Application of rare earth 1-hydroxyethylidene-1,1-diphosphonic acid tetrasodium salt
A technology of hydroxyethylidene bisphosphonate and hydroxyethylidene bisphosphonate, applied in the field of medicine, can solve the problems of high price, inability to be widely used, increased risk of endometrial cancer and ovarian cancer, etc., and has broad application prospects. Effect
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[0017] 1. In vitro test
[0018] Rare earth hydroxyethylidene diphosphonate (lanthanum hydroxyethylidene diphosphonate, cerium hydroxyethylidene diphosphonate, praseodymium hydroxyethylidene diphosphonate, neodymium hydroxyethylidene diphosphonate, promethium hydroxyethylidene diphosphonate, Samarium hydroxyethylidene diphosphonate, europium hydroxyethylidene diphosphonate, and gadolinium hydroxyethylidene diphosphonate) were prepared in PBS (phosphate buffer saline) to prepare 10 mg / mL stock solutions, and diluted to 1000 μg / mL before use. 100 μg / mL.
[0019] Experimental procedure: Take the primary cultured rat osteoblasts (OB) in 2×10 4 cells / mL were inoculated in a 96-well plate with a volume of 90 μL per well in CO 2 Cultivate in the incubator for 24h. After the cells adhere to the wall, add 10 μL of PBS solution of rare earth hydroxyethylidene bisphosphonate to make the final concentration of rare earth hydroxyethylidene bisphosphonate 100 μg / mL, 10 μg / mL, and after ...
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