Composition based on stem cells and application thereof in preparing preparation for coronary heart disease
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A technology of stem cells and compositions, applied in the biological field, to achieve the effects of regulating heart function, limiting expansion, and promoting blood vessel regeneration
Inactive Publication Date: 2015-09-23
奥思达干细胞有限公司
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At present, there is no ideal seed cell to meet the clinical needs of cell biology therapy for these cardiovascular diseases, especially coronary heart disease patients. There are many technical bottlenecks that need to be broken through, and further exploration and research are needed. The following issues need to be clarified: (1) The best type of cell transplantation and its composition; (2) How to ensure that the transplanted stem cells are colonized, fused and further differentiated in the target area, so as to ensure the survival rate of stem cells after transplantation; (3) The specific function of stem cells after transplantation , whether to exert the maximum effect; (4) What is the optimal time window for transplantation and the amount of transplanted cells?
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specific Embodiment 1
[0040] Specific Example 1: Preparation of Stem Cell Composition
[0041] 1. Screening of stem cells:
[0042] The bone marrow-derived pluripotent stem cell suspension after expansion and adherent culture was collected, and its surface markers were detected by flow cytometry, and the expression results of PE-CD105, PE-CD73, PE-CD44 and PE-CD13 were detected respectively. 96.78%, 90.03%, 95.86% and 90.69% were positive expression; the expression results of FITC-CD34, FITC-CD45, FITC-HLA-ABC (HLA-I) and FITC-HLA-DR (HLA-II) were respectively It was 2.24%, 1.03%, 0.08% and 1.69%, which were positive expressions; the negative control results showed 1.63%, which met the selection criteria for the preferred preparation of stem cells.
[0043] The same detection method can also be used for pluripotent stem cells derived from human embryos, umbilical cords, cord blood, and placenta, and the results should be positive for the expression of surface markers CD105, CD73, CD44, and CD13; C...
specific Embodiment 2
[0048] Specific Example 2: Application of Stem Cell Composition in Coronary Heart Disease
[0049] 1. Preparation of rat myocardial infarction model and grouping
[0050] Wistar rats, male or female, with a body weight (BW, 300±30g), were used to establish an acute myocardial infarction model. Randomly divided into 5 groups: 10 rats in each group, respectively normal control group (normal group), infarction untreated group (infarction group), infarction transplantation experimental group (experimental group), infarction transplantation group A (group A), infarction transplantation group A (group A), infarction transplantation group Group B (group B).
[0051] 2. The method for implementing the application of the composition based on stem cells in the preparation of preparations for coronary heart disease is specifically:
[0052] (1) Prepare a composition based on stem cells, wherein each dose contains the number of stem cells that have undergone preferred preparation operat...
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Abstract
The invention discloses a composition based on stem cells and application thereof in preparing preparation for the coronary heart disease. The composition based on the stem cells includes the stem cells prepared through an optimizing method and cell preserving liquid serving as a solvent. Firstly, proper stem cells are selected; secondly, the selected stem cells are further processed through the optimizing method; lastly, the stem cells are collected and resuspended in the cell preserving liquid. A various cell implanting method is adopted in the application of the composition based on the stem cells in preparing the preparation for the coronary heart disease, the time nodes before an operation, during the operation and after the operation are combined, and it is ensured that the transplanted stem cells can give the full play. The optimizing method is used for preparing the stem cells and the stem cell composition in the cell preserving liquid, the ventricle function can be extremely obviously enhanced after cell transplantation, and the left ventricle reconstitution is restrained. The optimal cell composition is provided for treating the coronary heart disease, the source is wide, preparation is simple, toxic and side effects are avoided, and the transplanting effect is good.
Description
technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a stem cell-based composition and its application in preparing preparations for coronary heart disease. Background technique [0002] Cardiovascular disease is one of the major diseases that seriously threaten human life and health. Coronary heart disease is the abbreviation of coronary heart disease (coronary artery heart disease, CHD), which is the most common heart disease. disease, it is also called ischemic cardiomyopathy. The World Health Organization divides coronary heart disease into five categories: asymptomatic myocardial ischemia (occult coronary heart disease), angina pectoris, myocardial infarction, ischemic heart failure (ischemic heart disease) and sudden death. Clinically, it is often divided into stable coronary artery disease and acute coronary syndrome. [0003] Coronary heart disease is one of the diseases with the highest mortality rate in the wor...
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