Preparation method for polylactic acid nanofiber scaffold functionally modified by two-dimensional nanometer black phosphorus

A nanofiber, modification and modification technology, which is applied in the preparation of polylactic acid nanofiber scaffolds and the preparation of bone repair biomaterials, can solve the problems of poor photothermal performance, unstable performance of black phosphorus, and easy oxidation, etc., to achieve biological Improve activity, achieve controlled release and long-acting effect, reduce agglomeration

Active Publication Date: 2020-03-27
福建渤海传芳企业发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, black phosphorus is unstable in performance. It is prone to oxidation under visible light and humid environment, and degrades to form phosphorus oxides and p

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1) Preparation of black phosphorus nanosheets: block black phosphorus was ground into powder and then added to N-methylpyrrolidone solvent to obtain black phosphorus N-methylpyrrolidone solution with a concentration of 1 mg / mL. After 8 hours of variable-frequency ultrasound (45 HZ, 80 HZ, 100 HZ) in an ice-water bath, the black phosphorus nanosheets were centrifuged at a speed of 10,000 rpm to obtain a precipitate of black phosphorus nanosheets, and the precipitate was dried to obtain black phosphorus nanosheets for use.

[0024] 2) Preparation of drug-loaded black phosphorus: Weigh 5 mg of the black phosphorus nanosheets prepared in step 1) and add them to the ibuprofen solution with a concentration of 18 mg / mL, absorb at constant temperature for 20 h, centrifuge and dry the precipitate , to obtain drug-loaded black phosphorus for use.

[0025]3) Preparation of drug-loaded black phosphorus / sodium alginate composite microspheres: add 100 g cyclohexane and 10 g dispersio...

Embodiment 2

[0028] 1) Preparation of black phosphorus nanosheets: block black phosphorus was ground into powder and added to N-methylpyrrolidone solvent (concentration: 1 mg / mL). After 10 hours of variable-frequency ultrasound (45HZ, 80HZ, 100HZ) in an ice-water bath, centrifuge at a speed of 10,000 rpm to obtain black phosphorus nanosheet precipitates, and dry the precipitates for later use.

[0029] 2) Preparation of drug-loaded black phosphorus: Weigh 5 mg of black phosphorus nanosheets and add them to the ibuprofen solution with a concentration of 20 mg / mL, absorb at a constant temperature for 15 h, centrifuge, and dry the precipitate for later use.

[0030] 3) Preparation of drug-loaded black phosphorus / sodium alginate composite microspheres: Add 100 g of cyclohexane and 12 g of dispersion stabilizer (a mixture of Span 60 and Tween 60, the mass ratio is 3:1) into the three-necked flask ) high-speed stirring (2000 rpm) until the dispersion stabilizer is completely dissolved, and solut...

Embodiment 3

[0033] 1) Preparation of black phosphorus nanosheets: block black phosphorus was ground into powder in a mortar and then added to N-methylpyrrolidone solvent (concentration: 1 mg / mL). After 9 hours of variable frequency ultrasound (45HZ, 80HZ, 100HZ) in an ice-water bath, centrifuge at a speed of 10,000 rpm to obtain black phosphorus nanosheet precipitates, and dry the precipitates for later use.

[0034] 2) Preparation of drug-loaded black phosphorus: Weigh 5 mg of black phosphorus nanosheets and add them to the ibuprofen solution with a concentration of 15 mg / mL, absorb at a constant temperature for 20 h, centrifuge, and dry the precipitate for later use.

[0035] 3) Preparation of drug-loaded black phosphorus / sodium alginate composite microspheres: add 100 g of cyclohexane to a three-necked flask, add 10 g of a dispersion stabilizer (a mixture of Span 60 and Tween 60, the mass ratio is 3:1 ), high-speed stirring (2000 rpm) until the dispersion stabilizer is completely disso...

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Abstract

The invention discloses a preparation method for a polylactic acid nanofiber scaffold functionally modified by two-dimensional nanometer black phosphorus, and aim to provide a preparation method for amultifunctional modified polylactic acid scaffold. The preparation method is characterized in that a black phosphorus nanosheet is taken as a medicine carrier to load an anti-inflammatory medicine, i.e., ibuprofen, a natural high polymer material, i.e., sodium alginate, is taken as a coating material, and through a crosslinking function of strontium ions, a medicine-carrying microsphere is obtained; an electrostatic interaction is used for evenly doping a sodium alginate medicine-carrying microsphere into a modified polylactic acid basis material which is subjected to ammonolysis and modification by branched polyethyleneimine and is rich in an active amino group; and through freezing phase separation, the modified polylactic acid scaffold with a reticular nanometer fiber structure is obtained. The preparation method has the characteristics that the prepared modified polylactic acid nanofiber scaffold embedded with the medicine-carrying microsphere is a multifunctional system with thefunctions of performing photothermal therapy, accelerating bone growth, resisting bacteria, diminishing inflammation and intelligently releasing medicines, and the polylactic acid nanofiber scaffold is an ideal bone repairing material.

Description

technical field [0001] The invention relates to a preparation method of a polylactic acid nanofiber scaffold modified by functional modification of two-dimensional nanometer black phosphorus, which belongs to the preparation technology of biological materials for bone repair. Background technique [0002] Bone tissue engineering is one of the effective measures to treat orthopedic diseases developed in recent years, in which cells, growth factors and scaffold materials are the three necessary components. Due to its multi-level pore structure and multiple components, the composite scaffold can imitate the structure and components of the extracellular matrix of natural bone tissue, and promote cell adhesion, proliferation and differentiation. Therefore, the materials used to prepare composite scaffolds should be strictly selected according to their biocompatibility, biodegradability and mechanical properties. Polylactic acid, a synthetic polymer material, is widely used in th...

Claims

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Application Information

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IPC IPC(8): A61L27/18A61L27/12A61L27/20A61L27/54A61K41/00A61P19/08C08G63/91
CPCA61K41/0052A61L27/12A61L27/18A61L27/20A61L27/54A61L2300/112A61L2300/21A61L2300/41A61L2300/412A61L2300/602A61L2300/622A61L2400/12A61L2430/02A61P19/08C08G63/912C08L5/04C08L67/04
Inventor 陈顺玉郭荣英肖秀峰
Owner 福建渤海传芳企业发展有限公司
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