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Preparation method and application of antibacterial repair type electrostatic spinning collagen-bacterial cellulose composite nanofiber scaffold

A technology of composite nanofibers and bacterial cellulose, applied in cellulose/protein conjugated rayon, fiber treatment, rayon manufacturing, etc., to achieve the effect of promoting adhesion

Active Publication Date: 2014-04-30
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, there are very few commodities that use electrospun bacterial cellulose as a matrix material, and can also be used as a tissue engineering scaffold to promote tissue repair and effectively inhibit surgical infection.

Method used

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  • Preparation method and application of antibacterial repair type electrostatic spinning collagen-bacterial cellulose composite nanofiber scaffold

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] (1) Preparation of mixed solvent: first prepare the ionic liquid 1-allyl-3-methylimidazole chloride (AMIMCL), add methylimidazole and allyl chloride with a volume ratio of 1:1.1 into a two-necked flask, Protect under nitrogen for five minutes, then heat and reflux at a constant temperature in an oil bath for a certain period of time; extract the reactant several times with anhydrous ether, then add activated carbon and deionized water, and conduct the reaction again at a certain temperature in the oil bath After reflux for several hours, a pale yellow liquid was obtained through diatomaceous earth, which was evaporated in vacuo, and then dried in vacuo for 48 hours to obtain AMIMCL; then 4-methylmorpholine-N-oxide (NMMO) and the obtained (AMIMCL) is mixed according to the mass ratio of 1:10, and the mass fraction of NMMO in the mixed system is 9% respectively;

[0031] (2) Preparation of bacterial cellulose solution: Dissolve 0.05g of bacterial cellulose in 10ml...

Embodiment 2

[0039] (1) Preparation of mixed solvent: first prepare ionic liquid 1-allyl-3-methylimidazole chloride (AMIMCL), add methylimidazole and allyl chloride with a volume ratio of 1:1.2 into a two-necked flask, Protect under nitrogen for five minutes, then heat and reflux at a constant temperature in an oil bath for a certain period of time; extract the reactant several times with anhydrous ether, then add activated carbon and deionized water, and conduct the reaction again at a certain temperature in the oil bath After reflux for several hours, a pale yellow liquid was obtained through diatomaceous earth, which was evaporated in vacuo, and then dried in vacuo for 48 hours to obtain AMIMCL; then 4-methylmorpholine-N-oxide (NMMO) and the obtained (AMIMCL) is mixed according to the mass ratio of 3:7, and the mass fraction of NMMO in the mixed system is 30% respectively;

[0040] (2) Preparation of bacterial cellulose solution: Dissolve 1g of bacterial cellulose in the above 10ml ...

Embodiment 3

[0048] (1) Preparation of mixed solvent: first prepare ionic liquid 1-allyl-3-methylimidazole chloride (AMIMCL), add methylimidazole and allyl chloride with a volume ratio of 1:1.6 into a two-necked flask, Protect under nitrogen for five minutes, then heat and reflux at a constant temperature in an oil bath for a certain period of time; extract the reactant several times with anhydrous ether, then add activated carbon and deionized water, and conduct the reaction again at a certain temperature in the oil bath After reflux for several hours, a pale yellow liquid was obtained through diatomaceous earth, which was evaporated in vacuo, and then dried in vacuo for 48 hours to obtain AMIMCL; then 4-methylmorpholine-N-oxide (NMMO) and the obtained (AMIMCL) is mixed according to the mass ratio of 7:3, and the mass fraction of NMMO in the mixed system is 70% respectively;

[0049] (2) Preparation of bacterial cellulose solution: Dissolve 1g of bacterial cellulose in 10ml of the abo...

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Abstract

The invention discloses a preparation method of an antibacterial repair type electrostatic spinning collagen-bacterial cellulose composite nanofiber scaffold. The method comprises the following steps: preparing a mixed solvent, dissolving bacterial cellulose and collagen, preparing a bacterial cellulose and collagen mixed solution containing AgNO3 or gold and silver nanocluster, spinning the bacterial cellulose and collagen mixed solution containing AgNO3 or gold and silver nanocluster and other fluorescent nanomaterials by adopting an electrostatic spinning technology, and reducing a composite fiber containing the AgNO3 or gold and silver nanocluster to obtain a nanofiber scaffold containing the nano-silver or gold and silver fluorescent nanomaterials by adopting ultraviolet light illumination, wherein the related composite nanofiber scaffold is used for cell culture to prepare a tissue engineering material. Different bacterial celluloses and collagens are proportioned, and the prepared composite nanofiber scaffold realizes adjustable mechanical strength and in-vivo degrading capability and can be used for preparing tissue engineering materials of multiple purposes.

Description

technical field [0001] The invention belongs to the field of preparation of bacterial cellulose composite nanofiber support, and in particular relates to a preparation method of an antibacterial repair type electrostatic spinning collagen-bacterial cellulose nanofiber support. Background technique [0002] Tissue engineering is a nascent technology that combines cell biology and materials science. Its main purpose and method are to prepare a three-dimensional extracellular matrix material with good biocompatibility and can be gradually degraded and absorbed by the human body. Cells are planted in the exogenous extracellular matrix and cultured in a bioreactor. Amplify to form a biomaterial-cell complex, and then implant this complex into the desired part of the body to replace the defective tissue or organ. Therefore, finding a suitable exogenous extracellular matrix to simulate the function of extracellular matrix molecules in natural tissues is of great significance for t...

Claims

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Application Information

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IPC IPC(8): A61L27/44A61L27/20A61L27/24A61L27/54A61L27/56A61L27/58D01F8/02D01F1/10D06M10/00
Inventor 王雪梅刘晓丽来兰梅
Owner SOUTHEAST UNIV
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