Water soluble animal muscle protein product
A technology of animal muscle and protein, applied in the direction of animal/human protein, muscle protein, mammalian protein, etc., can solve the problem of myofibrillar protein being insoluble in water, etc.
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Embodiment 1
[0026] Example 1: Hydrolysis at acidic pH
[0027] According to US Patent 6,005,073 (pH 2.8; 10,000 g force), a chicken protein isolate derived from myofibril protein and sarcoplasmic protein was produced from fresh chicken breast muscle and adjusted to pH 5.5 to precipitate the protein. Then hydrochloric acid (2N) was added dropwise to adjust the precipitated protein back to pH 3.5. Except for the acid, no other additives are added. The protein concentration of the acidified protein was 49.86 mg / ml. Put two aliquots of the protein sample into a glass beaker and put it in a 50°C water bath. Mix with a spatula in one of the beakers to disperse 0.05% (w / w) S-16774 Enzeco Fungal Acid Protease (Enzyme Development Corporation, 21 PennPlaza, 360 West 31 st St., New York, NY). The samples were incubated at 50°C for 2.3 hours. After that, sodium hydroxide (2N) was added dropwise to adjust both samples to pH 7.0. The sample without enzyme precipitated at pH 5.5, but returned to a liquid ap...
Embodiment 2
[0035] Example 2: Hydrolysis at neutral pH
[0036] According to US Patent 6,005,073 (pH 2.8; 10,000 g force), a chicken protein isolate derived from myofibril protein and sarcoplasmic protein was produced from fresh chicken breast muscle and adjusted to pH 5.5 to precipitate the protein. Then sodium hydroxide (2N) was added dropwise to adjust the precipitated protein to pH 7. Except for the acid, no other additives are added. Put two aliquots of the protein sample into a glass beaker and put it in a 40°C water bath. Mix with a spatula in one of the beakers to disperse 0.2% (w / w) Neutrase 0.8L (Batch PWNO 1208; Novozymes A / S, Krogshoejvej 36, 2880 Bagsvaerd, Denmark). The samples were incubated at 40°C for 0.5 hours. Both samples were then placed in the refrigerator before measuring viscosity and solubility.
[0037] Feature
Add enzyme
Without enzyme
Viscosity (cps.) 1
9.4
65.9
Solubility (%) 2
100
13.3
[0038] 1 Bri...
Embodiment 3
[0040] Example 3: Hydrolysis at alkaline pH
[0041] According to US Patent 6,005,073 (pH 2.8; 10,000 g force), a chicken protein isolate derived from myofibril protein and sarcoplasmic protein was produced from fresh chicken breast muscle and adjusted to pH 5.5 to precipitate the protein. Then sodium hydroxide (2N) was added dropwise to adjust the precipitated protein to pH 8.0. Except for the acid, no other additives are added. Put two aliquots of the protein sample into a glass beaker and put it in a 55°C water bath. Mix with a spatula in one of the beakers to disperse 0.5% (w / w) Alcalase 2.4 L FG, (Batch PLN05212; Novozymes A / S, Krogshoejvej 36, 2880 Bagsvaerd, Denmark). The samples were incubated at 55°C for 1.5 hours. The two samples were then adjusted to pH 7 and placed in the refrigerator before measuring viscosity and solubility.
[0042] Feature
Add enzyme
Without enzyme
Viscosity (cps.) 1
1.9
72.8
Solubility (%) 2
99.4 ...
PUM
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