Method for scalable skeletal muscle lineage specification and cultivation
A cell line and culture medium technology, applied in biochemical equipment and methods, botany equipment and methods, bone/connective tissue cells, etc., can solve problems such as inability to fully solve the dependence of cultured meat production
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment
[0081] method
[0082] O2KM cell line. The O2KM cell line was obtained from the parental O2K cell line by lentiviral insertion of blasticidin (an optional transgenic cassette containing MyoDER open reading frame sequence (ORF)). As mentioned in this exemplary section, O2K cells / cell lines and piPSC cells can be used interchangeably. Prepare as follows Figure 4A The lentiviral vector described in: cloned from the pENTR / D-TOPO entry vector (Life Technologies#K2435-20) containing the PCR-amplified MyoDER ORF, and cloned the pLentiCMVBlast destination vector between the attR1 and attR2 recombination sites ( MyoDER ORF (Addgene#13494) downstream of the CMV promoter of Addgene#17451). To prepare the lentiviral supernatant, use PolyJet transfection reagent (Signagen#SL100688) to combine 293FT cells with the prepared pLentiCMVBlast[MyoDER] plasmid, pMD2.G envelope plasmid (Addgene#12259) and psPAX2 packaging plasmid (Addgene#12260) ) Co-transfection. O2K was transduced with pseudovi...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com