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Special culture device for 3D biological tissues and preparation method of blocky cultured meat

A biological tissue and culture device technology, applied in tissue cell/virus culture devices, biochemical equipment and methods, 3D culture, etc., to achieve the effect of less reagent consumption, high cell growth density, and uniform growth and differentiation.

Active Publication Date: 2021-01-22
FOODSTUFF INST BEIJING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The embodiment of the present invention provides a special culture device for 3D biological tissue and a method for preparing block cultured meat. By solving technical problems such as 3D culture of animal skeletal muscle satellite cells, it overcomes the current inability to prepare block with a larger size at one time. Defects of a single structural unit of cultured meat, the control of the 3D configuration and size of its single structural unit can be achieved during the preparation of cultivated meat

Method used

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  • Special culture device for 3D biological tissues and preparation method of blocky cultured meat
  • Special culture device for 3D biological tissues and preparation method of blocky cultured meat
  • Special culture device for 3D biological tissues and preparation method of blocky cultured meat

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Embodiment 1

[0059] The embodiment of the present invention provides a special culture device for 3D biological tissue, its structure schematic diagram is as follows figure 1 shown, including:

[0060] 3D biological tissue culture tank 1, used to place 3D biological tissue,

[0061] And the liquid storage tank 3, is used for containing culture medium;

[0062] The 3D biological tissue culture tank 1 and the liquid storage tank 3 are connected by pipelines to form a circulation loop for the medium to circulate; the pipeline flowing into the 3D biological tissue culture tank 1 is provided with a first differential pressure gauge 5 and a perfusion pump 6, The pipeline flowing out from the 3D biological tissue culture tank 1 is provided with a second differential pressure gauge 7 and a suction pump 8;

[0063] The opening of the 3D biological tissue culture tank 1 is provided with a sealing plug 2, and the inner side of the sealing plug 2 is provided with a plurality of medium perfusion need...

Embodiment 2

[0065] The embodiment of the present invention provides a kind of preparation method of bulk pig cultured meat, specifically as follows:

[0066] (1) Extraction of porcine skeletal muscle satellite cells

[0067] Newborn pigs were treated with CO 2 Put it to death, soak it in 75% alcohol solution for 5 minutes, then dissect the pig to obtain the skeletal muscle tissue of its thigh, tear off the fascia tissue, and cut it into about 1mm with ophthalmic scissors 3 square tissue block; then add phosphate buffered saline solution (PBS) pipette gun to gently wash the tissue block, centrifuge at 700rpm for 5min after completion, absorb the supernatant, and the lower sediment is the porcine skeletal muscle tissue block; then use 20 % fetal calf serum in DMEM medium, resuspend the above tissue pieces and spread them on a petri dish at 37°C, 5% CO 2 Cultured in an incubator under static conditions. After culturing for 3 days, suck out the porcine skeletal muscle tissue pieces in the ...

Embodiment 3

[0080] The embodiment of the present invention provides a kind of preparation method of nugget chicken cultured meat, specifically as follows:

[0081] (1) Extraction of chicken skeletal muscle satellite cells

[0082] Select well-developed eggs with no damage to the egg shell and no obvious unremovable dirt. Gently wipe and disinfect with 75% alcohol cotton balls. Use medical tweezers to gently tap the air chamber and remove the white film attached to the shell. Take out the chicken embryo with tweezers and soak it in 75% alcohol for 3 seconds. Then put the sterilized chicken embryos into a plate poured with PBS solution in advance, rinse twice with PBS solution; absorb the PBS solution, cut off the thigh of the chicken embryo, tear off the outer fascia, and fix it with a pair of tweezers Muscle, a pair of curved tweezers tore and pulled the muscle, tearing the muscle attached to the bone on one thigh of the chicken embryo into a minced shape. Add PBS submerged in the muscl...

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Abstract

The embodiment of the invention provides a special culture device for 3D biological tissues and a preparation method of blocky cultured meat. The special culture device for 3D biological tissues comprises a 3D biological tissue culture tank used for placing 3D biological tissues, and a liquid storage tank used for containing a culture medium; the 3D biological tissue culture tank and the liquid storage tank are connected through a pipeline to form a circulation loop for the culture medium to circularly flow; a sealing plug is arranged at an opening of the 3D biological tissue culture tank; anda plurality of culture medium filling needles are arranged on the inner side of the sealing plug, and the culture medium filling needles penetrate through 3D biological tissues during use. The special culture device for the 3D biological tissues can effectively guarantee uniform growth and differentiation of cells in the 3D biological tissues, and is suitable for preparing blocky cultured meat. According to the preparation method of the blocky cultivated meat, the preparation scale of the animal cultivated meat can be remarkably increased, the high-quality blocky cultivated meat is truly prepared, and the blocky cultivated meat has the chewing taste of meat products.

Description

technical field [0001] The invention relates to the technical field of cultured meat preparation, in particular to a special culture device for 3D biological tissue and a method for preparing block cultured meat. Background technique [0002] Cultivated meat, also known as cultured meat, clean meat, in vitro meat, etc., is a kind of artificial meat based on cell production. Because this type of meat can bypass animal feeding and provide humans with real animal protein sustainably, it is considered It is the most likely solution to the plight of human meat production and consumption in the future. See Wang Shouwei's "Category and Nomenclature Analysis and Specification Suggestions for Artificial Meat" (Food Science, 2020, 41(11): 310-316). [0003] At present, the preparation technology of cultured meat is still in the development stage, and many key technologies such as the source of cells required for its preparation, in vitro cell culture, and in vitro 3D molding of cells...

Claims

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Application Information

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IPC IPC(8): C12M3/00C12N5/077B33Y80/00
CPCC12M21/08C12M29/10C12M29/18C12N5/0659C12N5/0658B33Y80/00C12N2533/54C12N2533/78C12N2513/00C12N2501/11C12N2501/33B33Y30/00B33Y10/00C12M25/14
Inventor 王守伟李石磊李莹莹刘文婷李雨爽
Owner FOODSTUFF INST BEIJING
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