Naringin microsphere silk fibroin/hydroxyapatite composite scaffold and preparation method thereof

A hydroxyapatite and composite scaffold technology, applied in the field of tissue engineering, can solve the problems of inability to be degraded and absorbed, poor biocompatibility, no bone formation, etc., so as to facilitate large-scale popularization and application, promote bone formation, and improve bone formation. The effect of microstructure

Inactive Publication Date: 2018-06-15
THE FIRST AFFILIATED HOSPITAL OF GUANGZHOU UNIV OF CHINESE MEDICINE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Polymethyl methacrylate is a commonly used implant material. Although it can provide sufficient support strength and cementation, it also has some obvious disadvantages: it cannot be degraded and absorbed in the body, has poor biocompatibility, has no biological activity, and has no biological activity when polymerized. A large amount of heat is released, and unpolymerized monomers are to

Method used

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  • Naringin microsphere silk fibroin/hydroxyapatite composite scaffold and preparation method thereof
  • Naringin microsphere silk fibroin/hydroxyapatite composite scaffold and preparation method thereof
  • Naringin microsphere silk fibroin/hydroxyapatite composite scaffold and preparation method thereof

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preparation example Construction

[0031] The preparation method of the drynaria-loaded microglobe silk fibroin / hydroxyapatite composite scaffold of the present invention comprises the following steps:

[0032] (1) Preparation of silk fibroin solution

[0033] Cut the silkworm cocoons into pieces, place them in a sodium bicarbonate solution with a concentration of 0.5 wt%, heat and boil for 0.5-2 hours, then wash with deionized water to remove colloids, and repeat this step three times. The silk fibroin from which the gum has been removed is placed in an oven at 50° C. to dry for 24 hours to remove the moisture in the silk fibroin.

[0034] Cut the dried silk fibroin into pieces, and then place it in a lithium bromide solution with a concentration of 9.3mol / L, wherein the ratio of silk fibroin to lithium bromide solution is (1-3):10(m:v), 40- Heat in a water bath at 60°C for 5-8h. After the silk fibroin is completely dissolved in the lithium bromide solution, the resulting mixed solution is placed in a dialys...

Embodiment 1

[0044] The preparation method of the drynaria-loaded microglobe silk fibroin / hydroxyapatite composite scaffold of this embodiment comprises the following steps:

[0045] (1) Preparation of silk fibroin solution

[0046] After the silkworm cocoons were cut into pieces, they were placed in a sodium bicarbonate solution with a concentration of 0.5wt%, heated and boiled for 0.5h, and then washed with deionized water to remove colloids. This step was repeated three times. The silk fibroin from which the gum has been removed is placed in an oven at 50° C. to dry for 24 hours to remove the moisture in the silk fibroin.

[0047] Cut the dried silk fibroin into pieces, then place it in a lithium bromide solution with a concentration of 9.3 mol / L, wherein the ratio of silk fibroin to lithium bromide solution is 1:10 (m:v), and heat in a water bath at 60°C for 5 hours. After the silk fibroin is completely dissolved in the lithium bromide solution, the resulting mixed solution is placed ...

Embodiment 2

[0057] The preparation method of the drynaria-loaded microglobe silk fibroin / hydroxyapatite composite scaffold of this embodiment comprises the following steps:

[0058] (1) Preparation of silk fibroin solution

[0059] After the silkworm cocoons were cut into pieces, they were placed in a sodium bicarbonate solution with a concentration of 0.5 wt%, heated and boiled for 1 hour, then washed with deionized water to remove colloids, and this step was repeated three times. The silk fibroin from which the gum has been removed is placed in an oven at 50° C. to dry for 24 hours to remove the moisture in the silk fibroin.

[0060] Cut the dried silk fibroin into pieces, then place it in a lithium bromide solution with a concentration of 9.3mol / L, wherein the ratio of silk fibroin to lithium bromide solution is 2:10 (m:v), and heat it in a water bath at 40°C for 6h. After the silk fibroin is completely dissolved in the lithium bromide solution, the resulting mixed solution is placed ...

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Abstract

The invention discloses a composite scaffold. The composite scaffold is prepared from naringin, gelatin microspheres, hydroxyapatite and silk fibroin. The composite scaffold is prepared by the following steps: 1) dropwise adding a 0.5-1g/ml naringin solution into the gelatin microspheres, then standing at the temperature of 2 to 8 DEG C over night, and performing freeze drying to obtain a naringin/gelatin microsphere compound; 2) mixing the hydroxyapatite and the naringin/gelatin microsphere compound with a 3.5 to 10 percent by weight SF solution, injecting the mixed solution into a pore plate, then performing freeze drying on the sample to obtain an NG/GMs/HA/SF composite scaffold. The traditional SF/HAp composite scaffold is loaded with the traditional Chinese medicinal monomer, and encapsulated with gelatin microspheres conforming to a bone microenvironment, thereby promoting healing of OVF and enhancing the mechanical strength of the fractured vertebral body; the composite scaffoldhas high rheological property, plasticity, biomechanical strength and higher bone formation prompting performance, so that the composite scaffold can be applied to bone defect areas of different shapes, has a durable effect and higher bone tissue repair ability, and is convenient for large-scale popularization and application.

Description

technical field [0001] The invention relates to the technical field of tissue engineering, in particular to a naringin microglobe silk fibroin / hydroxyapatite composite scaffold and a preparation method thereof. Background technique [0002] Biomaterial scaffolds, as one of the three major elements of regenerative medicine, play an important role in the bone remodeling process, so they are often used in research to treat OVF. Among many biological materials, hydroxyapatite has attracted much attention in the research of bone defect regeneration due to its good osteoconductive properties and similar mineralization process and structure to bone tissue. Silk fibroin is a kind of natural biopolymer with high mechanical strength, controllable biodegradability, good biocompatibility and easy handling. Using modern science and technology to make silk fibroin and hydroxyapatite into a composite scaffold can not only integrate the advantages of the two, but also avoid the shortcoming...

Claims

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Application Information

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IPC IPC(8): A61L27/46A61L27/50A61L27/54
CPCA61L27/46A61L27/50A61L27/54A61L2300/23A61L2300/412A61L2300/622A61L2430/02C08L89/00
Inventor 江晓兵梁德任辉余翔沈耿杨郭瑞
Owner THE FIRST AFFILIATED HOSPITAL OF GUANGZHOU UNIV OF CHINESE MEDICINE
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