Biodegradable nerve conduit with bilayer structure and preparation method thereof

A technology of nerve conduit and double-layer structure, which is applied in the field of biodegradable nerve conduit and its preparation, can solve problems such as the influence of mechanical properties, and achieve the effects of promoting regeneration and repair, high economic benefits, and high mechanical strength

Inactive Publication Date: 2011-06-08
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the nerve guide in his invention has no skeleton structure, and its mechanical properties will be affected

Method used

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  • Biodegradable nerve conduit with bilayer structure and preparation method thereof
  • Biodegradable nerve conduit with bilayer structure and preparation method thereof
  • Biodegradable nerve conduit with bilayer structure and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) The PGLA monofilament obtained by melt spinning is braided into a mesh circular tube with an inner diameter of 2 mm, which is inserted into the rotating receiving circular tube of the electrospinning machine device after being fixed-length and shaped, and the rotation speed is 4000 rpm;

[0028] (2) PLA is dissolved in tetrahydrofuran / DMF solution (tetrahydrofuran: the volume ratio of DMF=3: 1), after stirring, it is PLA / tetrahydrofuran / DMF solution with 6% PLA mass percentage to make; silk fibroin is dissolved in three In the fluoroacetic acid solution, the silk fibroin protein mass percentage is 10% spinning stock solution after stirring;

[0029] (3) Import the above two spinning stock solutions into two syringe pumps respectively. The two syringe pumps have two nozzles with the same axis inside and outside to carry out electrospinning. Adjust the flow rate of the propulsion pump to 1.3mL / h and apply high pressure. 10kv, the receiving distance is 18cm;

[0030] ...

Embodiment 2

[0032] (1) The PGLA monofilament obtained by melt spinning is braided into a mesh circular tube with an inner diameter of 2 mm, which is inserted into the rotating receiving circular tube of the electrospinning machine device after being fixed-length and shaped, and the rotation speed is 4000 rpm;

[0033] (2) Dissolve PBS / PCL (mass ratio 1:4) in chloroform solution, stir to make PBS / PCL / chloroform solution with solute mass fraction of 10%; dissolve gelatin in formic acid solution, stir to make Gelatin mass fraction is the spinning stock solution of 10%;

[0034] (3) Import the two kinds of spinning stock solutions into two syringe pumps respectively. The two syringe pumps have two nozzles with the same axis inside and outside to carry out electrospinning. The flow rate of the propulsion pump is adjusted to 1.3mL / h, and the high pressure is 7.53kv, the receiving distance is 18cm;

[0035] (4) After electrospinning, the nerve guide was removed, sterilized by cobalt 60 irradiat...

Embodiment 3

[0037] (1) The PGLA monofilament obtained by melt spinning is braided into a mesh circular tube with an inner diameter of 0.5mm, and after being fixed-length and shaped, it is inserted into the rotating receiving circular tube of the electrospinning machine device, and the rotation speed is 4000rpm;

[0038] (2) Dissolve PBS / PLA (mass ratio 1:4) in chloroform solution, stir to make PBS / PLA / chloroform solution with solute mass fraction of 10%; dissolve gelatin in formic acid solution, stir to make Gelatin mass fraction is the spinning stock solution of 10%;

[0039] (3) Import the two kinds of spinning stock solutions into two syringe pumps respectively. The two syringe pumps have two nozzles with the same axis inside and outside to carry out electrospinning. Adjust the flow rate of the propulsion pump to 1.3mL / h, and apply high pressure to 8kv, the receiving distance is 18cm;

[0040] (4) After the electrospinning, the nerve guide was removed, sterilized by irradiation with c...

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Abstract

The invention relates to a biodegradable nerve conduit with a bilayer structure and a preparation method thereof. The biodegradable nerve conduit comprises a reticular monofilament liner which is made of polyglycollide-lactide (PLGA) and used as a framework structure and a nano fiber felt which is made of a biodegradable material and used as an outer layer structure. The preparation method comprises the following steps of: (1) preparing PLGA monofilaments by adopting a fused spinning method, stretching for orientating, and then weaving into a reticular liner conduit; (2) dissolving the biodegradable material into an organic solvent to prepare a spinning stock solution with a mass fraction of 3-20 percent; and (3) carrying out electrostatic spinning by using the spinning stock solution on the surface of the reticular liner conduit by taking the reticular liner conduit as a receiving device to obtain the biodegradable nerve conduit. The biodegradable nerve conduit has good biocompatibility, proper degradation time and higher mechanical strength and can promote the regeneration and the restoration of damaged nerves. The preparation method has the advantages of simpleness of operation, low cost, environmental protection, high economic benefit and good application prospect.

Description

technical field [0001] The invention belongs to the field of biodegradable nerve conduits, in particular to a biodegradable nerve conduit with a double-layer structure and a preparation method thereof. Background technique [0002] At the end of the 19th century, Cluck first used bone-free calcium to make a bony tube to bridge the nerve defect and perform nerve repair, and research on the use of catheters to repair nerve defects continued. Including Weiss's concept of repairing nerve damage by using the method of sutureless catheterization proposed by Weiss in 1944, and the artificial nerve transplantation technology gradually developed in the late 1970s, that is, using biological materials to prepare nerve guides, and inside the guide tube Create a microenvironment for nerve growth in the cavity, guide and promote nerve regeneration. In recent years, with the continuous research and exploration of tissue engineering technology research, tissue engineered nerve transplantat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/50A61L27/40A61L27/22A61L27/18A61F2/04D01D5/00D01D1/02
Inventor 杨庆邵梅玲郯志清沈新元张佩华王文祖陈璐
Owner DONGHUA UNIV
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