Serum-free culture system and application thereof in cultured meat

A serum-free culture and system technology, applied in the field of bioengineering, can solve the problems of low safety and high cost, and achieve the effects of avoiding pollution, reducing high cost and clear components.

Pending Publication Date: 2021-01-12
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the development of animal cell serum-free culture technology, serum-free culture has been widely used in the field

Method used

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  • Serum-free culture system and application thereof in cultured meat
  • Serum-free culture system and application thereof in cultured meat
  • Serum-free culture system and application thereof in cultured meat

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1 serum-free culture system composition

[0025] Table 1 Basal medium

[0026]

[0027] Table 2 Exogenously Added Supplementary Components

[0028]

[0029] The serum-free culture system includes basal medium (Table 1) and exogenously added supplementary components (Table 2).

[0030] 1~5×10 4 Individual BHK21 cells (suckling hamster kidney cells) were inoculated in 2mL serum-free culture system, then cultured aseptically at 19-24°C for 48 hours, counted the cells by quantitative method, and evaluated the serum-free culture system. The results are as follows: the cell morphology is plump, Stable; normal cell growth numbers can be achieved over the desired growth cycle.

[0031] The composition of the basal medium remains unchanged (see Table 1), and the content of different components in the supplementary components added by exogenous sources is adjusted, and the culture system of group A, group B, group C, group D and group E is set to cultivate BHK...

Embodiment 2

[0035] Example 2 Application of serum-free culture system in the production of artificial meat

[0036]In the invention, exogenous synthetic cell growth factors, phospholipid growth factors, transferrin, amino acids, vitamins and other substances are added and compounded with basic medium to form a new type of serum-free medium. The culture system can rapidly proliferate muscle satellite cells (muscle stem cells). The medium was changed every 6-12 hours, and the cells were cultured for 48 hours at 19-24°C. With DMEM+5% newborn bovine serum medium (Gibco TM Advanced DMEM, 12491015) has the same effect. Therefore, the serum-free medium in the present invention can be used to completely replace the serum-containing medium, and different animal cells can be cultured on a large scale at low cost, and applied to animal cell culture meat.

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Abstract

The invention discloses a serum-free culture system and application thereof in cultured meat, and belongs to the technical field of bioengineering. The serum-free culture system can reduce dependenceof animal cell culture process on serum, and reduce influence of uncertain factors in serum on safety of animal cell culture. In addition, all exogenous additive components except basic culture mediumused in the serum-free culture system are completely synthesized, so that cost of the serum-free culture system can be effectively reduced, and large-scale application of the serum-free culture system is facilitated.

Description

Technical field [0001] The invention relates to a serum-free culture system and its application in cell cultured meat, and belongs to the technical field of bioengineering. Background technique [0002] In the traditional cell culture stage, animal serum is used to provide nutrients and biological factors required for cell attachment, proliferation and maintenance of growth. Since the animal serum used in batches is derived from natural organisms, it carries and is collected and processed, and there are contaminants from foreign microorganisms and The possibility of pathogenic factors poses biosecurity risks. In addition, due to factors such as complex serum components, poor batch-to-batch stability, limited supply, and high purification difficulty, serum-containing culture systems cannot achieve large-scale industrial application. [0003] Serum-free medium is a synthetic medium that can maintain cell growth and proliferation in vitro for a long time without adding serum. ...

Claims

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Application Information

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IPC IPC(8): C12N5/00C12N5/0775
CPCC12N5/0037C12N5/0662C12N2501/10C12N2500/24C12N2500/36C12N2500/32C12N2500/38C12N2500/90
Inventor 张国强陈坚周景文堵国成赵鑫锐李雪良
Owner JIANGNAN UNIV
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