrement substitution, and has important application value.

The invention relates to liquid modified milk and a preparation method thereof, the liquid modified milk comprises animal milk, glucosamine hydrochloride, calcium beta-hydroxy-beta-methylbutyrate and cordyceps militaris extract, the cordyceps militaris extract contains cordycepin and adenosine, the cordycepin content in the liquid modified milk is greater than or equal to 1.6 mg / 100mL, and the adenosine content in the liquid modified milk is greater than or equal to 0.6 mg / 100mL. According to the modified milk provided by the invention, cordycepin enhances the biosynthesis and energy supply capability of mitochondria by activating related signal channels, and provides an energy basis for the GaHMB to play a role, so that the synthesis efficiency of muscle protein is improved; adenosine and glucosamine have a synergistic effect, so that conduction of inflammation-related signals can be blocked, and decomposition of cartilage matrixes is reduced; meanwhile, glucosamine is used as a glycosaminoglycan precursor, under the action of a signal channel regulated and controlled by adenosine, the synthesis efficiency of cartilage tissue can be remarkably stimulated, the utilization efficiency of sugar is improved, and finally triple synergistic intervention of muscle-joint-metabolism is achieved.

The invention relates to the field of microorganisms, and discloses a lactobacillus paracasei (Lactobacillus paracasei) and an application of the lactobacillus paracasei in prevention, alleviation or treatment of sarcopenia. The probiotics can promote muscle protein synthesis, inhibit protein degradation, improve mitochondrial functions and reduce visceral fat accumulation by regulating and controlling AKT / mTOR, NF-kB, FOXO3a and other signal channels, so that muscle mass decline, function decline and inflammatory state induced by sarcopenia are remarkably relieved. In addition, the lactobacillus paracasei improves metabolic health and inflammatory status by reducing the level of proinflammatory factors (such as TNF-alpha and IL-6) and up-regulating the expression of anti-inflammatory factors (such as IL-10). The traditional Chinese medicine composition is suitable for muscle loss caused by senile sarcopenia, obesity sarcopenia and heat limitation, has the advantages of clear mechanism, high safety, stable curative effect and the like, and provides a novel adjuvant therapy scheme for clinic.

The invention relates to an ACE inhibitory peptide as well as a screening method and application thereof, and belongs to the technical field of bioactive peptides. The invention provides an ACE inhibitory peptide. The amino acid sequence of the ACE inhibitory peptide is shown as RF, RW or KF. According to the method disclosed by the invention, turbot muscle protein is taken as a research object, virtual enzymolysis is utilized to assist activity prediction, preliminary screening is carried out by combining a bioinformatics method, polypeptide and an ACE three-dimensional structure are docked by adopting a semi-flexible molecular docking method, further screening is carried out, and the ACE inhibitory peptide is obtained. The ACE inhibition rates of the ACE inhibitory peptide obtained through screening are 76.61%, 80.71% and 79.18%, and compared with a method for extracting the ACE inhibitory peptide in the prior art, the ACE inhibitory peptide does not need enzymolysis, consumes less time and is lower in cost.

The present invention discloses a Chinese medicine for improving muscle atrophy caused by tumor cachexia, which has the effects of strengthening spleen, soothing liver, tonifying kidney and strengthening tendons, as well as a preparation method and application. The Chinese medicine includes the following raw materials: Chinese yam, white atractylodes, Pseudostellaria baicalensis, citron, bergamot, malt, liquorice, dendrobium, Chinese cyperus, papaya, millennium jian, and rhizoma dioscoreae. The results of in vivo and in vitro experiments show that the Chinese medicine prescription of the present invention has an improving effect on muscle atrophy in cachectic tumor-forming mice, and the mechanism may be that the level of inflammatory factors in myoblasts is inhibited, and the IL-6 / STAT3 / ubiquitin-protein autophagosome signaling pathway is affected, thereby reducing the degradation of muscle protein and the occurrence of autophagy, increasing the weight of mice, and effectively improving the symptoms of muscle atrophy caused by tumor malignancy; in terms of composition and proportion, the Chinese medicine prescription is adjusted according to the pathogenesis characteristics of tumor malignancy, making up for the limitations of Liujunzi Decoction, and effectively improving the muscle atrophy phenomenon in tumor malignancy.

The present disclosure relates to highly purified (R)-3′-hydroxybutyl (R)-3′-hydroxybutyrate and biomedical uses thereof. In particular highly purified (R)-3′-hydroxybutyl (R)-3′-hydroxybutyrate, is provided for enhancing bodily function and treating various disease states. The methods described herein provide for production of a highly purified (R)-3′-hydroxybutyl (R)-3′-hydroxybutyrate via processes external to the human body and administering effective amounts of highly purified (R)-3′-hydroxybutyl (R)-3′-hydroxybutyrate to human subjects to achieve a range of therapeutic benefits, including but not limited to, one or more of: inhibiting inflammasome activation, reducing pro-inflammatory cytokines, improving insulin sensitivity, enhancing mitochondrial biogenesis, and promoting muscle protein synthesis. Additionally, highly purified (R)-3′-hydroxybutyl (R)-3′-hydroxybutyrate is administered in an amount and frequency conducive to one or more of: improving cognitive function, reducing oxidative stress, enhancing cardiovascular health, and supporting neuroprotection. Methods for manufacturing and purifying (R)-3′-hydroxybutyl (R)-3′-hydroxybutyrate compositions to ensure high specificity and efficacy in therapeutic applications are also provided.

The invention discloses a bone defect repair material and a preparation method thereof. According to the invention, guanidine hydrochloride is utilized to change the spatial conformation of muscle protein and promote the structure of myofibril to become loose. After entering myofibril, Ca < 2 + > is complexed with troponin and serves as a nucleation site to induce nucleation growth of hydroxyapatite at a specific position, and ordered mineral stripes with the spacing being 1-2 microns can be formed after repeated circulating mineralization. The micron-sized stripe pattern is proved to be more beneficial to the adhesion and osteogenic differentiation of mesenchymal stem cells, and can accelerate the repair process of large-size bone defects. According to the method, the problem of insufficient autologous bone transplantation donors is solved, the self muscular tissue is used as a mineralization template, immunogenicity is not needed to be worried, sufficient mineral content and ordered mineral stripes are beneficial to adhesion, migration and growth of osteogenesis related cells, and the bone defect repair process can be remarkably accelerated.

The invention relates to the technical field of traditional Chinese medicine, in particular to a traditional Chinese medicine composition for improving muscle attenuation and preparation and application thereof. The traditional Chinese medicine composition for improving muscle attenuation comprises a rhodiola rosea extract, an American ginseng extract and a lucid ganoderma extract. The traditional Chinese medicine composition is beneficial to adjusting balance of muscle protein synthesis and decomposition, further protects muscle protein metabolism from two aspects of resisting inflammation and relieving insulin resistance, reduces damage to muscles by resisting oxidation and protecting mitochondrial functions, and can achieve the effects of maintaining and improving muscle quality and preventing and relieving muscle attenuation.