Plant-based cultured meat prepared based on vegetable protein scaffold and preparation method of plant-based cultured meat
A technology on plants and scaffolds, applied in the field of stem cell and animal cell cultured meat, can solve the problems of inapplicability to large-scale production of cultured meat, and achieve the effect of improving sensory quality, high nutritional value, and good meat quality
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Embodiment 1
[0106] The research of the screening of embodiment 1 food-grade plant drawing protein support
[0107] (1) Different commercialized plant-drawn protein pre-treatments.
[0108] This embodiment is divided into the scaffold group of peanut silk protein (manufacturer: Xiaobingsu, type: filament type), soybean silk protein (manufacturer: Xiaobingsu, type: filament type) scaffold group, wheat silk protein (manufacturer : There are many small soldiers, type: filament type) bracket group.
[0109] At room temperature, the three sets of drawn protein scaffolds were soaked in ultrapure water until they swelled completely. Wheat drawn protein and soybean drawn protein were soaked for 40-60 minutes to fully expand, and peanut protein was soaked for 5-10 minutes to fully expand. Then drain the water, and use fiber-free paper to suck the plant drawn protein to nearly dry, and observe it. It can be seen that the pores of wheat drawn protein are the largest after rehydration, followed by pe...
Embodiment 2
[0124] Example 2 The Research of Cell Adhesion and Growth State on Plant Protein Support
[0125] (1) The survival rate of cells in plant protein.
[0126] Example 1 (3) The peanut protein scaffolds inoculated with cells were placed in an incubator at 37°C, replaced with a differentiation medium for cultivation, the medium was replaced every two days, and cultured in the proliferation medium for about 6 days, the cells migrated to Evenly distributed in the scaffold, replace with myogenic cell differentiation medium. The differentiation medium described in the embodiment of the present invention includes 97vol% basal medium, 2vol% fetal bovine serum, and 1vol% penicillin-streptomycin double antibody solution; the basal medium is selected from DMEM / F12 medium; the penicillin -In the streptomycin double antibody solution, the content of penicillin is 10000U / ml, and the content of streptomycin is 10mg / ml. After 4 days of differentiation, samples were collected after culturing, a...
Embodiment 3
[0135] Example 3 Quality Research of Vegetable Protein Scaffold Inoculated with Smooth Muscle Cells
[0136] (1) Protein electrophoresis of plant-based cultured meat.
[0137] Process according to the method described in Example 1, wherein the "inoculation" described in the embodiment step (3) presses 5*10 6 Cells / scaffold, the plant-based cultured meat was obtained after differentiation was completed, and the blank peanut-drawn protein scaffold was used as a control, and 100 μL of RIPA (plus a final concentration of 1 mM PMSF) was added, and the tissue was broken by a freezer and then lysed on ice for 30 minutes to collect Store at -20°C until use. Then centrifuge at 12000g for 5 minutes, collect the supernatant, use Thermo Fisher’s BCA kit to measure and protein concentration, add 5X loading buffer according to 4:1 (V:V), mix and heat at 95°C for 5 minutes to denature the protein, -80 Store at ℃. Prepare SDS-page gel electrophoresis buffer. When the electrophoresis buffer...
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