Manufacturing method of edible sodium alginate/gelatin 3D gel scaffold adopting tea polyphenol coating for cultured meat

Abstract

The invention relates to a manufacturing method of an edible sodium alginate / gelatin 3D gel scaffold adopting a tea polyphenol coating for cultured meat, the 3D gel scaffold manufactured by the method can be used for proliferation, differentiation and culture of skeletal muscle stem cells, which provides organized support for the cultured meat, and belongs to the field of future food science and technology. The method comprises the following steps: uniformly mixing sodium alginate and gelatin with a certain concentration according to a certain ratio, fully reacting at room temperature for more than 2 hours, refrigerating overnight, and soaking in a calcium chloride / tea polyphenol solution to obtain the sodium alginate / gelatin 3D gel scaffold coated by the tea polyphenol. The manufacturing method is simple and efficient, and no toxic cross-linking agent is used. The obtained sodium alginate / gelatin 3D gel scaffold with the tea polyphenol coating has good cell adhesion and compatibility, and can be used for proliferation, differentiation and culture of skeletal muscle stem cells. The invention provides a manufacturing method of a novel and edible 3D scaffold for the research of cultured meat.

Description

technical field [0001] The invention relates to the field of future food science and technology, in particular to a method for manufacturing an edible sodium alginate / gelatin 3D gel support coated with tea polyphenols for cell cultured meat. Background technique [0002] As one of the main sources of high-quality protein, meat is an indispensable food on the table of modern people. However, due to problems such as low resource utilization, environmental pollution, and zoonotic foodborne diseases, consumers are paying more and more attention to the safety of livestock and poultry meat. Artificial meat has traceable production and processing sources, food safety and Advantages such as green sustainability are potential solutions to reduce the adverse effects of the current meat production system. [0003] Cultured meat, a field of cellular agriculture, is being considered as a novel alternative protein source. Furthermore, this material can serve as functional food by tailor...

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to address the problems of high research and development costs and the inability to realize large-scale production of existing cell cultured meat. Foot, providing an edible sodium alginate / gelatin 3D gel scaffold coated with tea polyphenols for cell culture meat Manufacturing method

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