24 results about "Gelatin gel" patented technology

The invention relates to a composite bone repair material, a preparation method and application, the preparation method comprises the following steps: immersing silkworm cocoons in a soap salt solution for degumming, drying to prepare degummed silk, dissolving the degummed silk in a lithium bromide solution, dialyzing for desalting, and concentrating to obtain a regenerated silk protein solution; mixing the regenerated silk fibroin solution with a gelatin solution to obtain a gel solution; mechanically shaking the gel solution, so that the gel solution is uniformly mixed, uniformly distributed bubbles are formed in the gel solution, and a silk protein / gelatin gel block is obtained; soaking and cleaning the silk protein / gelatin gel block in an ammonium sulfate solution to obtain silk protein / gelatin cross-linked gel; and freeze-drying the fibroin / gelatin cross-linked gel to obtain the fibroin / gelatin composite material. The composite bone repair material has the advantages that the composite bone repair material has a bionic porous structure, has excellent mechanical properties and also has good biocompatibility and osteogenic activity. The material can be applied to filling and regeneration repair of skull defects of the orthopedics department, the oral cavity, the plastic surgery (maxillofacial) and the neurosurgery department, and has high industrial production and clinical application prospects.

The invention discloses a high-stability and low-sensitization 3D cell culture meat scaffold and a preparation method thereof, and belongs to the field of future food science and technology processing. The fish gelatin sodium alginate cell culture meat scaffold is prepared through covalent modification of sodium alginate with different concentrations, the problem that fish gelatin gel is unstable at 37 DEG C is remarkably improved, the gel strength is improved by about 282.42%, the hydrophilicity is remarkably improved, and the cell culture meat scaffold is suitable for being used for preparing the fish gelatin sodium alginate cell culture meat scaffold. Meanwhile, the release amount of mouse plasma histamine of intragastric sodium alginate covalently modified group fish gelatin is reduced by about 54.35%, and the release rate of beta-hexosaminidase of RBL-2H3 cells is reduced from 27.50% to 11.31%. The high-stability and low-sensitization cell culture meat scaffold is prepared from fish gelatin, sodium alginate and calcium chloride, the problem of low stability of a single fish gelatin cell scaffold is solved, and the potential sensitization risk of the cell culture meat scaffold is reduced.

The invention provides a probiotic mixed fermentation method for enhancing a synergistic effect. Lactobacillus plantarum, bifidobacterium longum and clostridium tyrobutyricum are inoculated according to a volume ratio, and a pH-triggered two-stage fermentation process is adopted: the first stage is micro-aerobic to drive lactic acid bacteria to quickly produce acid, and the second stage is anaerobic to directionally improve butyric acid synthesis by pulse feeding of inulin-cysteine sustained-release particles. Physical isolation of flora is realized by using a double-layer gradient carrier, lactic acid bacteria are immobilized by sodium alginate-gelatin gel on the outer layer to form an acid barrier, and bifidobacterium and butyric acid producing bacteria are co-immobilized by chitosan microspheres on the inner layer to construct an anaerobic microenvironment. The technology breaks through the competitive inhibition bottleneck of multi-strain fermentation, the total number of viable bacteria is increased by 150% compared with a traditional technology, the yield of butyric acid is increased by 355%, and the technology is suitable for large-scale production of functional food and pharmaceutical preparations.

The invention relates to the field of biological medicine, and discloses eutectic gel based on protein as well as a preparation method and application of the eutectic gel. The eutectic gel based on the protein comprises the following components: gelatin, an antibacterial component and a deep eutectic solvent, and the Gel-COS-Ed eutectic gel comprises the following components: gelatin, chitosan oligosaccharide, emodin, glycerol and betaine. Heating and dissolving gelatin and antibacterial components, and solidifying to obtain gelatin gel; and placing the gelatin gel in a deep eutectic solvent to obtain the eutectic gel based on the protein. The tensile strength of the obtained Gel-COS-Ed eutectic gel is 1.01 MPa, the fracture strain can reach 510%, the Gel-COS-Ed eutectic gel has no obvious phase change in the temperature range of-80 DEG C to 80 DEG C, the bacteriostasis rate on staphylococcus aureus reaches 95.7%, and a new solution is provided for application of wound dressings and biomedical materials.

The invention discloses a fish gelatin jelly gel performance evaluation method based on multi-scale correlation analysis, which comprises the following steps: extracting multi-scale characteristics of molecular characteristics, network structures and macro performance of three fish gelatin jellies, performing data correlation based on three dimensions of molecular characteristics-network structures-macro performance, and combining a stoichiometric analysis method to evaluate the gel performance of the fish gelatin jellies. And a multi-level systematic evaluation system is constructed. According to the method, data of molecular characteristics (secondary structure ratio, particle size, Zeta potential and amide A wave number), network structure (aperture size) and macroscopic performance (imino acid content and gel strength) are integrated to construct the relevance of molecular characteristics-network structure-macroscopic performance, and full-dimensional analysis of the quality of the fish gelatin jelly is realized for the first time. The method breaks through the limitation that the traditional method only depends on single indexes such as protein or collagen content and the like, and clarifies the regulation and control of molecular aggregation behavior, imino acid content and electrostatic interaction on the formation of a gel network.

In order to improve the absorption efficiency of skin to mask liquid, the mask capable of achieving deep transdermal absorption is designed, the method for preparing the mask capable of achieving deep transdermal absorption according to the absorption efficiency factor of mask materials can achieve the purpose that traditional Chinese medicine paste is screened through transdermal agents, and the purpose of deep transdermal absorption is achieved. By adopting more than 20 materials such as propylene glycol, azone, gelatin gel, polypropylene glycol, asiaticosine, menthol, berberine hydrochloride, sodium alginate and the like, the prepared permeation and absorption mask has relatively strong effects of promoting skin permeation and absorption, healing wounds, resisting allergy and resisting inflammation.

The invention discloses a synthetic method of gelatin sponge particles. The synthetic method comprises the following steps: preparing a gelatin solution; adding a water-soluble developing agent, and uniformly stirring to obtain gelatin gel; drying and crushing to obtain pretreated gelatin powder; putting the pretreated gelatin powder and water into a high-pressure reaction kettle together, and uniformly stirring to obtain gelatin dispersion liquid; adding a cross-linking agent and a physical foaming agent into the gelatin dispersion liquid to increase the pressure in the reaction kettle to a first pressure; stopping stirring and releasing the pressure in the high-pressure reaction kettle to prepare the gelatin sponge particles. According to the method provided by the invention, firstly, gelatin gel with a developing effect is prepared, and then the gelatin gel is dried and crushed to obtain pretreated gelatin powder with uniform particles, so that the gelatin powder, a cross-linking agent and a physical foaming agent are mixed in water and then cross-linked to form developable gelatin sponge particles with relatively uniform particle sizes; the problems that in the background technology, foam structures are prone to being damaged, and the particle morphology is uncontrollable when gelatin sponge particles are manufactured are solved.

The invention discloses a preparation method of polyphenol-polysaccharide synergistically-improved thermally-stable fish gelatin gel and 3D printing application of the polyphenol-polysaccharide synergistically-improved thermally-stable fish gelatin gel. According to the method, tannic acid and sodium carboxymethyl cellulose are introduced into a fish gelatin system and are fully stirred and mixed, and tannic acid as a cross-linking agent interacts with sodium carboxymethyl cellulose and fish gelatin to construct a more compact three-dimensional gel network, so that the preparation of the polyphenol and polysaccharide synergistically enhanced fish gelatin-based gel is realized. The gel shows excellent printing adaptability and structural stability in 3D printing application, geometric shapes can be accurately formed, the gel has wide application prospects in the fields of personalized food design, biological material manufacturing and the like, and a new technical path is provided for high-value development of fish gelatin.

The invention relates to the technical field of medicines, in particular to sound-induced piezoelectric hydrogel as well as a preparation method and application thereof. The method comprises the following steps: 1) synthesizing a nano-plate by adopting a hydrothermal method; or in the process of synthesizing the nano-plate by the hydrothermal method, adding an iron source into the reaction liquid, and carrying out hydrothermal reaction to obtain the iron-doped nano-plate, (2) carrying out lamellar stripping on the nano-plate to obtain a nano-sheet; and 3) preparing gelatin gel, and then mixing the gelatin gel with the nanosheets obtained in the step 2) to obtain the sound-induced piezoelectric hydrogel. The piezoelectric-like material is innovatively applied to treatment of infected wounds, the piezoelectric-like material serves as a novel piezoelectric material, the acoustic heating effect can be rapidly generated under low-power ultrasonic induction so as to treat the biological membrane infected wounds, and in addition, the temperature rise can promote wound healing by increasing the blood flow volume, promoting cell migration and the like.

The invention discloses a probiotic-embedded cucumber fruit hydrogel bead and a preparation method thereof, and belongs to the technical field of embedding of probiotics. The preparation method of the probiotic-embedded cucumber pectin hydrogel beads comprises the following steps: 1) extracting cucumber pectin; (2) preparing a cucumber pectin-sodium alginate-gelatin gel solution; (3) preparing freeze-dried bacterial powder of lactobacillus rhamnosus and lactobacillus plantarum; and 4) uniformly mixing the freeze-dried bacterial powder with the gel solution, and dropwise adding the obtained mixed solution into CaCl2 to prepare the hydrogel beads. According to the preparation method disclosed by the invention, the protected lactobacillus rhamnosus and lactobacillus plantarum freeze-dried bacterial powder are embedded by the hydrogel beads prepared from the extracted cucumber pectin, the sodium alginate and the gelatin, and the hydrogel beads and the strain protective agent perform double protection on the lactic acid bacteria, so that the lactic acid bacteria have good acid resistance, digestion resistance and storability.

The present invention discloses a method for synthesizing gelatin sponge particles, comprising the following steps: preparing a gelatin solution; adding a water-soluble developer and stirring uniformly to obtain a gelatin gel; drying and crushing the solution to obtain a pretreated gelatin powder; placing the pretreated gelatin powder and water in an autoclave and stirring uniformly to obtain a gelatin dispersion; adding a crosslinking agent and a physical foaming agent to the gelatin dispersion to raise the pressure in the autoclave to a first pressure; stopping stirring and releasing the pressure in the autoclave to obtain gelatin sponge particles. The method provided by the present invention first prepares a gelatin gel with a developing function, then dries and crushes the gelatin gel to obtain a pretreated gelatin powder with uniform particles, and then mixes the gelatin powder, the crosslinking agent, and the physical foaming agent in water and crosslinks to form developable gelatin sponge particles with relatively uniform particle size. This method solves the problems of the prior art in which the production of gelatin sponge particles easily causes the destruction of the pore structure and the uncontrollable particle morphology.

The application relates to the field of biological medicine, and discloses a protein-based co-crystal gel as well as a preparation method and application thereof. The protein-based co-crystal gel comprises gelatin, an antibacterial component and a deep eutectic solvent, wherein the components of the Gel-COS-Ed co-crystal gel comprise gelatin, chitosan oligosaccharide, emodin, glycerol and betaine. After the gelatin and the antibacterial component are heated and dissolved, the gelatin is coagulated into a gelatin gel; the gelatin gel is placed in the deep eutectic solvent to obtain the protein-based co-crystal gel. The tensile strength of the obtained Gel-COS-Ed co-crystal gel is 1.01 MPa, the breaking strain can reach 510%, there is no obvious phase change in the temperature range of-80 DEG C to 80 DEG C, the antibacterial rate on staphylococcus aureus reaches 95.7%, and a new solution is provided for the application of wound dressings and biological medical materials.

The present application provides a gelatin particle and a preparation method thereof, and a hemostatic fluid gelatin composition. The gelatin particle is a cross-linked gelatin particle, and the preparation raw material of the gelatin particle comprises gelatin and a high molecular material containing amino and / or carboxyl; the preparation process of the gelatin particle comprises the steps of forming a gelatin gel from the gelatin and crushing the gelatin gel, wherein the high molecular material is added to mix with the solution of the gelatin or the gelatin gel before or after the formation of the gelatin gel. The gelatin particle and the hemostatic fluid gelatin of the present application can quickly absorb the moisture in the blood after being applied to a bleeding wound, thereby increasing the concentration of red blood cells, blood coagulation factors and the like in the blood, accelerating the endogenous blood coagulation mechanism, and having a good hemostatic effect.

The invention relates to the field of medical application of biological hydrogel, in particular to dexamethasone loaded hydrogel delivered across tympanic membranes and a preparation method of the dexamethasone loaded hydrogel, the hydrogel takes gelatin Gel and dopamine modified hyaluronic acid HADA as base materials, a three-dimensional network structure is formed through enzymatic crosslinking of transglutaminase mTGase, dexamethasone sodium phosphate Dex is loaded, and the hydrogel can be used for preparing the dexamethasone loaded hydrogel. Furthermore, local trans-tympanic membrane middle ear drug delivery and release are realized; specifically, a solution A composed of Gel, HADA and Dex and a solution B composed of mTGase are evenly mixed according to the volume ratio of 4: 1 and dropwise added to the surface of the tympanic membrane through an external auditory canal, and finally Gel-HADA-Dex hydrogel is formed on the surface of the tympanic membrane. Local precise high-concentration drug delivery can be achieved, systemic drug toxicity is avoided, materials are safe, ear toxicity is avoided, meanwhile, adhesion performance is high, drug release is continuous, gradual self-degradation is achieved, manual taking out is not needed, and use convenience is remarkably improved.

The application discloses a modified gelatin sustained-release capsule and a preparation method and application thereof, and the preparation method of the sustained-release capsule is as follows: S1, calcium magnesium phosphate fertilizer powder is added into deionized water, and stirring is conducted until the water solution is uniform; S2, gelatin particles are mixed with the water solution, and the gelatin particles are absorbed and swelled into gelatin gel; S3, glycerol is added into the gelatin gel, and the gelatin liquid is obtained by stirring and heating; S4, the gelatin liquid is prepared into a capsule film body, the capsule film body is immersed into a crosslinking agent solution for crosslinking; and S5, the crosslinked capsule film body is taken out, and the gelatin capsule is obtained after water drying. The gelatin, water, glycerol and calcium magnesium phosphate fertilizer are uniformly mixed into a solution by heating, and the solution is crosslinked into a shell by glutaraldehyde, and the crosslinking and the filling of the calcium magnesium phosphate fertilizer significantly enhance the mechanical property and the hydrophobic property of the gelatin, and better sustained-release effect is obtained. The fertilizer is packaged into the sustained-release capsule, so that the utilization rate of the fertilizer is improved, and the environment is not polluted.

The present disclosure provides a preparation method of a reverse osmosis composite membrane, including the following steps: coating a gelatin solution on a non-woven fabric layer and cooling to form a gelatin gel layer; coating a salt solution on a surface of the gelatin gel layer to obtain a gel / non-woven fabric composite membrane; placing the gel / non-woven fabric composite membrane in an aqueous phase solution, transferring into an oil phase solution, and conducting interfacial polymerization to obtain a non-woven fabric / gel / polyamide composite membrane; placing the non-woven fabric / gel / polyamide composite membrane in water, and heating to remove the gelatin gel layer to obtain a non-woven fabric / polyamide composite membrane; and introducing a trimesoyl chloride (TMC) gas from a side where the non-woven fabric layer is away from a polyamide layer in the non-woven fabric / polyamide composite membrane, conducting gas-liquid interfacial polymerization by chemical vapor deposition (CVD), and drying to obtain the reverse osmosis composite membrane.

The invention relates to a ZCDG nanoparticle-loaded chitosan and gelatin antibacterial antioxidant composite packaging film as well as a preparation method and application thereof, and belongs to the technical field of novel bio-based polymer composite materials. The preparation method of the composite packaging film comprises the following steps: (1) weighing chitosan CS, dissolving in an acetic acid-deionized water mixed solution, adding a plasticizer, and uniformly stirring to obtain a solution A; (2) weighing gelatin Gel, dissolving in deionized water, heating and stirring to obtain a solution B; (3) mixing the solution A and the solution B, heating and stirring to obtain a solution C; (4) dissolving ZCDG nanoparticles in water to obtain a solution D; and (5) adding the solution D into the solution C, ultrasonically stirring uniformly, pouring into a mold, and drying. The composite packaging film has excellent performances of good flexibility, oxidation resistance, antibacterial activity, thermal stability, biocompatibility, environmental protection, degradability and the like.

This invention relates to a myocardial repair patch based on multi-material printing, its preparation, and its application, belonging to the field of bio-3D printing and regenerative medicine technology. The invention uses PLCL and GelMA hydrogel loaded with active cardiomyocytes as bio-inks, and introduces gelatin gel as a heat buffer material. An orthogonal network structure is constructed using multi-material bio-3D printing technology to prepare a myocardial repair patch with excellent dynamic adaptability to the myocardial interface and good biocompatibility. The myocardial injury repair patch prepared by this invention achieves a structure-function integrated design through the synergistic effect of the three materials, significantly overcoming the limitations of single materials in terms of mechanical properties, biological function, or thermal compatibility. The mechanical properties of the myocardial injury repair patch prepared by this invention can precisely match those of natural myocardium, while possessing high cell activity, effectively inhibiting fibrosis, improving myocardial function, and achieving true active repair. Furthermore, the process conditions are controllable, demonstrating good translational potential.

The invention is directed to a method for reducing the endotoxin content of a gelatin, comprising the steps of providing a gelatin gel comprising the gelatin and endotoxins; followed by contacting the gelatin gel with an extraction solvent having a temperature of at most 10 °C, allowing at least a part of the endotoxins to dissolve in the extraction solvent; followed by separating at least part of the extraction solvent with dissolved endotoxins from the gelatin gel to obtain a purified gelatin gel comprising a purified gelatin; followed by contacting the purified gelatin gel with water causing the purified gelatin gel to swell; optionally followed by repeating steps b) to d) to further purify the purified gelatin gel.

The invention relates to a rapid self-condensing hydrogel dressing applied to wound repair and a preparation method of the rapid self-condensing hydrogel dressing. The dressing is prepared from phenylboronic acid grafted oxidized dextran POD, carbonyl dihydrazine modified gelatin Gel-CDH and nanoparticles PAC NPs self-assembled by procyanidine and chlorhexidine acetate. When the dressing disclosed by the invention is in contact with wound exudate, the dressing can be quickly self-crosslinked to form stable hydrogel, and has excellent tissue adhesiveness, self-healing capability and mechanical properties similar to skin. Besides, the hydrogel has broad-spectrum antibacterial activity, strong ROS (reactive oxygen species) removal capacity and high biocompatibility, can promote macrophages to be polarized to M2 phenotype, down-regulate proinflammatory cytokines and enhance anti-inflammatory markers, and has a good market application prospect.

The invention is directed to a method for purifying a modified gelatin, comprising the steps of providing a gelatin gel comprising the modified gelatin and contaminants; followed by contacting the gelatin gel with an extraction solvent having a temperature of at most 10 °C, allowing at least a part of the contaminants to dissolve in the extraction solvent; followed by separating at least part of the extraction solvent with dissolved contaminants from the gelatin gel to obtain a purified gelatin gel comprising a purified modified gelatin; followed by contacting the purified gelatin gel with water causing the purified gelatin gel to swell; optionally followed by repeating steps b) to d) to further purify the purified gelatin gel. The invention is further directed to products obtainable by the method, which products have an amount of contaminants of at most 200 ppm.

This invention belongs to the field of hydrogel material preparation technology, specifically relating to the preparation and application of an easily dispersible water-retaining agent. The water-retaining agent can be prepared through a simple operation. It is composed of gelatin and vanillin. Due to the grafting effect of vanillin and gelatin, this invention significantly prolongs the solidification time of the gelatin solution, giving the water-retaining agent good fluidity for a longer period, allowing it to be mixed into the application system via injection. Furthermore, through the cross-linking action of transglutaminase, it catalyzes the covalent cross-linking of gelatin chains, making it different from other gelatin gels; it does not melt upon heating after cooling and solidification, exhibiting thermal stability. This water-retaining agent, with its easily dispersible and thermally irreversible properties, has advantages in the mechanical mixing of application systems, which is beneficial for improving the structure of applied products and is expected to be widely used in food pre-processing, biomedical materials, and other fields.

The present invention relates to a nano Fe9S 10 @C A method for preparing a negative electrode composite material for a lithium-ion battery comprises the following steps: adding gelatin to deionized water, heating the mixture in a water bath while stirring, and turning the solution into a light yellow transparent gel solution; then adding FeSO4·7H2O, stirring the mixture at 60‑80°C for 8‑12 minutes, cooling the mixture naturally, forming a solid colloidal substance, and drying the mixture; heating the mixture in a tubular furnace and maintaining a constant temperature, and then cooling the mixture naturally to obtain the finished product. The advantages are: using a one-step method, the active material is uniformly coated during the high-temperature carbonization process to form a carbon-coated nanocomposite material. This method uses inexpensive and readily available gelatin as the base carbon, and FeSO4·7H2O as both the iron source and the sulfur source, which are dissolved in the gelatin gel solution, cooled, and further carbonized to form Fe9S with good conductivity. 10 @C nanocomposite materials.

The application provides a hemostatic material and a preparation method thereof. The preparation raw material of the hemostatic material comprises gelatin and a natural polymer material, and the preparation process of the hemostatic material comprises the step of crushing gelatin gel in the presence of the natural polymer material; wherein the gelatin gel is obtained by gelation after cross-linking of the gelatin. The hemostatic material of the application comprises gelatin and a natural polymer material, and the gelatin gel is crushed in the presence of the natural polymer material, so that the particle size of the gel can be reduced, and finally the hemostatic material with good hemostatic effect is obtained.