Method for scalable skeletal muscle lineage specification and cultivation

A cell line and culture medium technology, applied in biochemical equipment and methods, botany equipment and methods, bone/connective tissue cells, etc., can solve problems such as inability to fully solve the dependence of cultured meat production

Inactive Publication Date: 2016-08-24
UNIV OF MISSOURI BOARD OF CURATORS +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Thus, current approaches do not adequately address the animal dependence of cultured meat productio

Method used

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  • Method for scalable skeletal muscle lineage specification and cultivation
  • Method for scalable skeletal muscle lineage specification and cultivation
  • Method for scalable skeletal muscle lineage specification and cultivation

Examples

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Embodiment

[0081] method

[0082] O2KM cell line. The O2KM cell line was obtained from the parental O2K cell line by lentiviral insertion of blasticidin (an optional transgenic cassette containing MyoDER open reading frame sequence (ORF)). As mentioned in this exemplary section, O2K cells / cell lines and piPSC cells can be used interchangeably. Prepare as follows Figure 4A The lentiviral vector described in: cloned from the pENTR / D-TOPO entry vector (Life Technologies#K2435-20) containing the PCR-amplified MyoDER ORF, and cloned the pLentiCMVBlast destination vector between the attR1 and attR2 recombination sites ( MyoDER ORF (Addgene#13494) downstream of the CMV promoter of Addgene#17451). To prepare the lentiviral supernatant, use PolyJet transfection reagent (Signagen#SL100688) to combine 293FT cells with the prepared pLentiCMVBlast[MyoDER] plasmid, pMD2.G envelope plasmid (Addgene#12259) and psPAX2 packaging plasmid (Addgene#12260) ) Co-transfection. O2K was transduced with pseudovi...

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Abstract

The present disclosure relates to methods for enhancing cultured meat production, such as livestock-autonomous meat production. In certain aspects, the meat is any metazoan tissue or cell-derived comestible product intended for use as a comestible food or nutritional component by humans, companion animals, domesticated or captive animals whose carcasses are intended for comestible use, service animals, conserved animal species, animals used for experimental purposes, or cell cultures.

Description

[0001] Cross references to related applications [0002] This application claims the benefits of U.S. Provisional Patent Application 61 / 962,068 filed on October 30, 2013, which is incorporated herein by reference in its entirety. [0003] Incorporation of Sequence Listing [0004] The electronically filed ASCII text file (name 52553_136621_ST25.txt; size: 2,962 bytes; and creation date: October 30, 2014) submitted with this application is incorporated by reference in its entirety. In this article. [0005] Statement on Federally Funded Research and Development [0006] The present invention was completed with government support under the license number R01HD069979 / 00032722 granted by the National Institutes of Health. The government has certain rights in this invention. Background technique [0007] The present disclosure relates to methods for enhancing cultivated meat production such as autonomous meat production by livestock. "Cultivated meat" (such as in vitro cell culture, tiss...

Claims

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Application Information

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IPC IPC(8): A01N63/00A01N65/00A61K35/34A61K35/57
CPCC12N2501/392C12N2501/727C12N2501/999A61K35/28A61K35/545A23L13/00C12N5/10C12N2501/415C12N2501/603C12N2501/604C12N2501/72C12N2506/02C12N5/0658C12N2501/60C12N2506/45C12N2501/602A23V2002/00C12N2501/40
Inventor N·J·吉诺维斯R·M·罗伯茨B·P·V·L·泰卢固
Owner UNIV OF MISSOURI BOARD OF CURATORS
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