A special culture device for 3D biological tissue and a method for preparing block cultured meat

A biological tissue and culture device technology, applied in tissue cell/virus culture devices, biochemical equipment and methods, tissue culture, etc., to achieve the effects of large expansion potential, high cell growth density, and low reagent consumption

Active Publication Date: 2022-06-07
FOODSTUFF INST BEIJING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The embodiment of the present invention provides a special culture device for 3D biological tissue and a method for preparing block cultured meat. By solving technical problems such as 3D culture of animal skeletal muscle satellite cells, it overcomes the current inability to prepare block with a larger size at one time. Defects of a single structural unit of cultured meat, the control of the 3D configuration and size of its single structural unit can be achieved during the preparation of cultivated meat

Method used

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  • A special culture device for 3D biological tissue and a method for preparing block cultured meat
  • A special culture device for 3D biological tissue and a method for preparing block cultured meat
  • A special culture device for 3D biological tissue and a method for preparing block cultured meat

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Embodiment 1

[0059] The embodiment of the present invention provides a special culture device for 3D biological tissue, the schematic diagram of which is as follows: figure 1 shown, including:

[0060] 3D biological tissue culture tank 1 for placing 3D biological tissue,

[0061] and liquid storage tank 3 for holding culture medium;

[0062] The 3D biological tissue culture tank 1 and the liquid storage tank 3 are connected by pipelines to form a circulation loop for the circulation of the culture medium; the pipeline flowing into the 3D biological tissue culture tank 1 is provided with a first differential pressure gauge 5 and a perfusion pump 6, A second differential pressure gauge 7 and a suction pump 8 are provided on the pipeline flowing out of the 3D biological tissue culture tank 1;

[0063] The opening of the 3D biological tissue culture tank 1 is provided with a sealing plug 2, and the inner side of the sealing plug 2 is provided with a plurality of culture medium perfusion need...

Embodiment 2

[0065] The embodiment of the present invention provides a method for preparing a lumpy pig cultured meat, which is specifically as follows:

[0066] (1) Extraction of porcine skeletal muscle satellite cells

[0067] Newborn pigs are first treated with CO 2 The pigs were sacrificed, soaked in 75% alcohol solution for 5 minutes, and then dissected the pigs to obtain the skeletal muscle tissue in the thighs. After tearing off the fascia tissue, use ophthalmic scissors to cut it into about 1 mm. 3 Square tissue block; then add phosphate buffered solution (PBS) pipette to gently wash the tissue block, after completion, centrifuge at 700rpm for 5min, aspirate the supernatant, and the lower sediment is the pig skeletal muscle tissue block; DMEM medium with % fetal bovine serum The above tissue pieces were resuspended and plated in petri dishes at 37°C, 5% CO 2 Incubate in an incubator under conditions. After culturing for 3 days, the porcine skeletal muscle tissue block in the pet...

Embodiment 3

[0080] The embodiment of the present invention provides a method for preparing a piece of chicken meat, which is specifically as follows:

[0081] (1) Extraction of chicken skeletal muscle satellite cells

[0082] Select eggs that are well-developed, with no damage to their shells, and no obvious dirt that cannot be removed. Gently wipe and disinfect them with 75% alcohol cotton balls. Use medical tweezers to gently break the air chamber and lift off the albuginea attached to the shell. Remove the chicken embryos with tweezers and soak them in 75% alcohol for 3 seconds. Then put the sterilized chicken embryos into a plate with PBS solution poured in advance, rinse with PBS solution twice; suck off the PBS solution, cut off the thigh part of the chicken embryo, tear off the outer fascia, and fix it with a pair of tweezers Muscle, an elbow tweezers tear the muscle, tearing the muscle attached to the bone on one thigh of the chicken embryo into a powder. Add PBS without muscle ...

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Abstract

Embodiments of the present invention provide a special 3D biological tissue culture device and a method for preparing block-shaped cultured meat. The special 3D biological tissue culture device includes: a 3D biological tissue culture tank for placing 3D biological tissue, and a liquid storage tank. It is used to hold the medium; the 3D biological tissue culture tank and the liquid storage tank are connected by pipelines to form a circulation loop for the medium to circulate; the opening of the 3D biological tissue culture tank is provided with a sealing plug, and the sealing The inner side of the plug is provided with a plurality of medium perfusion needles which pass through the 3D biological tissue in use. The special culture device for 3D biological tissue provided by the present invention can effectively ensure the uniform growth and differentiation of cells inside the 3D biological tissue, and is suitable for preparing block cultured meat. The preparation method of the block cultured meat provided by the invention can significantly increase the scale of preparation of animal cultured meat, and truly realize the preparation of high-quality block cultured meat, which has the chewing taste of meat products.

Description

technical field [0001] The invention relates to the technical field of cultured meat preparation, in particular to a 3D biological tissue-specific culture device and a preparation method of a block-shaped cultured meat. Background technique [0002] Cultivated meat, also known as cultured meat, clean meat, in vitro meat, etc., is a kind of artificial meat produced based on cells. This type of meat can supply real animal protein to humans sustainably because it can bypass animal feeding. It is the most likely solution to the predicament of human meat production and consumption in the future. See Wang Shouwei's "Analysis and Normative Recommendations for Classification and Naming of Artificial Meat" (Food Science, 2020, 41(11): 310-316). [0003] At present, the preparation technology of cultured meat is still in the development stage, and many key technologies such as cell source, in vitro cell culture, and in vitro 3D molding of cells required for its preparation still need...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M3/00C12N5/077B33Y80/00
CPCC12M21/08C12M29/10C12M29/18C12N5/0659C12N5/0658B33Y80/00C12N2533/54C12N2533/78C12N2513/00C12N2501/11C12N2501/33
Inventor 王守伟李石磊李莹莹刘文婷李雨爽
Owner FOODSTUFF INST BEIJING
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