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Cultured meat composition

A composition and cell technology, applied in the field of cultured meat composition, can solve problems such as unsustainable meat production system

Pending Publication Date: 2020-05-05
ALEPH FARMS LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Furthermore, the world's demand for meat is expected to double by 2050, meaning that traditional meat production systems are not sustainable

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0177] Three-dimensional structure analysis of TSP porous scaffold

[0178] The TSP scaffolds were examined using scanning electron microscopy (SEM) to analyze their three-dimensional (3D) structure on both the micro- and nanoscale (scale). The 100× image shows that TSP is porous with a pore size of 100–1,000 μm, which can be used for cell culture. showed thicker walls in large TSPs compared to TSPs of medium origin ( Figure 3A and 3B ). SEM image of large TSP (2×10 5 Magnification) shows that TSP contains 30 nm globular protein clusters. Analysis of medium-sized TSPs revealed more amorphous colloidal material between these clusters ( Figure 4A and 4B ).

Embodiment 2

[0180] Growth and Proliferation of Fibroblasts Cultured on Porous Scaffolds

[0181] Attachment and proliferation of fibroblasts on TSP scaffolds were examined. First, 50,000 skin fibroblasts labeled with RFP were seeded on TSP scaffolds and cultured. After 14 days of incubation, the TSP scaffolds filled with skin fibroblasts were reseeded with 200,000 endothelial cells and incubated for an additional 7 days. At days 8, 18, and 21 after fibroblast seeding, TSP samples filled with cells were immunostained and imaged using a confocal microscope. The results showed that even when seeded at a low cell number of 50,000 cells, fibroblasts proliferated and covered the TSP scaffold ( Figure 5A ). After seeding endothelial cells ( Figure 5B ), endothelial cells aggregated into clusters ( Figure 5C ). Next, examine scaffolds from each scaffold source and scaffolds cut from 90° relative to the surface orientation of the TSP. Each scaffold was seeded with 200,000 fibroblasts and...

Embodiment 3

[0183] Growth of Myoblasts Cultured on Porous Scaffolds

[0184] To assess the utility of generating muscle tissue, experiments were performed to investigate whether muscle cells could grow on TSP scaffolds.

[0185] For this purpose, the 0.5×10 6 Myoblasts were seeded on TSP scaffolds and cultured in the presence of a low-serum differentiation medium, making them more suitable for cultured meat production. Myoblast images on day 14 ( Figure 7 ) showed that myoblasts proliferated on the TSP scaffold, indicating that the cells could fill the scaffold after 14 days in culture.

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Abstract

The invention is directed to a method for producing an edible composition, comprising incubating a three-dimensional porous scaffold and a plurality of cell types comprising: myoblasts or progenitor cells thereof, at least one type of extracellular (ECM)-secreting cell and endothelial cells or progenitor cells thereof, and inducing myoblasts differentiation into myotubes.

Description

[0001] cross reference [0002] This application claims the benefit of priority to U.S. Provisional Patent Application No. 62 / 532,998, filed July 15, 2017, the contents of which are incorporated herein by reference in their entirety. technical field [0003] In particular the invention relates to cultured meat compositions and methods for their production. Background technique [0004] Cultured meat, also known as engineered or clean meat, is produced from cell cultures using tissue engineering techniques and is a prominent alternative to using live animals to produce traditional meat. Over the past decade, the concept has garnered increasing attention in public opinion, popular media, investors, and the scientific community, particularly after the production of the first lab-grown cultured beef burger. The production of food from animals is considered inefficient because animals consume large amounts of food during their lifetime, with 80-90% of the calories wasted in the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/07A61L27/56A23L13/00
CPCA23L13/00C12N5/0062C12N5/0659C12N5/0656C12N5/069C12N5/0691C12N2501/115C12N2501/105C12N2501/11C12N2533/50C12N2502/1335C12N2502/1347C12N5/0068C12N5/0689C12N5/0658
Inventor T·本阿里S·莱文伯格
Owner ALEPH FARMS LTD
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