Preparation method of nanofiber scaffold for heart tissue engineering
A nanofiber and cardiac tissue technology, applied in the field of preparation of nanofiber scaffolds, can solve the problems of limited fiber size, complex preparation process, and inability to precisely control the performance of scaffolds.
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[0012] In order to deepen the understanding of the present invention, the present invention will be further described below in conjunction with the examples.
[0013] A method for preparing a nanofibrous scaffold material with adjustable mechanical and structural properties used in the field of myocardial tissue engineering, the steps of which are as follows:
[0014] (1) Preparation of PGS (Mw=10000): Sebacic acid and glycerol were reacted at 160°C for 36h under nitrogen and high vacuum (<50mTorr) atmosphere at a molar ratio of 1:1 to synthesize PGS.
[0015] (2) PGS / gelatin nanofibrous scaffolds with different compositions were prepared by electrospinning: PGS and gelatin were mixed in different mass ratios (2:1, 1:2 and 0:1) at 80% (v / v ) in acetic acid solution to prepare 20% (w / v) spinning solution. The spinning liquid was injected into a 1mL syringe with a syringe pump for electrospinning. The spinning speed was 2ml / h, the spinning distance was 10cm, and the spinning vo...
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