Preparation method of nanofiber scaffold for heart tissue engineering

A nanofiber and cardiac tissue technology, applied in the field of preparation of nanofiber scaffolds, can solve the problems of limited fiber size, complex preparation process, and inability to precisely control the performance of scaffolds.

Inactive Publication Date: 2014-02-26
WUXI ZHONGKE GUANGYUAN BIOMATERIALS
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  • Summary
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Problems solved by technology

However, the preparation process of such scaffold materials is often very complicated, the performance of the scaffold cannot be precisely controlled, the porous structure is randomly formed, the size of the fibers is limited (micron scale), and it is easy to degrade

Method used

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Examples

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Embodiment Construction

[0012] In order to deepen the understanding of the present invention, the present invention will be further described below in conjunction with the examples.

[0013] A method for preparing a nanofibrous scaffold material with adjustable mechanical and structural properties used in the field of myocardial tissue engineering, the steps of which are as follows:

[0014] (1) Preparation of PGS (Mw=10000): Sebacic acid and glycerol were reacted at 160°C for 36h under nitrogen and high vacuum (<50mTorr) atmosphere at a molar ratio of 1:1 to synthesize PGS.

[0015] (2) PGS / gelatin nanofibrous scaffolds with different compositions were prepared by electrospinning: PGS and gelatin were mixed in different mass ratios (2:1, 1:2 and 0:1) at 80% (v / v ) in acetic acid solution to prepare 20% (w / v) spinning solution. The spinning liquid was injected into a 1mL syringe with a syringe pump for electrospinning. The spinning speed was 2ml / h, the spinning distance was 10cm, and the spinning vo...

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Abstract

The invention relates to a preparation method of a nanofiber scaffold for heart tissue engineering. The preparation method comprises the steps of preparing the nanofiber scaffold from poly(glyceroi sebacate) (PGS)/a gelatin nanofiber scaffold material with different components through electrostatic spinning, and crosslinking the nanofiber scaffold in a mixed solution of hydrochlorinated N,N-(3-dimethylaminopropyl)-N'-ethyl-carbodiimide (EDC) and N-hydroxysuccinimide (NHS) to obtain the nanofiber scaffold. The nanofiber scaffold related by the invention can be used for promoting the formation of sarcomere and has wide prospects on the aspect of being used as a material for cardiac muscle cell regeneration and repair.

Description

technical field [0001] The invention relates to the field of myocardial tissue repair and regeneration, in particular to the preparation of a nanofiber scaffold for cardiac tissue engineering. Background technique [0002] A very important challenge in the field of cardiac tissue engineering is the development of biomimetic grafting techniques that can potentially facilitate myocardial tissue repair and regeneration. Much research has focused on simulating the anisotropic structure of cardiomyocytes by constructing a series of biophysical environments. For example, electrospinning has been widely used as a simple but flexible method to fabricate high-porosity nanofibrous scaffolds with internal cross-linked pores to mimic the structure of cardiac muscle. Such nanofibrous scaffolds can induce cell alignment and enhance cell migration at intercellular junctions and ultimately induce the construction of vascular tissue. Some approaches use engineered scaffolds to mimic myocar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/18A61L27/20A61L27/58
Inventor 韩志超许杉杉
Owner WUXI ZHONGKE GUANGYUAN BIOMATERIALS
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