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Tissue engineering meniscus scaffold and preparation method thereof

A tissue engineering, meniscus technology, applied in the direction of tissue regeneration, additive processing, medical science, etc., can solve problems such as the inability to meet the performance requirements of the meniscus stent, and achieve improved tensile and compressive elastic modulus, good mechanical properties, The effect of improving the survival rate

Active Publication Date: 2016-04-13
杭州弘新生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is to provide an FDM-based tissue engineering meniscus support and a preparation method for the problem that the existing FDM printing method for tissue engineering support cannot meet the performance requirements of the meniscus support

Method used

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  • Tissue engineering meniscus scaffold and preparation method thereof
  • Tissue engineering meniscus scaffold and preparation method thereof
  • Tissue engineering meniscus scaffold and preparation method thereof

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preparation example Construction

[0030] see figure 1 , is a flowchart of a method for preparing a tissue engineered meniscus scaffold according to a preferred embodiment of the present invention. Such as figure 1 As shown, the preparation method provided by this embodiment mainly includes the following steps:

[0031] First, in step S1, put the polycaprolactone (PCL) material into the nozzle of the fused deposition modeling (FDM) three-dimensional printer, heat it to 120-140°C, and prepare for printing under the pressure of 600-1000kPa. The fused deposition modeling 3D printer includes a high-precision pneumatic 3D experimental platform and a sharp pen direct writing nozzle. Preferably, the weight average molecular weight Mw of the PCL material is 60000, and the melting point is 60°C.

[0032] Subsequently, in step S2, the printing speed of the FDM three-dimensional printer is set to 0.6-0.75 mm / s, the fiber diameter is set to 300-320 μm, and the fiber spacing is set to 200-300 μm, and the tissue engineeri...

Embodiment 1

[0040] 1. Put the polycaprolactone material into the nozzle of the fused deposition modeling 3D printer, heat it to 120-140°C, and prepare for printing under the pressure of 600-1000kPa.

[0041] 2. Set the printing speed of the fused deposition modeling 3D printer to 0.6-0.75 mm / s, the fiber diameter to 300 μm, and the fiber spacing to 200 μm to print out a ring-shaped tissue engineering meniscus scaffold, in which the ring outer diameter d1 is 10 mm. The inner diameter d2 of the ring is 4 mm, and the thickness h is 1.5 mm.

[0042] 3. Using cobalt-60 to sterilize the tissue engineered meniscus bracket by irradiation.

Embodiment 2

[0044] 1. Put the polycaprolactone material into the nozzle of the fused deposition modeling 3D printer, heat it to 120-140°C, and prepare for printing under the pressure of 600-1000kPa.

[0045] 2. Set the printing speed of the fused deposition modeling 3D printer to 0.6-0.75 mm / s, the fiber diameter to 320 μm, and the fiber spacing to 300 μm to print out a ring-shaped tissue engineering meniscus scaffold, in which the ring outer diameter d1 is 10.5 mm , the ring inner diameter d2 is 4.5mm, and the thickness h is 1.6mm.

[0046] 3. Using cobalt-60 to sterilize the tissue engineered meniscus bracket by irradiation.

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Abstract

The invention provides a tissue engineering meniscus scaffold and a preparation method thereof. The preparation method comprises the following steps: placing a polycaprolactone material into a nozzle of a fused deposition modeling three-dimensional printer to be heated to 120-140 DEG C, and preparing for printing at the air pressure of 600-1000 kPa; setting the printing speed of the fused deposition modeling three-dimensional printer to be 0.6-0.75 mm / s, the fiber diameter to be 300-320 [mu]m and the fiber interval to be 200-300 [mu]m and printing a tissue engineering meniscus scaffold; using cobalt-60 for irradiation and sterilization treatment. As the fused deposition modeling method is adopted to print the tissue engineering meniscus scaffold with an appropriate pore structure, an appropriate fiber diameter and appropriate biomechanical strength, the survival rate and the reproduction rate of inoculated cells and autologous in-growth cells of implanted regions are favorably increased, the fibrous cartilage differentiation rate can be increased, and newly generated meniscus tissues have favorable functions.

Description

technical field [0001] The invention relates to the technical field of biological materials and tissue engineering, and more specifically relates to a tissue engineering meniscus bracket and a preparation method thereof. Background technique [0002] The meniscus is composed of fibrocartilage, one on the inside and one on the inside. It is located in the tibiofemoral joint space of the knee joint and is an important part of the knee joint. It has important functions such as absorbing shock, transmitting load, nourishing articular cartilage and maintaining joint stability. Severe damage or removal of the meniscus can accelerate the development of knee osteoarthritis. Although the short-term curative effect after meniscectomy is good and the function recovery is fast, the regenerated meniscus does not have the function of a normal meniscus, and the long-term degeneration of articular cartilage will be aggravated. Advances in tissue engineering technology provide a new treatm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/18A61L27/38B33Y10/00
CPCA61L27/18A61L27/3834A61L27/3852A61L2430/06C08L67/04
Inventor 余家阔张正政江东张磊丁建勋
Owner 杭州弘新生物科技有限公司
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