Nerve film and preparation method thereof

A nerve and thin film technology, applied in the field of biomedicine, can solve the problems of cells not easy to adhere, cells not attractive, etc.

Active Publication Date: 2019-06-11
KUNMING MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The existing scaffold materials usually use polyurethane, which has degradability and good blood compati

Method used

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  • Nerve film and preparation method thereof
  • Nerve film and preparation method thereof
  • Nerve film and preparation method thereof

Examples

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preparation example Construction

[0025] A preparation method of a nerve film, comprising: mixing polycaprolactone and isocyanate substances to react at a temperature of 60-80°C, and then adding a chain extender to react to obtain a polyurethane prepolymer.

[0026] Polycaprolactone reacts with isocyanate substances to generate polyurethane prepolymer under the action of chain extender. Under the action of the chain extender, the molecular chain of the generated polyurethane prepolymer is extended and the molecular weight is increased, which improves the mechanical properties (for example, twisting and folding properties) of the polyurethane prepolymer. Optionally, the polycaprolactone has a molecular weight of 2000.

[0027] In some optional embodiments, the chain extender includes ethyl lysine dihydrochloride, 1,4-butanediol, m-phenylenediamine, 1,2-ethylenediamine ethyl lysine dihydrochloride salt, ethylenediamine, carbamate, DL-lactic acid, and ascorbic acid. Among them, ethylenediamine is non-toxic and ...

Embodiment 1

[0042] The present embodiment provides a nerve membrane, which is prepared by the following steps:

[0043] Add 30.0g of polycaprolactone (molecular weight: 2000) into a 150ml small beaker. After the polycaprolactone is completely dissolved, add 7.8g of isophorone diisocyanate, react at 70°C for 2 hours, and add the catalyst stannous octoate dropwise. 0.1mL, continue to react for 2 hours, add chain extender lysine ethyl ester dihydrochloride 3.7g, react for 2.5 hours to obtain medical polyurethane prepolymer.

[0044] Add 0.25g gastrodin to the medical polyurethane prepolymer, react for 3 hours to obtain the first primary product; add 0.2mL water to the first primary product and continue to react for 0.5 hour to obtain the second primary product; put the second primary product at 90°C Curing and drying in an oven for 18 hours to obtain a medical polyurethane nerve material.

[0045] Weigh 2.0g of the above-mentioned medical polyurethane nerve material, add 20mL of 1,4-dioxane...

Embodiment 2

[0048] The present embodiment provides a nerve membrane, which is prepared by the following steps:

[0049] Add 30.0g of polycaprolactone (molecular weight: 2000) into a 150ml small beaker. After the polycaprolactone is completely dissolved, add 7.8g of isophorone diisocyanate, react at 70°C for 2 hours, and add the catalyst stannous octoate dropwise. 0.1mL, continue to react for 2 hours, add chain extender lysine ethyl ester dihydrochloride 3.7g, react for 2.5 hours to obtain medical polyurethane prepolymer.

[0050] The medical polyurethane prepolymer and gastrodin were mixed according to the mass ratio of 100:1, and the first product was obtained in 2 hours after reaction; 0.2mL water was added to the first product and continued to react for 20min to obtain the second product; The primary product was cured and dried in an oven at 100°C for 20 hours to obtain a medical polyurethane nerve material.

[0051] Weigh 2.0 g of the above-mentioned medical polyurethane nerve materi...

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Abstract

The invention provides a nerve film and a preparation method thereof, and relates to the field of biomedicine. The preparation method of the nerve film comprises: mixing polycaprolactone with an isocyanate substance for a reaction, and then adding a chain extender for a reaction, so as to obtain a polyurethane prepolymer; mixing the polyurethane prepolymer with gastrodin for a reaction, so as to obtain a first preliminary product, mixing the first preliminary product with water for a reaction, so as to obtain a second preliminary product, curing the second preliminary product and conducting drying to obtain a polyurethane nerve material; dissolving the polyurethane nerve material by an organic solvent, reacting the dissolved polyurethane nerve material with a pore-forming agent to obtain an emulsion initial product, conducting performing and air drying on the emulsion initial product, and dissolving the pore-forming agent by water under a vacuum condition, so as to obtain the nerve film. The nerve film is a porous structure through which pores penetrate, and the porous structure is favorable for cell adhesion and infiltration. The nerve film has good tortuosity, folding propertiesand biocompatibility. Slowly released gastrodin of the material is beneficial to cell proliferation and up-regulation of neurotrophic factors, and promotes elongation of axons.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a nerve membrane and a preparation method thereof. Background technique [0002] Tissue engineering is to implant cells into a material scaffold that can be degraded and absorbed in the human body after being cultured and diffused in vitro, providing cells with a three-dimensional space for reproduction and regeneration, and then implanting the scaffold into the human body to achieve the purpose of treatment . [0003] The existing scaffold materials usually use polyurethane, which has degradability and good blood compatibility. However, polyurethane is not attractive to cells as a drug carrier, and cells are not easy to adhere to. Contents of the invention [0004] Embodiments of the present invention provide a nerve membrane and a preparation method thereof. The nerve membrane prepared by the preparation method has a porous structure, which is beneficial to the adhesion, climbing ...

Claims

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Application Information

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IPC IPC(8): C08J9/26C08L75/06C08G18/12C08G18/42C08G18/66C08G18/38C08G18/32A61L27/18A61L27/50A61L27/54A61L27/56
Inventor 李丽梅陆地李庆钟莲梅郑梦于玛丽
Owner KUNMING MEDICAL UNIVERSITY
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