The invention belongs to the technical field of animal cell culture, and relates to a cardiac muscle ring culture method, which comprises: pre-treating a heart sample, and preparing small ventricularmuscle tissue blocks; digesting the small ventricular muscle tissue blocks, and terminating; collecting the supernatant after the terminating, and culturing to prepare an initial-stage cardiac musclering cell liquid; and carrying out cell liquid exchange on the initial-stage cardiac muscle ring cell liquid, and observing the dynamic growth of the cardiac muscle ring. According to the present invention, the cardiac muscle ring-like structure is found during the in vitro isolated culture of the cardiac muscle cells, and is initially named cardiac muscle ring, wherein the detailed research on the cell components, the forming mechanism and the differentiation potential of the cardiac muscle ring is necessary; and the representation of the structure can provide important significance for the further research on the cardiac muscle stem cell function and the molecular regulation mechanism thereof, the seed cell source enriching, the new approach for the research and development of cardiac muscle regeneration, and the like, can provide theoretical basis for the self-regeneration and differentiation regulation of cardiac stem cells, and can provide new ideas for the clinical treatment of ischemic heart disease.