The invention mainly relates to the field of plantation and discloses a culture medium for Dendrobium officinale. The culture medium for Dendrobium officinale is prepared from the following raw materials: leftovers of domestic fungi, pumpkin pith, shaddock peel, pine needles, cattail, Utricularia bifida, ceramsite, brewer's yeast, bitter apricot kernel extract and Chinese peashrub root extract. According to the invention, the raw materials are widely available; the prepared culture medium has balanced nutrients, is air-permeable, water-retaining and fertilizer-retaining and enables the survival rate of cultured Dendrobium officinale to reach 100%, output to be increased by 16.8% and economic income to be increased by 14.3%; through refrigeration, stir-frying and fermentation of the medium, nutrients and active ingredients can be effectively extracted, and the medium can provide abundant raw materials needed in growth of Dendrobium officinale, is capable of preventing bacteria, killing insects and inhibiting generation of plant diseases and insect pests, reduces and even avoids usage of pesticides, protects Dendrobium officinale from pesticide residues and improves the grade of Dendrobium officinale; and a plurality of plant extraction agents are added into the medium, so growth of Dendrobium officinale is benefited, the harvesting period of Dendrobium officinale is 20 to 25 d earlier compared with the prior art, and economic income is increased.