Three-dimensional porous sponge scaffold with meniscus matrix source and preparation method and application

A three-dimensional porous, meniscus technology, applied in the field of tissue engineering scaffolds, the preparation of tissue engineering scaffolds, and the field of scaffold products, can solve the problem that it is difficult to fully meet the special requirements of meniscus regeneration, affects the microenvironment of the damaged repair site, and is unfavorable for tissue reconstruction. Regeneration and other issues, to achieve good clinical application prospects, suitable degradation rate, simple and easy preparation process.

Inactive Publication Date: 2016-08-31
GENERAL HOSPITAL OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantages of artificial synthetic materials are: hydrophobicity, which is not conducive to cell adhesion, the product is acidic when degraded, and it is easy to cause inflammation in the body, etc.
However, the collagen fibers and components of the material are quite different from those of the natural meniscus, and the degradation of the material will affect the microenvironment of the damaged repair site, which is not conducive to tissue reconstruction a...

Method used

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  • Three-dimensional porous sponge scaffold with meniscus matrix source and preparation method and application
  • Three-dimensional porous sponge scaffold with meniscus matrix source and preparation method and application
  • Three-dimensional porous sponge scaffold with meniscus matrix source and preparation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0035] 1. Preparation of three-dimensional porous sponge-like scaffold derived from meniscus matrix:

[0036] 1. Take the meniscus of the pig, peel off the fat and synovial tissue under sterile conditions, cut the remaining tissue into pieces (5mm×5mm×5mm), wash it with distilled water for 3 times, then soak it in three-distilled water at ‐20°C, hypotonic Freeze and thaw 5 times, 5 hours each time, remove the triple distilled water after thawing, soak and stir with hydrogen peroxide for half an hour, and then rinse with distilled water 3 times, the volume ratio of hydrogen peroxide to tissue is 1:1, and the treatment time is 0.5 hours. It is to remove antigens such as red blood cells, make the tissue white and bulky, and help the cells in the tissue break down and prolapse.

[0037] 2. Add acetic acid and distilled water to the tissue, use the pH value of the solution (adjust the pH value to 1‐3) to control the ratio of acetic acid and distilled water, and then add 1% (weight ...

Embodiment 2

[0051] 1. Take the meniscus of the cow, peel off the fat and synovial tissue under aseptic conditions, cut the remaining tissue into pieces (5mm×5mm×5mm), rinse with distilled water for 3 times, then soak in three-distilled water at -10°C, hypotonic Freeze and thaw 4 times, 6 hours each time. After thawing, remove the triple distilled water, soak and stir in hydrogen peroxide for 1 hour, and then rinse with distilled water 3 times. The volume ratio of hydrogen peroxide to tissue is 1:2, and the treatment time is 1 hour.

[0052] 2. Add acetic acid and distilled water to the tissue, use the pH value of the solution (adjust the pH value to 1‐3) to control the ratio of acetic acid and distilled water, and then add 3% (weight percent) pepsin with a volume ratio of 2:1 solution, then pour it into a pulverizer, and pulverize it in a low-temperature chromatography cabinet for 10 minutes to make a homogenate. The time for each pulverization should not exceed one minute, and each pulver...

Embodiment 3

[0059] 1. Take the meniscus of the sheep, peel off the fat and synovial tissue under sterile conditions, cut the remaining tissue into pieces (5mm×5mm×5mm), wash it with distilled water for 3 times, then soak it in three-distilled water at -40°C, hypotonic Freeze and thaw 3 times, 4 hours each time. After thawing, remove the triple distilled water, soak and stir in hydrogen peroxide for half an hour, and then rinse with distilled water 3 times. The volume ratio of hydrogen peroxide to tissue is 1:3, and the treatment time is 45 minutes.

[0060] 2. Add acetic acid and distilled water to the tissue, use the pH value of the solution (adjust the pH value to 1‐3) to control the ratio of acetic acid and distilled water, and then add 5% (weight percent) pepsin with a volume ratio of 2:1 solution, then pour it into a pulverizer, and pulverize it in a low-temperature chromatography cabinet for 15 minutes to make a homogenate. The time for each pulverization should not exceed one minute...

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Abstract

The invention discloses a three-dimensional porous sponge scaffold with a meniscus matrix source and a preparation method and an application. The method comprises the following steps: with meniscus as a raw material, removing cells through mechanical crushing treatment, acetic acid enzyme digestion and a differential centrifugation method to obtain meniscus extracellular matrix slurry; building a three-dimensional porous scaffold through freeze-drying; and carrying out crosslinked reinforcement on the scaffold through ultraviolet and chemical methods. The prepared three-dimensional porous sponge scaffold with the meniscus matrix source has a controllable microstructure, appropriate degradation rate, good biocompatibility and certain biomechanical strength, is beneficial to cell adhesion, uniform distribution of seed cells to enter into the scaffold and proliferation migration growth, is free of immunological rejection when implanted in vivo, can be widely applied to the field of meniscus and cartilage tissue engineering, and has a good clinical application prospect.

Description

technical field [0001] The invention belongs to the technical field of medical tissue engineering, and relates to a scaffold for tissue engineering, in particular to a method for preparing a scaffold for tissue engineering that promotes meniscal fibrocartilage and articular cartilage regeneration and a scaffold product prepared by the method. Background technique [0002] The meniscus is a kind of fibrocartilage tissue located between the femur and the tibia of the knee joint. Once the meniscus is damaged, especially in the inner region, due to its very limited self-repair ability, the treatment method is relatively monotonous, and a total or partial meniscus resection has to be performed. However, after meniscectomy, the knee meniscus completely or partially loses the biomechanical function of the normal meniscus, which will cause articular cartilage degeneration in the long run and lead to severe osteoarthritis. Allogeneic meniscus transplantation can replace the missing ...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/56A61L27/50
CPCA61L27/3612A61L27/3654A61L27/3683A61L27/50A61L27/56A61L2430/06
Inventor 郭全义苑志国刘舒云黄靖香郭维民高爽陈明学王明杰张雨
Owner GENERAL HOSPITAL OF PLA
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